is a common intracellular parasitic bacterium that infects humans via the respiratory tract, causing Legionnaires' disease, with fever and pneumonia as the main symptoms. The emergence of highly virulent and azithromycin-resistant is a major challenge in clinical anti-infective therapy. The CRISPR-Cas acquired immune system provides immune defense against foreign nucleic acids and regulates strain biological functions.
View Article and Find Full Text PDFBackground: Q fever endocarditis, a chronic illness caused by Coxiella burnetii, can be fatal if misdiagnosed or left untreated. Despite a relatively high positive rate of Q fever serology in healthy individuals in the mainland of China, very few cases of Q fever endocarditis have been reported. This study summarized cases of Q fever endocarditis among blood culture negative endocarditis (BCNE) patients and discussed factors attributing to the low diagnostic rate.
View Article and Find Full Text PDFRickettsia heilongjiangensis is an obligate intracellular bacterium that causes Far-Eastern tick-borne spotted fever. Outer membrane protein B (OmpB) is an important surface protein antigen of rickettsiae. In the present study, the ompB gene of R.
View Article and Find Full Text PDFIn total, 676 Dermacentor silvarum Olenev (Acari: Ixodidae) from a forest area of Jilin Province in northeastern China were examined by polymerase chain reaction for the presence of spotted fever group (SFG) Rickettsia. The overall positive rate was 10.7%, with a 95% confidence interval from 8.
View Article and Find Full Text PDFZhonghua Liu Xing Bing Xue Za Zhi
March 2007
Objective: To develop a quantitative real-time polymerase chain reaction (PCR) for detecting Bartonella henselae.
Methods: According to the 16S-23S rRNA intervening sequences (IVS) specific for B. henselae, one pair of primers and one TaqMan-MGB probe were designed.
Zhonghua Liu Xing Bing Xue Za Zhi
November 2006
Objective: To develop a quantitative real-time polymerase chain reaction (PCR) for detecting Rickettsia prowazekii.
Methods: Primers and TaqMan-MGB probes designed based on ompB gene of R. prowazekii, were used to develop this method.
Zhonghua Liu Xing Bing Xue Za Zhi
June 2006
Objective: To develop a real-time quantitative polymerase chain reaction(PCR) assay for detecting Rickettsia rickettsii.
Methods: The primers and TaqMan-MGB probe were designed according to the ompB gene of R. rickettsii.
The gene encoding the 56-kDa protein of Orientia tsutsugamushi Shanxi was amplified by a nested PCR and cloned into the expression vector pQE30. The 56-kDa protein of O. tsutsugamushi Shanxi (Sxh56) was expressed as a fusion protein with the His(6)-binding protein of Escherichia coli by deleting the signal peptide-encoding sequence from the 5' end of the open reading frame.
View Article and Find Full Text PDFA total of 1,345 Ixodes persulcatus ticks collected from northeastern China were investigated for the presence of Anaplasma phagocytophila and Borrelia burgdorferi by a nested polymerase chain reaction (PCR). Sixty-two (4.6%) ticks were positive for A.
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