Cytokine profile in children with inflammatory bowel disease.

Crohn's disease (CD) and ulcerative colitis (UC) are inflammatory bowel diseases (IBD) characterized by immune reactivity against microbial and auto-antigens. This work was designed to study the cytokine profile in blood serum and coproextracts of children with CD and UC. The studied patients consisted of 17 children with CD (group I), 17 children with UC (group II), and 18 controls with intestinal dysbiosis (group III).

[Evaluation of the immunological activity and safety of group B meningococcal vaccine prepared from a natural complex of specific polysaccharide and outer membrane proteins].

Immunological activity and safety of group B meningococcal vaccine prepared from a natural complex of specific polysaccharide and outer membrane proteins were under study. The immunological safety of the vaccine was evaluated by the absence of antibodies to denaturated and native DNA (d-DNA and n-DNA). As shown with the use of the enzyme immunoassay (EIA), the administration of the vaccine did not induce antibody formation to d-DNA and n-DNA during the observation period.

[The molecular parameters of a meningococcal serogroup B protein-polysaccharide vaccine].

Methods for the evaluation of the molecular parameters of B polysaccharide (B-PS) in meningococcal protein-polysaccharide vaccine of group B are proposed. The comparison of two proposed methods, the passive hemagglutination inhibition test and rocket immunoelectrophoresis (RIEP), has shown that the latter method has the highest degree of correlation with the chemical method of the detection of B-PS, which is often hindered by lactose added as a bulking agent. RIEP may be recommended for the standardization and control of the commercial preparations of group B meningococcal vaccine.

[The effect of the conditions for Neisseria meningitidis cultivation on the protein composition in the protein-polysaccharide complex--the base of meningococcal serogroup B vaccine].

New data concerning the influence of cultivation conditions on the synthesis aimed at producing immunologically active antigens of group B Neisseria meningitides were obtained. The combination of conditions (deficiency in ions of iron, pH of the medium, growth phase of the culture) capable of essentially increasing (9- to 28-fold) the content of iron-dependent protein with a mol. wt.

[The relationship between the immunological efficacy of a dried meningococcal group-A polysaccharide vaccine and the molecular parameters of the group-A polysaccharide].

This work deals with the problem of relationship between the molecular parameters of group A meningococcal polysaccharide and its immunological effectiveness for laboratory animals and humans. The depolymerization of group A polysaccharide contained in the vaccine leads to a decrease in its capacity of inducing the production of hemagglutinating (19S and 7S) and bactericidal IgA antibodies in humans, as well as inducing an increase in the number of cells producing IgA antibodies in the spleen of immunized mice and the appearance of circulating IgA antibodies in their sera. As shown in this investigation, fully developed immune response to group A meningococcal vaccine may be achieved in humans only if the content of group A high-molecular polysaccharide in the vaccine is not less than 70%.

[The protective activity of polyclonal and monoclonal antibodies to the lipopolysaccharide of Neisseria meningitidis serogroup A in in vivo experiments].

The protective activity of the sera of mice immunized with the preparations of native and detoxified N. meningitidis lipopolysaccharide (LPS), group A, as well as with monoclonal antibodies to N. meningitidis antigens, groups A and B, was studied on the mucin model of meningococcal infection.

[The protective activity of the detoxified lipopolysaccharide of Neisseria meningitidis serogroup A in in vivo experiments].

The immunogenic potency, toxicity, homologous and heterologous protective activity of lipopolysaccharide preparations obtained from serogroup A N. meningitidis (LPS A) were studied in animal experiments. These preparations were shown to possess very high protective activity.

[The course of protracted reactive depression in young children].

Clinical-dynamic and catamnestic techniques were used to study 34 children with protracted reactive depression developed as a response to their maladaptation to the nursing house conditions. The following stages of the disease were singled out: I, acute affective-shock responses; II, subacute reactive depression; III, protracted polymorphic depressive-neurotic state; IV, regress of the disease, compensation; V, post-reactive++ state, the outcome of the psychogenic disorders. The psychopathological and psychological characterization of each stage was performed.

[Evaluation of the effectiveness of group-A and -C meningococcal polysaccharide vaccines by molecular parameters].

Chromatographic properties of meningococcal polysaccharides and vaccines of serogroups A and C prepared at the G. N. Gabrichevskiĭ Moscow Research Institute of Epidemiology and Microbiology were studied during determination of their molecular parameters by gel filtration on sepharose 4B.

[Psychopathologic structure of reactive depression in young children].

Thirty-four children, aged 1 to 3.5 years, with a reactive depressive state were examined. Using the ontogenetic approach, the authors analyze affective as well as somatovegetative, psychomotor and behavioural disorders in the structure of the abnormal state.

[Variants of movement disorders within the psychopathologic structure of borderline neuropsychiatric diseases in young children].

On the basis of the examination covering 30 young children (aged 2 months to 3.5 years), three variants of motor psychogenic disorders were specified. Group I included elementary, stereotypic motor phenomena expressed as special poses, rocking, jack-knifing, finger sucking and other stereotypes of motor acts.