[Characteristics of the effect of implantation of the human ApoA1 gene on the condition of hepatocytes in rats of varying age].

The results obtained show the essential changes in functional state of hepatocyte's plasmatic membrane due to the implantation of human ApoA1 gene to the rat liver. The changes in phospholipid composition, hyperpolarization, increase in activity of membrane bound enzymes, cytochrome P-450 and biosynthesis of liver total proteins have been found. The essential changes characterizing cell effect were more marked in the adult rats, and membrane effect in the old ones.

[The vasoactive properties of different classes of blood lipoproteins in hypercholesterolemia and atherosclerosis].

Studies were made of the effects of various classes of lipoproteins (LP) from the blood of rabbits with experimental hypercholesterolemia and human subjects with pronounced atherosclerosis on the vascular tone of rat thoracic aorta specimens. In hypercholesterolemia, there was a considerable weakening of a vasodilatory action of atherogenic LP fractions--LDL and VLDL. A vasodilatory effect of HDLP was not changed.

[The effect of the implantation of the human apo A-I gene on the function of the hepatocyte plasma membrane in rats].

Evidences are given that implantation of human apo A-I gene to experimental animals induces essential changes in the functional state of hepatocyte plasmatic phospholipid composition, hyperpolarization development, as well as changes in the Na, K-ATPase and adenylate cyclase activities. The presence of generalized cell reaction is evidenced from the fact than biosynthesis of summary proteins gets enhanced during the above changes.

[High density lipoproteins in the blood of rats of varying age].

The study revealed age-related changes in the content and ratio of apoproteins which are part of high-density lipoproteins (HDL), as well as the development of dysapolipoproteinemia. The most significant fall (by 46.7%) was found in the content of the key HDL apoprotein--apoA1, and rise (by 107%)--in apoE.

[Use of enterosorption for correction of dyslipoproteinemias in patients with ischemic heart disease in geriatric practice].

Sixty patients with coronary heart disease, stable angina pectoris of functional class II and III and dislipoproteinemia (cholesterol greater than 6.5 mmol/l, triglycerides greater than 2.0 mmol/l, the atherogenic index greater than 3.

[Characteristics of lipid composition and properties of the synaptic membranes of the cerebral cortex of rats of various ages].

The content of cholesterol, total and individual phospholipids, fatty acid composition, level of lipid peroxidation, as well as viscosity of lipid phase of synaptic membranes isolated from the cerebral cortex were estimated in experiments on adult and old male rats. The content of cholesterol and cholesterol phospholipids ratio were found to increase with age. The total content of phospholipids remained unchanged during ageing, while their composition varied.

[Lipid composition and properties of plasma membranes in aging and certain types of experimental pathology].

To study the age-related relationship between the membrane status and activity of membrane-linked enzymes, the physicochemical properties of plasma membranes and activity of transfer ATPase and adenylate cyclase were assessed in tissues of rats of different ages. The lipid matrix of membranes was found to undergo appropriate changes with age. The most significant shifts are manifested under functional and metabolic stresses.

[Inhibitors of protein biosynthesis and development of dyslipoproteinemia in rabbits].

Acute alimentary hypercholesterolemia, developing after a single load with cholesterol, and experimental atherosclerosis, caused by long-term loading with cholesterol, were studied in young rabbits. Olivomycin, administered simultaneously with long-term cholesterol loading, and streptomycin, injected within 3 days before cholesterol loading, were used as inhibitors. These antibiotics prevented hypercholesterolemia, hyperlipoproteinemia and atherosclerosis development.

[Correction of dyslipoproteinemia in middle-aged and elderly patients with atherosclerosis using Essentiale in combination with adebit].

A combination of Essentiale and adebit was used in the treatment of 62 patients (age: 60-89 years) with stable stenocardia. Essentiale normalizes the cholesterol metabolism, adebit normalizes the carbohydrate metabolism. Results of the treatment indicate that elderly and old, in particular, showed an improvement of the clinical condition of the disease, normalization of the lipid metabolism.

[The effect of pH and pO2 changes in the blood on the energy metabolism of human erythrocytes in old age].

Changes in pH and pO2 of the blood have been studied for age peculiarities of their effect on the glycolysis rate and the content of ATP and 2,3-diphosphoglycerate (2,3-DPG) in erythrocytes (in vitro). The fresh venous blood of practically healthy young (aged 20-29) and old (aged 75-85) people was used. Acidosis was shown to induce inhibition of glycolysis and decrease of the ATP and 2.

[Mechanism of changes in the rate of glycolysis and levels of ATP and 2,3-diphosphoglycerate in human erythrocytes during aging].

Reasons which have induced changes in the glycolysis rate, ATP and 2,3-diphosphoglycerate content in human erythrocytes with ageing are studied. A fall of the hexokinase activity is shown to be one of the reasons of a significant decrease in the glycolysis rate. The total ATPase activity in erythrocytes does not change with the age.

[Age-related changes in glycolysis and levels of modulators of oxygen transport function of hemoglobin in human erythrocytes].

The glycolysis rate and the amount of the acid-transport hemoglobin function modulators (diphosphoglycerate (2,3-DPG), ATP, glutathione, chlorides) in human erythrocytes are studied under the effect of age changes. It is shown that the glycolysis rate and the content of ATP decrease under ageing by 16% and 21%, respectively. The concentration of 2,3-DPG lowers insignificantly (by 8%).

[Incorporation of radioactive phosphorus into creatine phosphate of the rabbit myocardium in experimental coronary insufficiency].

It is shown that under conditions of experimental limitation of coronary blood flow in rabbits the intensity of radioactive label (32P) incorporation into creatine phosphate lowers essentially. This evidences for inhibition of creatine phosphorylation in mitochondria under ischemia due to a decrease in the activity of a mitochondrial isoenzyme of creatine kinase.

[Age characteristics of lipoprotein lipase activity and cholesterol levels in various lipoprotein fractions].

In practically healthy from 20 up to 95 years old persons, independently of sex, only slight alterations in the mean content of total cholesterol were noted. However, redistribution of cholesterol occurred between various classes of lipoproteins. There was a decrease of cholesterol content in high density lipoproteins (LPHD) and an increase in cholesterol content in lipoproteins of very low and low density.

[Effect of vasopressin on energy metabolism of the brain in adult and aged rats].

Content of adenosine phosphate, creatine phosphate, Pi, lactate and ATPase activity were studied in brain of 8-10- and 25-27-months old rats. Content of ATP and ADP and the Na+, K+-ATPase activity were decreased while the level of Pi was increased in rat brain cortex during ageing. Administration of vasopressin caused an increase in lactate, Pi and Na+, K+-ATPase activity, whereas creatine phosphate decreased in the adult rat hypothalamus.