Metabolic reprogramming and machine learning-guided cofactor engineering to boost nicotinamide mononucleotide production in Escherichia coli.

Nicotinamide mononucleotide (NMN) is a bioactive compound in NAD(P) metabolism, which exhibits diverse pharmaceutical interests. However, enhancing NMN biosynthesis faces the challange of competing with cell growth and disturbing intracellular redox homeostasis. Herein, we boosted NMN production in Escherichia coli by reprogramming central carbon metabolism with a machine learning (ML)-guided cofactor engineering strategy.

TCAD Simulation Study of Electrical Performance of a Novel High-Purity Germanium Drift Detector.

High-purity germanium (HPGe) detectors occupy a prominent position in fields such as radiation detection and aerospace because of their excellent energy resolution and wide detection range. To achieve a broader detection range, conventional HPGe detectors often need to be expanded to cubic-centimeter-scale volumes. However, this increase in volume leads to a large detector area, which in turn increases the detector capacitance, affecting the detector's noise level and performance.

Spatio-temporal evolution and distribution characteristics of the high-quality development of China's insurance industry.

This study, based on the criteria of "inclusiveness," "sustainability," and "internal-external structural coordination," establishes an evaluative framework for appraising the high-quality development of the insurance sector. It systematically gauges the overarching high-quality developmental status of China's insurance industry across regions. Employing non-parametric kernel density estimation, the Standard Deviation Ellipse, and spatial Markov chain, the investigation dynamically scrutinizes the national landscape of high-quality evolution within the insurance sector over the temporal spectrum.

Towards Expressive Spectral-Temporal Graph Neural Networks for Time Series Forecasting.

Time series forecasting has remained a focal point due to its vital applications in sectors such as energy management and transportation planning. Spectral-temporal graph neural network is a promising abstraction underlying most time series forecasting models that are based on graph neural networks (GNNs). However, more is needed to know about the underpinnings of this branch of methods.

HypMix: Hyperbolic Representation Learning for Graphs with Mixed Hierarchical and Non-hierarchical Structures.

Heterogeneous networks contain multiple types of nodes and links, with some link types encapsulating hierarchical structure over entities. Hierarchical relationships can codify information such as subcategories or one entity being subsumed by another and are often used for organizing conceptual knowledge into a tree-structured graph. Hyperbolic embedding models learn node representations in a hyperbolic space suitable for preserving the hierarchical structure.

Interstock-Mediated Graft Incompatibility: Insights into Photosynthetic Pigments, Carbohydrates, Antioxidant Defense Systems, and Hormones Response Mechanisms in Citrus.

Interstock, located between rootstock and scion, plays a critical role in determining graft compatibility. This study aimed to elucidate the physiological mechanisms mediated by interstock in graft compatibility by comparing various leaf and root system parameters between compatible and incompatible graft combinations. These parameters included growth parameters, photosynthetic pigments, carbohydrates, antioxidant enzyme systems, and hormones.

Oxygen Modulation by Ni Metal Sites in Resorcinol-Formaldehyde Resins for Boosted Photocatalytic HO Production From Pure Water.

Utilizing polymeric catalysts to generate hydrogen peroxide (HO) via photocatalysis for various industrial applications is a promising avenue, but the application potential is constrained by their low charge separation efficiency and the capacity for oxygen adsorption and activation. Herein, a polymeric catalyst (NiRF) modified with Ni metal sites on resorcinol-formaldehyde (RF) resin is presented, which can efficiently produce HO through photocatalysis in pure water. The optimal NiRF-5 exhibits an impressive HO generation rate of up to 31.

PAK4 promotes the cytoskeletal organization and meiotic maturation via phosphorylating DDX17 in oocyte.

PAK4 has been widely reported to function in somatic cells. However, its role and the underlying mechanisms in meiotic oocytes are largely unknown. Here, we show that PAK4 deficiency significantly disrupts maturational progression and meiotic apparatus in mouse oocytes.

Striatal neurones expressing D1 dopamine receptors modulate consciousness in sevoflurane but not propofol anaesthesia in mice.

Background: Sevoflurane and propofol are the most widely used inhaled and i.v. general anaesthetics, respectively.

Inhibition of ferroptosis counteracts the advanced maternal age-induced oocyte deterioration.

Ferroptosis, a recently discovered form of programmed cell death triggered by the excessive accumulation of iron-dependent lipid peroxidation products, plays a critical role in the development of various diseases. However, whether it is involved in the age-related decline in oocyte quality remains unexplored. Here, we took advantage of nano-proteomics to uncover that reduced ferritin heavy chain (Fth1) level is a major cause leading to the occurrence of ferroptosis in aged oocytes.

Transglutaminase 2 reduces the polyspermy of porcine oocytes by promoting the hardening of zona pellucida.

Polyspermy is a type of abnormal fertilization that would increase the risk of embryonic death and hence reduced female fecundity. To avoid this occurrence, mammalian oocytes develop the complicated mechanisms of blocks to polyspermy. Our previous study discovered that transglutaminase 2 (Tgm2) is a newly identified molecule responsible for the post-fertilization block to sperm penetration through zona pellucida (ZP) and polyspermy in mice.

NeOPT: neural optical projection tomography with low-cost imaging setup.

Optical projection tomography (OPT) is an imaging technology that employs light to reconstruct 3D models of objects. Recent advancements in neural radiance field (NeRF) have improved 3D biomedical imaging, yet they perform suboptimally with low signal-to-noise ratio (SNR) data in low-cost setups and are not directly applicable to OPT systems. Here, we introduce neural optical projection tomography (NeOPT), leveraging a low-cost OPT imaging setup combined with a pose-robust neural-based 3D reconstruction method to achieve high-quality OPT results.

Comparative Transcriptome Analysis Reveals Potential Molecular Regulation of Organic Acid Metabolism During Fruit Development in Late-Maturing Hybrid Citrus Varieties.

Late-maturing hybrid citrus is a significant fruit that combines the best traits of both parents and is highly prized for its unique flavor. Not only can organic acids alter the flavor of citrus pulp, but they are also essential for cellular metabolism, energy conversion, and maintaining the acidbase balance in plant tissues. Although organic acids play a key role in the quality formation of citrus fruits, there is still insufficient research on the metabolic processes of organic acids in late-maturing hybrid citrus varieties.

Utilizing the renal and vertebral contours as landmarks for right adrenal vein localization in primary aldosteronism: a retrospective analysis of 310 cases.

Background: Adrenal Vein Sampling (AVS) is the gold standard for categorizing primary aldosteronism (PA). However, catheterization of the right adrenal vein (RAV) can be technically challenging. This study aimed to investigate the validity of the right renal vertebral contour as fluoroscopic landmarks to help RAV orifice localization during AVS.

Maternal ELL3 loss-of-function leads to oocyte aneuploidy and early miscarriage.

Up to an estimated 10% of women experience miscarriage in their lifetimes. Embryonic aneuploidy is a leading cause for miscarriage, infertility and congenital defects. Here we identify variants of ELL3, a gene encoding a transcription elongation factor, in couples who experienced consecutive early miscarriages due to embryonic aneuploidy.

Rare and common genetic variants underlying the risk of Hirschsprung's disease.

Hirschsprung's disease (HSCR) is a congenital enteric neuropathic disorder characterized by high heritability (>80%) and polygenic inheritance (>20 genes). The previous genome-wide association studies (GWAS) identified several common variants associated with HSCR and demonstrated increased predictive performance for HSCR risk in Europeans using a genetic risk score, there remains a notable gap in knowledge regarding Chinese populations. We conducted whole exome sequencing in a HSCR case cohort in Chinese.

Mechanisms of breast cancer treatment using Gentiana robusta: evidence from comprehensive bioinformatics investigation.

This study investigates the potential treatment of breast cancer utilizing Gentiana robusta King ex Hook. f. (QJ) through an integrated approach involving network pharmacology, molecular docking, and molecular dynamics simulation.

Does living longer mean living healthier? A comprehensive analysis of 204 countries and regions from 1990 to 2019.

Background: The data from the WHO showed that life expectancy (LE) and health-adjusted life expectancy (HALE) at global level both increased from 2000 to 2019, while they did not increase at the same rate. Therefore, our study aims to explore the relationship and changing trends between LE and HALE, and further comprehensively analyze the factors influencing the difference between the two.

Methods: This paper uses data from the Global Burden of Disease (GBD) database in LE and HALE across 204 countries and regions from 1990 to 2019.

Nicotinamide mononucleotide enhances the developmental potential of mouse early embryos exposed to perfluorooctanoic acid.

Perfluorooctanoic acid (PFOA) exposure severely affects the health of animals and humans, including early embryonic development, but the effective approaches to improve the quality of embryos exposed to PFOA have not been explored. Here, we report that nicotinamide mononucleotide (NMN) can be used to attenuate the impairment of mouse early embryos caused by PFOA exposure. We find that NMN supplementation maintains the normal spindle assembly and proper chromosome alignment by restoring the acetylation level of microtubule to enhance the mitotic capacity of embryos at zygotic cleavage stage under PFOA exposure.

INeAT: an artifact-suppressed and resolution-enhanced computed tomography through iterative neural adaptive tomography.

Computed tomography (CT) with its remarkable capability for three-dimensional imaging from multiple projections, enjoys a broad range of applications in clinical diagnosis, scientific observation, and industrial detection. Neural adaptive tomography (NeAT) is a recently proposed 3D rendering method based on neural radiance field for CT, and it demonstrates superior performance compared to traditional methods. However, it still faces challenges when dealing with the substantial perturbations and pose shifts encountered in CT scanning processes.

Gentiopicroside-Induced gastric cancer necroptosis via the HIF-1 signaling pathway: A study involving molecular docking and experimental validation.

Objectives: Gentiopicroside is an effective treatment for several types of cancer, inducing numerous forms of programmed cancer cell death. However, there are few investigations into the role of necroptosis. By utilizing molecular docking, and experimental validation, this study aims to investigate whether gentiopicroside elicits necroptosis in gastric cancer.

Engineering Exopolysaccharide Biosynthesis of to Promote Electroactive Biofilm Formation for Liquor Wastewater Treatment.

Microbial electrochemical systems (MESs), as a green and sustainable technology, can decompose organics in wastewater to recover bioelectricity. Electroactive biofilms, a microbial community structure encased in a self-produced matrix, play a decisive role in determining the efficiency of MESs. However, as an essential component of the biofilm matrix, the role of exopolysaccharides in electroactive biofilm formation and their influence on extracellular electron transfer (EET) have been rarely studied.

Residual Syntax score and percutaneous coronary intervention in diabetic patients with renal insufficiency.

Objective: To investigate the correlation between residual Syntax score (rSS) and long-term prognosis in diabetic patients with renal insufficiency undergoing percutaneous coronary intervention (PCI).

Methods: In this retrospective study, we included 510 patients with coronary heart disease, diabetes, and renal insufficiency who received PCI at the Third People's Hospital of Chengdu from July 2018 to December 2020. Patients were divided into three groups based on their eGFR levels: 113 patients with eGFR ≥ 60 mL/min/1.

Protective role of nicotinamide mononucleotide in porcine oocyte maturation and fertilization against sodium metabisulfite-induced oxidative stress and apoptosis.

Sodium metabisulfite (SMB) is commonly utilized in food to preserve freshness by acting as an antioxidant and antimicrobial agent. However, when SMB interacts with water in biological systems, it produces harmful byproducts such as sulfite and sulfur dioxide. These byproducts induce cellular stress and programmed cell death, known as apoptosis, by triggering excessive production of reactive oxygen species.