Publications by authors named "Bo-Yin Qin"
Article Synopsis
- Humanized mouse models that replicate the human HLA system are critical for studying vaccine development and immune responses to infections, due to significant differences between animal and human immune systems.!* -
- Researchers created a transgenic mouse strain with a modified HLA-A30 monochain that incorporates parts of the human and mouse MHC, allowing them to assess immune responses to the influenza A virus (H1N1).!* -
- The study found that the new transgenic mice displayed a robust immune response similar to regular mice when infected with the H1N1 virus, indicating the effectiveness of the humanized model in immunological research.!*
Article Synopsis
- Research shows that HLA-DP, a lesser-known HLA class II antigen, plays important roles in cancer, allergies, and infections, but its function is poorly understood due to the lack of suitable animal models.
- The study involved creating transgenic mice carrying the complete HLA-DP401 gene locus to analyze gene expression and immune responses through various testing methods.
- Results indicated that while overall immune cell percentages remained unchanged after infection, there was an increased expression of HLA-DP401 in the thymus, suggesting a specific immune response mechanism linked to this antigen.
Reporter genes are widely applied in biotechnology and biomedical research owning to their easy observation and lack of toxicity. Taking advantage of the reporter genes in conjunction with imaging technologies, a large number of reporter mouse models have been generated. Reporter mouse models provide systems that enable the studies of live cell imaging, cell lineage tracing, immunological research and cancers etc.
View Article and Find Full Text PDFHuman liver chimeric mouse model based on diphtheria toxin-induced liver injury.
World J Gastroenterol
July 2017
Aim: To establish an inducible liver injury mouse model and transplant human hepatocytes to obtain liver-humanized mice.
Methods: We crossed three mouse strains, including albumin (Alb)-cre transgenic mice, inducible diphtheria toxin receptor (DTR) transgenic mice and severe combined immune deficient (SCID)-beige mice, to create Alb-cre/DTR/SCID-beige (ADSB) mice, which coincidentally harbor Alb-cre and DTR transgenes and are immunodeficient. As the Cre expression is driven by the liver-specific promoter Alb (encoding ALB), the DTR stop signal flanked by two loxP sites can be deleted in the ADSB mice, resulting in DTR expression in the liver.
Three series of resveratrol oligomer derivatives were synthesized, including the indenone-type, indene-type and octahydropentalene-type derivatives, among which ten derivatives were novel compounds. Compounds 2, 14f, and 4d were confirmed as ERβ agonists by yeast two-hybrid assay, and compound 2 (isopaucifloral F) was further chosen to evaluate its anti-osteoporosis activity in vivo. Compared with the sham-operated and the positive control groups, isopaucifloral F (10 μg/kg) showed a notable anti-osteoporosis effect in the ovariectomized (OVX) female rats based on a micro-CT analysis and the following measurements: bone mineral density, bone volume/tissue volume, trabecular thickness, trabecular separation/spacing, and the serum biochemical parameters.
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