Activation of N-acyl-homoserine lactone-mediated quorum sensing system improves long-term preservation of anammox microorganisms by vacuum lyophilization.

The long-term preservation of anaerobic ammonium oxidation (anammox) microorganisms via vacuum lyophilization process would help commercialize the technique. In this study, vacuum lyophilization was evaluated for the cost-effective long-term preservation of such microorganisms. Skim milk was found to be the most effective cryoprotectant for maintaining the physiological properties (heme c, EPS, and the PN/PS ratio) of anammox microorganisms.