Assay Development and Validation for Innovative Antiviral Development Targeting the N-Terminal Autoprocessing of SARS-CoV-2 Main Protease Precursors.

The SARS-CoV-2 main protease (M) is initially synthesized as part of polyprotein precursors that undergo autoproteolysis to release the free mature M. To investigate the autoprocessing mechanism in transfected mammalian cells, we examined several fusion precursors, with the mature SARS-CoV-2 M along with the flanking amino acids (to keep the native substrate sequences) sandwiched between different tags. Our analyses revealed differential proteolysis kinetics at the N- and C-terminal cleavage sites.

Hormonal contraception.

Increased sexual activity among adolescents demands that the health care provider have a thorough understanding of contraceptive methods and application. In this article, the principles of hormonal contraception are discussed, providing relevant information for use in advising and managing the sexually active adolescent.

Toxic shock-like syndrome associated with Bartholin's gland abscess: case report.

Herein is the first reported case of bartholinitis complicated by toxic shock-like syndrome. Streptococcal exotoxin is implicated in this case of nonmenstrual toxic shock-like syndrome, which is an increasingly recognized manifestation of streptococcal infection. Further surveillance, investigation, and reporting of streptococcal toxic shock-like syndrome are recommended.