Publications by authors named "Bogdana Kravchuk"
Omsk hemorrhagic fever virus (OHFV) is a member of the tick-borne encephalitis virus (TBEV) complex of the family. Currently, there are no data on the cross-reactivity of antibodies to the NS1 proteins of OHFV and TBEV. Such data are of major interest for monitoring viral encephalitis of unknown etiology due to the increasing geographical distribution of OHFV.
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- The flaviviral NS1 glycoprotein is crucial in tick-borne encephalitis (TBE) pathology and could serve as a biomarker for early diagnosis and a target for therapy.
- Eukaryotic expression systems are typically used to produce NS1, but attempts to express it in Escherichia coli were largely unsuccessful due to solubility and folding issues.
- This study successfully produced soluble Trx-fused TBEV NS1 in E. coli by modifying cultivation conditions, and both soluble and refolded proteins exhibited immunological properties similar to native NS1, confirmed through various analysis methods.
Bacteriophages are promising antibacterial agents. Although they have been recognized as bacterial viruses and are considered to be non-interacting with eukaryotic cells, there is growing evidence that phages may have a significant impact on the immune system via interactions with macrophages, neutrophils, and T-cell polarization. In this study, the influence of phages of podovirus, siphovirus, and myovirus morphotypes on humoral immunity of CD-1 mice was investigated.
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