Publications by authors named "Bo-Moon Shin"
Article Synopsis
- * Out of 3,397 bacterial isolates from 3,094 patients, 949 were classified as multidrug-resistant organisms (MDRO), highlighting significant resistance to commonly used antibiotics, including imipenem, methicillin, and vancomycin.
- * Although the antibiotic spectrum coverage (ASC) scores were similar for MDRO and non-MDRO groups initially, the MDRO group showed significant improvements in coverage two days later, indicating a need for faster diagnostic methods to combat emerging resistance.
Comparative evaluation of two molecular multiplex syndromic panels with acute gastroenteritis.
Diagn Microbiol Infect Dis
May 2024
We compared the Allplex Gastrointestinal V/B1/B2 Assays and Seeplex Diarrhea V/B1/B2 ACE Detection Assays in patients with acute gastroenteritis (AGE). Of the total 432 specimens, 48.8% and 54.
View Article and Find Full Text PDFBackground: The interest in infection (CDI) has increased, and the choice of assays became wider since the first national survey in Korea on CDI diagnosis in 2015. We conducted a survey of the domestic CDI assays with more varied questions to understand the current situation in Korea.
Methods: In April 2018, about 50 questions on the current status of CDI assays and details on implementation and perceptions were written, and a survey questionnaire was administered to laboratory medicine specialists in 200 institutions.
Background: Various methods are used for the diagnosis of infection (CDI). We systematically analyzed and investigated the performance of current laboratory diagnostic methods for CDI.
Methods: We performed systematic review and meta-analysis of studies in PubMed, Web of Science, Cochrane Library, and KoreaMed.
In May 2015, we conducted a voluntary online survey on laboratory diagnostic assays for infection (CDI) across clinical microbiology laboratories in Korea. Responses were obtained from 66 laboratories, including 61 hospitals and five commercial laboratories. Among them, nine laboratories reported having not conducted CDI assays.
View Article and Find Full Text PDFBackground: During the 2015 outbreak of Middle East Respiratory Syndrome coronavirus (MERS-CoV), six different commercial MERS-CoV RNA detection kits based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) were available in Korea. We performed analytical and clinical validations of these kits.
Methods: PowerChek (Kogene Biotech, Korea), DiaPlexQ (SolGent, Korea), Anyplex (Seegene, Korea), AccuPower (Bioneer, Korea), LightMix (Roche Molecular Diagnostics, Switzerland), and UltraFast kits (Nanobiosys, Korea) were evaluated.
We evaluated the performance of the VIDAS GDH assay for the detection of Clostridium difficile. In total, 350 fecal specimens collected from patients clinically suspected of having CDI were analyzed by C. difficile culture and enzyme-linked fluorescent immunoassay (VIDAS GDH); the results were compared with those of toxigenic C.
View Article and Find Full Text PDFBackground: We evaluated the performance of four commercial nucleic acid amplification tests (NAATs: Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C.
View Article and Find Full Text PDFArticle Synopsis
- * Out of 530 stool specimens, IDCd demonstrated significantly higher positivity rates on days 1 and 2 than CDSA, with a 90.6% concordance rate overall.
- * Although both media showed similar detection rates on day 3, IDCd may be more effective for rapid and sensitive detection of C. difficile within the first two days of culture.
Background: Pathogens in the nasal cavity during nasal surgery could lead to a systemic infectious condition, such as bacteremia, nosocomial infection, or toxic shock syndrome. However, there is no research about the prevalence of nasal carriage in patients with nasal bone fracture.
Methods: This was a prospective, double-blind, randomized study about the rate of nasal carriage in 200 patients with nasal bone fracture in Korea.
Article Synopsis
- - The study compared two PCR tests (BD GeneOhm and Seegene ACE) for detecting the tcdB gene from stool samples, finding a high agreement rate of 96.3% between the two methods.
- - BD GeneOhm demonstrated sensitivities of 95.7% and specificities of 96.5%, while Seegene ACE had sensitivities of 90.0% and specificities of 97.1%.
- - Positive predictive values (PPVs) were 91.8% for BD and 92.6% for Seegene, while negative predictive values (NPVs) were 98.2% and 96.0%, respectively.
Article Synopsis
- * The study compared the effectiveness of the DiversiLab™ system (rep-PCR) with traditional IS6110-RFLP analysis to identify Mycobacterium tuberculosis in 89 clinical isolates from Korea.
- * The DiversiLab system successfully grouped the isolates into 8 categories, showing quick and reliable results, and is suggested for molecular epidemiological studies of tuberculosis in Korea.
Article Synopsis
- C. difficile was identified in 36 out of 272 patients tested (13.2%), with isolation rates increasing annually from 2008 to 2010, and a significant percentage of toxin-positive cases being linked to either CO-HA-CDI or community-acquired CDI (CA-CDI).
- Key findings indicated that most CO-HA-CDI cases occurred within 2 weeks post-discharge, and the characteristics of CA-CDI cases included younger patients with fewer prior health issues and a diverse genetic background
Article Synopsis
- The nationwide external quality assessment (EQA) of the fecal occult blood test (FOBT) in Korea began between 2007 and 2009 to evaluate the test’s performance and improve quality control.
- Over the three years, a total of 650 hospitals participated, providing data on their test results and feedback on testing procedures; the majority of institutions used qualitative kits over quantitative methods.
- The results indicated varying consistency in test outcomes—with greater reliability noted in quantitative tests by 2009—but highlighted the need for standardization in testing procedures and further education for lab personnel to minimize discrepancies.
Background/aims: Clostridium difficile is the predominant cause of nosocomial diarrhea. Recently, the incidence of Clostridium difficile infection (CDI) increases in Europe and North America. A retrospective study was performed to evaluate the change of incidence and clinical features of CDI in Korea.
View Article and Find Full Text PDFPericentric inversion of chromosome 4 can give rise to 2 alternate recombinant (rec) chromosomesby duplication or deletion of 4p. The deletion of distal 4p manifests as Wolf-Hirschhorn syndrome (WHS). Here, we report the molecular cytogenetic findings and clinical manifestations observed in an infant with 46,XX,rec(4)dup(4q)inv(4)(p16q31.
View Article and Find Full Text PDFHuman metapneumovirus (HMPV) shares clinical and epidemiological characteristics with well-known respiratory syncytial virus (RSV). The aim of this study was to investigate the clinical and epidemiological differences between HMPV- and RSV-induced wheezing illnesses. A total of 1,008 nasopharyngeal aspirate specimens was collected from 1,008 pediatric patients hospitalized with acute respiratory tract infection at Inje University Sanggye Paik Hospital from December 2003 to April 2008, and tested for seven common respiratory viruses.
View Article and Find Full Text PDFObjective: To investigate the effect of montelukast on eosinophil degranulation and recurrent wheezing episodes in post-respiratory syncytial virus (RSV) bronchiolitis.
Study Design: Two hundred infants (age, 6-24 months) who were hospitalized with their first episode of acute RSV bronchiolitis were randomized in a double-blind, placebo-controlled, parallel comparison of 4-mg montelukast granules (RSV-MONT group) or matching placebo (RSV-PLC group) administered for 3 months. Serum eosinophil-derived neurotoxin (EDN) levels were measured (primary outcome), and recurrent wheezing was documented (secondary outcome) for 12 months.
Article Synopsis
- This study evaluates the new VIDAS C. difficile Toxin A & B assay (CDAB) against the existing VIDAS C. difficile Toxin A II assay (CDA) for diagnosing C. difficile infections in areas where TcdA is not present but TcdB is prevalent.
- Out of 555 fecal samples, 150 were positive for C. difficile, showing an 81.8% concordance between the two assays, with the CDAB demonstrating significantly higher sensitivity (65.3%) compared to CDA (29.8%).
- The CDAB assay was more effective at detecting both toxin A and B strains, identifying a greater percentage of the tcdA(+)tcdB(+) and
Enzyme immunoassays (EIA) to detect glutamate dehydrogenase or toxins A (TcdA) and B (TcdB), a cytotoxicity assay, and bacteriologic culture have disadvantages when applied individually to diagnosis of Clostridium difficile infections. Stool specimens (n = 1,596) were subjected to toxin detection via an enzyme-linked fluorescent immunoassay (ELFA; Vidas CDAB assay) and bacteriologic culture for toxigenic C. difficile in a three-step algorithm with additional toxigenic culture.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates how different storage conditions affect the accuracy of the fecal occult blood test (FOBT) used for colorectal cancer screening in adults aged 50 and older.
- It found that specimens stored in buffer-free containers at room temperature showed a significant rate of false negatives, with over half turning negative after just 48 hours.
- Using buffer-tubes significantly reduced false negatives, indicating that proper specimen handling and education for patients is crucial for accurate test results.
Background: The Korean Laboratory Accreditation Program (KLAP) by the Korean Society of Laboratory Medicine (KSLM) was started in 1999. We summarized history and achievement of KLAP for the last 8 yr.
Methods: We analyzed 8 yr data (1999-2006) of historical events, trends of participating laboratories, and scores according to the impact of the question to the outcome of the tests.
Article Synopsis
- Enzyme immunoassays (EIAs) for detecting toxins A and B of Clostridium difficile are evaluated to improve diagnosis of associated diseases.
- A study analyzed 228 stool samples using two different EIAs (ELFA and ELISA) and confirmed findings through cultures and PCR testing on a subset of samples.
- Results showed similar effectiveness between the two EIAs, with ELISA showing slightly better sensitivity and ELFA slightly better specificity; further testing like cultures and PCR is advised if both EIAs return negative.
Background: In previous studies, most hepatitis A virus (HAV) isolates had been genotype IA in Korea. Recently, a small number of different genotypes were reported with an upsurge of acute hepatitis by HAV. We investigated the distribution of HAV genotypes.
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