Cloning and Analysis of Intron 3 of Human Telomerase Reverse Transcriptase (hTERT) Gene.

Total RNA was extracted from telomerase-positive cell line SPC-A, and reverse transcribed to cDNA. The long template PCR was performed using this cDNA as template and the hTERT cDNA specific oligonucleotides as primers. A long fragment of about 2.