Distribution characteristics of the CRISPR-Cas system and its regulatory mechanism underpinning phenotypic function.

is a common intracellular parasitic bacterium that infects humans via the respiratory tract, causing Legionnaires' disease, with fever and pneumonia as the main symptoms. The emergence of highly virulent and azithromycin-resistant is a major challenge in clinical anti-infective therapy. The CRISPR-Cas acquired immune system provides immune defense against foreign nucleic acids and regulates strain biological functions.

Retrospective Examination of Q Fever Endocarditis: An Underdiagnosed Disease in the Mainland of China.

Background: Q fever endocarditis, a chronic illness caused by Coxiella burnetii, can be fatal if misdiagnosed or left untreated. Despite a relatively high positive rate of Q fever serology in healthy individuals in the mainland of China, very few cases of Q fever endocarditis have been reported. This study summarized cases of Q fever endocarditis among blood culture negative endocarditis (BCNE) patients and discussed factors attributing to the low diagnostic rate.

Protective immunity against Rickettsia heilongjiangensis in a C3H/HeN mouse model mediated by outer membrane protein B-pulsed dendritic cells.

Rickettsia heilongjiangensis is an obligate intracellular bacterium that causes Far-Eastern tick-borne spotted fever. Outer membrane protein B (OmpB) is an important surface protein antigen of rickettsiae. In the present study, the ompB gene of R.

Molecular detection of spotted fever group Rickettsia in Dermacentor silvarum from a forest area of northeastern China.

In total, 676 Dermacentor silvarum Olenev (Acari: Ixodidae) from a forest area of Jilin Province in northeastern China were examined by polymerase chain reaction for the presence of spotted fever group (SFG) Rickettsia. The overall positive rate was 10.7%, with a 95% confidence interval from 8.

[Development of a quantitative real-time polymerase chain reaction for detecting Bartonella henselae].

Objective: To develop a quantitative real-time polymerase chain reaction (PCR) for detecting Bartonella henselae.

Methods: According to the 16S-23S rRNA intervening sequences (IVS) specific for B. henselae, one pair of primers and one TaqMan-MGB probe were designed.

[Detection of Rickettsia prowazekii by quantitative real-time PCR].

Objective: To develop a quantitative real-time polymerase chain reaction (PCR) for detecting Rickettsia prowazekii.

Methods: Primers and TaqMan-MGB probes designed based on ompB gene of R. prowazekii, were used to develop this method.

[Study on the development of a real-time quantitative polymerase chain reaction assay to detect Rickettsia].

Objective: To develop a real-time quantitative polymerase chain reaction(PCR) assay for detecting Rickettsia rickettsii.

Methods: The primers and TaqMan-MGB probe were designed according to the ompB gene of R. rickettsii.

Recombinant 56-kilodalton major outer membrane protein antigen of Orientia tsutsugamushi Shanxi and its antigenicity.

The gene encoding the 56-kDa protein of Orientia tsutsugamushi Shanxi was amplified by a nested PCR and cloned into the expression vector pQE30. The 56-kDa protein of O. tsutsugamushi Shanxi (Sxh56) was expressed as a fusion protein with the His(6)-binding protein of Escherichia coli by deleting the signal peptide-encoding sequence from the 5' end of the open reading frame.

Prevalence of Anaplasma phagocytophila and Borrelia burgdorferi in Ixodes persulcatus ticks from northeastern China.

A total of 1,345 Ixodes persulcatus ticks collected from northeastern China were investigated for the presence of Anaplasma phagocytophila and Borrelia burgdorferi by a nested polymerase chain reaction (PCR). Sixty-two (4.6%) ticks were positive for A.