Biofilm-forming lactic acid bacteria of honey bee origin intended for potential probiotic use.

Scientists around the world are focusing their interest on the use of probiotics in honey bees as an alternative method of prophylaxis against causative agents of both American and European foulbrood. In our study we tested inhibitory activity against Paenibacillus larvae and the biofilm formation activity by various lactic acid bacteria isolated from honey bee guts or fresh pollen samples in the presence of different sugars added to the cultivation media. In addition, we tested the probiotic effect of a newly selected Apilactobacillus kunkeei V18 in an in situ experiment in bee colonies.

Effect of Application of Probiotic Pollen Suspension on Immune Response and Gut Microbiota of Honey Bees (Apis mellifera).

Although the use of probiotic bacteria in invertebrates is still rare, scientists have begun to look into their usage in honey bees. The probiotic preparation, based on the autochthonous strain Lactobacillus brevis B50 Biocenol™ (CCM 8618), which was isolated from the digestive tracts of healthy bees, was applied to the bee colonies in the form of a pollen suspension. Its influence on the immune response was determined by monitoring the expression of genes encoding immunologically important molecules in the honey bee intestines.

Changes of total protein and protein fractions in broiler chickens during the fattening period.

Background And Aim: Blood proteins in birds serve as an important indicator in the evaluation of health status and represent a basis in general biochemistry allowing the identification of metabolic alterations. The objective of this study was to evaluate the protein profile in broiler chickens extended by the concentrations of serum protein fractions at different periods of fattening.

Materials And Methods: Into the evaluation, we included 24 clinically healthy Ross 308 line meat-type chickens at the age of 2 days.