A label-free quantification method for assessing sex from modern and ancient bovine tooth enamel.

Identification of the sex of modern, fossil and archaeological animal remains offers many insights into their demography, mortality profiles and domestication pathways. However, due to many-factors, sex determination of osteological remains is often problematic. To overcome this, we have developed an innovative protocol to determine an animal's sex from tooth enamel, by applying label-free quantification (LFQ) of two unique AmelY peptides 'LRYPYP' (AmelY;[M+2] 404.

Radiocarbon chronology of Iron Age Jerusalem reveals calibration offsets and architectural developments.

Reconstructing the absolute chronology of Jerusalem during the time it served as the Judahite Kingdom's capital is challenging due to its dense, still inhabited urban nature and the plateau shape of the radiocarbon calibration curve during part of this period. We present 103 radiocarbon dates from reliable archaeological contexts in five excavation areas of Iron Age Jerusalem, which tie between archaeology and biblical history. We exploit Jerusalem's rich past, including textual evidence and vast archaeological remains, to overcome difficult problems in radiocarbon dating, including establishing a detailed chronology within the long-calibrated ranges of the Hallstatt Plateau and recognizing short-lived regional offsets in atmospheric C concentrations.

Sub-annual bomb radiocarbon records from trees in northern Israel.

Spatial and temporal variations in the atmospheric bomb radiocarbon make it a very useful tracer and a dating tool. With the introduction of more atmospheric bomb radiocarbon records, the spatial and temporal changes in bomb radiocarbon are becoming clearer. Bomb radiocarbon record from a pine tree in the northern Israel region shows that the ΔC level in the region is closer to the northern hemisphere zone (NH) 1 as compared to the northern hemisphere zone (NH) 2.

Crystallinity assessment of anthropogenic calcites using Raman micro-spectroscopy.

Anthropogenic calcite is a form of calcium carbonate produced through pyrotechnological activities, and it is the main component of materials such as lime binders and wood ash. This type of calcite is characterized by a significantly lower degree of crystallinity compared with its geogenic counterparts, as a result of different formation processes. The crystallinity of calcite can be determined using infrared spectroscopy in transmission mode, which allows decoupling particle size effect from atomic order and thus effectively distinguish anthropogenic and geogenic calcites.

Three dimensional structures of the inner and outer pig petrous bone using FIB-SEM: Implications for development and ancient DNA preservation.

We report on the 3D ultrastructure of the mineralized petrous bone of mature pig using focused ion beam - scanning electron microscopy (FIB-SEM). We divide the petrous bone into two zones based on the degree of mineralization; one zone close to the otic chamber has higher mineral density than the second zone further away from the otic chamber. The hypermineralization of the petrous bone results in the collagen D-banding being poorly revealed in the lower mineral density zone (LMD), and absent in the high mineral density zone (HMD).

Ancient DNA from a lost Negev Highlands desert grape reveals a Late Antiquity wine lineage.

Recent excavations of Late Antiquity settlements in the Negev Highlands of southern Israel uncovered a society that established commercial-scale viticulture in an arid environment [D. Fuks , , 19780-19791 (2020)]. We applied target-enriched genome-wide sequencing and radiocarbon dating to examine grapevine pips that were excavated at three of these sites.

Early Upper Paleolithic cultural variability in the Southern Levant: New evidence from Nahal Rahaf 2 Rockshelter, Judean Desert, Israel.

The Levantine Early Upper Paleolithic (ca. 45-30 ka) has been a focus of research because of its unique position as a conduit of human, flora, and fauna species between Africa and Eurasia. Studies have mainly focused on the Early Ahmarian and Levantine Aurignacian, two entities, the former endemic and the latter foreign, which are considered to have coinhabited the region during that period.

The petrous bone contains high concentrations of osteocytes: One possible reason why ancient DNA is better preserved in this bone.

The characterization of ancient DNA in fossil bones is providing invaluable information on the genetics of past human and other animal populations. These studies have been aided enormously by the discovery that ancient DNA is relatively well preserved in the petrous bone compared to most other bones. The reasons for this better preservation are however not well understood.

Expansion of eastern Mediterranean Middle Paleolithic into the desert region in early marine isotopic stage 5.

Marine Isotopic Stage 5 is associated with wetter climatic conditions in the Saharo-Arabian deserts. This stage also corresponds to the establishment of Middle Paleolithic hominins and their associated material culture in two geographical provinces in southwest Asia-the Eastern Mediterranean woodland and the Arabian Peninsula desert. The lithic industry of the Eastern Mediterranean is characterized by the centripetal Levallois method, whereas the Nubian Levallois method characterizes the populations of the Arabian desert.

The absolute chronology of Boker Tachtit (Israel) and implications for the Middle to Upper Paleolithic transition in the Levant.

The Initial Upper Paleolithic (IUP) is a crucial lithic assemblage type in the archaeology of southwest Asia because it marks a dramatic shift in hominin populations accompanied by technological changes in material culture. This phase is conventionally divided into two chronocultural phases based on the Boker Tachtit site, central Negev, Israel. While lithic technologies at Boker Tachtit are well defined, showing continuity from one phase to another, the absolute chronology is poorly resolved because the radiocarbon method used had a large uncertainty.

Early production of table olives at a mid-7th millennium BP submerged site off the Carmel coast (Israel).

We present here the earliest evidence for large-scale table olive production from the mid-7 millennium BP inundated site of Hishuley Carmel on the northern Mediterranean coast of Israel. Olive pit size and fragmentation patterns, pollen as well as the architecture of installations associated with pits from this site, were compared to finds from the nearby and slightly earlier submerged Kfar Samir site. Results indicate that at Kfar Samir olive oil was extracted, while at Hishuley Carmel the data showed that large quantities of table olives, the oldest reported to date, were prepared.

Discovery of annual growth in a modern olive branch based on carbon isotopes and implications for the Bronze Age volcanic eruption of Santorini.

The volcanic eruption of Santorini in the Bronze Age left detectable debris across the Mediterranean, serving as an anchor in time for the region, synchronizing chronologies of different sites. However, dating the eruption has been elusive for decades, as radiocarbon indicates a date about a century earlier than archaeological chronologies. The identification of annual rings by CT in a charred olive branch, buried alive beneath the tephra on Santorini, was key in radiocarbon dating the eruption.

Site formation processes at Manot Cave, Israel: Interplay between strata accumulation in the occupation area and the talus.

Manot Cave contains important human fossils and archaeological assemblages related to the origin and dispersal of anatomically modern humans and the Upper Paleolithic period. This record is divided between an elevated in situ occupation area and a connecting talus. We, thus, investigated the interplay between the accumulation of the sediments and their associated artifacts in the occupation areas and the translocation of part of these sediments and artifacts down the talus.

The Marine Isotope Stage 3 landscape around Manot Cave (Israel) and the food habits of anatomically modern humans: New insights from the anthracological record and stable carbon isotope analysis of wild almond (Amygdalus sp.).

The excavation of Manot Cave (Israel) reveals intensive occupation during the Early Upper Paleolithic and provides the first continuous set of anthracological data available for the Ahmarian, Levantine Aurignacian and post-Levantine Aurignacian periods. The paper aims to study the vegetal landscape around Manot Cave in the context of climate changes that characterized the last part of the Marine Isotope Stage 3 (MIS 3) and to address the issue of firewood and food procurement among Paleolithic hunter-gatherers. Charcoal samples recovered from the archaeological layers at Manot Cave shed light on the fuel and food procurement strategies while radiocarbon dating and stable carbon isotope analysis (ΔC) of selected charcoals provide information about the ancient climate.

Radiocarbon dating and microarchaeology untangle the history of Jerusalem's Temple Mount: A view from Wilson's Arch.

Radiocarbon dating is rarely applied in Classical and Post-Classical periods in the Eastern Mediterranean, as it is not considered precise enough to solve specific chronological questions, often causing the attribution of historic monuments to be based on circumstantial evidence. This research, applied in Jerusalem, presents a novel approach to solve this problem. Integrating fieldwork, stratigraphy, and microarchaeology analyses with intense radiocarbon dating of charred remains in building materials beneath Wilson's Arch, we absolutely dated monumental structures to very narrow windows of time-even to specific rulers.

Precipitation variability differently affects radial growth, xylem traits and ring porosity of three Mediterranean oak species at xeric and mesic sites.

In the Mediterranean basin, diffuse-porous, semi-ring-porous and ring-porous tree species coexist in the same regions. Climate change might differently affect these types, but a mechanistic understanding of drought effects on their xylem structure is lacking. We investigated tree-ring width and xylem functional traits in ring-porous Quercus boissieri, semi-ring-porous Q.

Climatic and environmental conditions in the Western Galilee, during Late Middle and Upper Paleolithic periods, based on speleothems from Manot Cave, Israel.

Early Ahmarian, Levantine Aurignacian and Post-Levantine Aurignacian archeological assemblages show that the karstic Manot Cave, located 5 km east of the Mediterranean coast in the Western Galilee region of Israel, was intensively occupied during the Early Upper Paleolithic. The coexistence of these rich archaeological layers with speleothems in Manot Cave provides a window of opportunity for determining the relationships between climatic conditions and the nature of human activity and mobility patterns in the Western Galilee region during the Early Upper Paleolithic period. This study, based on four stalagmites that grew almost continuously from ∼75 to 26.

Ancient trash mounds unravel urban collapse a century before the end of Byzantine hegemony in the southern Levant.

The historic event of the Late Antique Little Ice Age (LALIA) was recently identified in dozens of natural and geological climate proxies of the northern hemisphere. Although this climatic downturn was proposed as a major cause for pandemic and extensive societal upheavals in the sixth-seventh centuries CE, archaeological evidence for the magnitude of societal response to this event is sparse. This study uses ancient trash mounds as a type of proxy for identifying societal crisis in the urban domain, and employs multidisciplinary investigations to establish the terminal date of organized trash collection and high-level municipal functioning on a city-wide scale.

Radiocarbon analysis of modern olive wood raises doubts concerning a crucial piece of evidence in dating the Santorini eruption.

Charred olive wood is abundant in the archaeological record, especially around the Mediterranean. As the outermost ring closest to the bark is assumed to represent the latest time that the tree was alive, the radiocarbon date obtained from the outermost rings of an olive branch buried during the Santorini volcanic eruption is regarded as crucial evidence for the date of this cataclysmic event. The date of this eruption has far reaching consequences in the archaeology of the Aegean, Egypt and the Levant, and the understanding of their interconnections.

High Resolution AMS Dates from Shubayqa 1, northeast Jordan Reveal Complex Origins of Late Epipalaeolithic Natufian in the Levant.

The Late Epipalaeolithic Natufian (~14,600 - 11,500 cal BP) is a key period in the prehistory of southwest Asia. Often described as a complex hunting and gathering society with increased sedentism, intensive plant exploitation and associated with an increase in artistic and symbolic material culture, it is positioned between the earlier Upper- and Epi-Palaeolithic and the early Neolithic, when plant cultivation and subsequently animal domestication began. The Natufian has thus often been seen as a necessary pre-adaptation for the emergence of Neolithic economies in southwest Asia.

Radiocarbon Dating of an Olive Tree Cross-Section: New Insights on Growth Patterns and Implications for Age Estimation of Olive Trees.

The age of living massive olive trees is often assumed to be between hundreds and even thousands of years. These estimations are usually based on the girth of the trunk and an extrapolation based on a theoretical annual growth rate. It is difficult to objectively verify these claims, as a monumental tree may not be cut down for analysis of its cross-section.

Radiocarbon chronology of Manot Cave, Israel and Upper Paleolithic dispersals.

The timing of archeological industries in the Levant is central for understanding the spread of modern humans with Upper Paleolithic traditions. We report a high-resolution radiocarbon chronology for Early Upper Paleolithic industries (Early Ahmarian and Levantine Aurignacian) from the newly excavated site of Manot Cave, Israel. The dates confirm that the Early Ahmarian industry was present by 46,000 calibrated years before the present (cal BP), and the Levantine Aurignacian occurred at least between 38,000 and 34,000 cal BP.

Farming legumes in the pre-pottery Neolithic: New discoveries from the site of Ahihud (Israel).

New discoveries of legumes in the lower Galilee at the prehistoric site of Ahihud in Israel shed light on early farming systems in the southern Levant. Radiocarbon dating of twelve legumes from pits and floors indicate that the farming of legumes was practiced in southern Levant as early as 10.240-10.