[Effect of polyethylene terephthalate (Dacron®) on functional potential of human bone-marrow/progenitor stem cells].

Vascular grafts made of polytetrafluoroethylene and polyethylene terephthalate have widely been used in cardiovascular surgery. The causes of delayed colonization of such grafts by endotheliocytes and mesenchymal stem cells have not been adequately investigated. The authors examined the effect of polyethylene terephthalate on the functional activity of human bone marrow mesenchymal stem cells and endothelial progenitor cells in vitro.

Glioblastoma-arteriovenous fistula complex: imaging characteristics and treatment considerations.

Although a certain degree of arteriovenous shunting may be expected in glioblastoma, to our knowledge, the coexistence of a glioblastoma and arteriovenous fistula has not been previously reported. In this case report, we present such a lesion and discuss its diagnosis with a multimodal imaging approach. Additionally, we discuss treatment considerations for such a lesion.

Acute calcific longus colli tendinitis: an unusual location and presentation.

Acute calcific longus colli tendinitis (LCT) has been reported as an unusual cause of acute-onset neck pain, dysphagia, and headache.(1-5) As described in most of the published reports, LCT traditionally manifests on computed tomography (CT) imaging as paramidline calcium hydroxyapatite crystal deposits anterior to the C1 and C2 vertebral bodies. However, recent studies have brought attention to the disease existing at the C4-C5 and C5-C6 levels.

Semiautomated detection of cerebral microbleeds in magnetic resonance images.

Cerebral microbleeds (CMBs) are increasingly being recognized as an important biomarker for neurovascular diseases. So far, all attempts to count and quantify them have relied on manual methods that are time-consuming and can be inconsistent. A technique is presented that semiautomatically identifies CMBs in susceptibility weighted images (SWI).

Imaging cerebral microbleeds using susceptibility weighted imaging: one step toward detecting vascular dementia.

Purpose: To monitor changes in the number of cerebral microbleeds (CMBs) in a longitudinal study of healthy controls (HC) and mild-cognitively impaired (MCI) patients using susceptibility weighted imaging (SWI).

Materials And Methods: SWI was used to image 28 HC and 75 MCI patients annually at 1.5 Tesla over a 4-year period.

Characterizing the mesencephalon using susceptibility-weighted imaging.

Background And Purpose: The mesencephalon is involved in a number of human neurodegenerative disorders and has been typically imaged with T1-, T2- and T2*-weighted methods. Our aim was to collect high-contrast susceptibility-weighted imaging (SWI) data to differentiate among and within the basic mesencephalic structures: namely, the red nucleus, substantia nigra, and crus cerebri.

Materials And Methods: High-resolution SWI, 3D T1-weighted, and T2-weighted data were collected to study contrast in the mesencephalon at 1.

[The immunological prognosis of complications in patients undergoing reconstructive operations after the radiation therapy of malignant tumors of the maxillofacial area and neck].

Immunologic studies were carried out in 55 patients with local malignant processes in the maxillofacial area and neck before and after surgery. The patients were divided into 2 groups: group 1 (N = 20) received no specific therapy before surgery, group 2 (n = 35) were administered radiotherapy in a total focal dose of 56-65 Gy before the operation. The findings evidence that radiotherapy augments B and T system immunodeficiency manifestations after surgical stress.

[Ultrastructural changes in the erythrocytes irradiated with ultraviolet light].

The ultrastructure of erythrocytes of donor blood and blood of patients suffering from kidney insufficiency was studied with the help of scanning and transmission electron microscopes before and after it was treated with ultraviolet light. It was concluded that after being treated with ultraviolet light, the ultrastructure of donor blood erythrocytes undergoes significant positive changes which are being characterized by normalization of the form of cells, decrease of associative ties between them, the cytoplasm content becomes more homogenous. There was a noticeable increase of a number of discocytes and a decreased number of associative ties between the cells after the patients' own blood was treated with ultraviolet light.

[Dynamics od reparative processes in bronchial stump after pneumonectomy in experimental animals].

Repair processes in the bronchus stump after pneumonectomy in 25 rats and 25 guinea-pigs were analysed. The tests were conducted on 1, 3, 7, 14 and 30 days of the postoperative period using bronchus stump and its bottom material for semithin ++ slices and electron microscopy, respectively. At early stages, besides necrotic and necrobiotic changes in the bronchus stump, compensatory and adaptive reactions are also observed.

[Dynamics of morphological changes in the epithelial layer of the respiratory tract after endobronchial treatment of suppurative endobronchitis by helium-neon laser irradiation (experimental study)].

The results of a dynamic examination with the use of an electron microscope of semi- and ultrathin sections of the epithelial lining of the respiratory tract in the experimental dogs under endobronchial treatment of nonspecific endobronchitis with varying doses of low-energy radiation from helium-neon laser are presented. It was proved that the outcomes of regeneration of the bronchial mucosal membrane epithelium under endobronchial treatment of purulent endobronchitis with low-energy radiation of the helium-neon laser much depend both on exposure time (single doses of energy), and multiplicity (cumulative dose of energy), and the period after the laser therapy. The most favourable regeneration takes place under 5-minute (single dose of 4.

[The effect of carnosine on the healing of a lung wound].

The influence of carnosine (beta-alanine, alpha-histidine) on the process of lung wound reparation was studied in 90 guinea-pigs. Its efficacy was evaluated microscopically, histologically and by means of electronic microscope. It was established that carnosine, as compared to controls nearly twice accelerates reparative processes in the injured lung by activation of fibroblast proliferation, connective tissue generation and intracellular regeneration.

[Experimental regeneration of the lungs].

In experiments on 150 guinea pigs, processes of lung wound healing were followed and the more active cellular sources of regeneration were identified. Measurements of cyclic adenosine monophosphate components in the course of lung healing indicated that histologic and electron-microscopic studies would be more informative if done at days 1, 3, 4, 7, and 14 after lung damage. Both cellular and intracellular mechanisms of regeneration were involved in the healing processes.

[Changes in the respiratory organs in experimental adenovirus infection].

An experiment on 6 green monkeys and on 286 cotton newly born rats was made with the aim of studying the lung during experimental adenovirus infection. All the animals during different terms of infection (from 6 hours and up to 40 days) have been studied. Several morphological changes were discovered in the lungs of monkeys and rats after 6 hours of infection and were retained up to the 10-th day of infection.

[Variants of the ultrastructure of bronchial ciliary cells].

The structure of the ciliated epithelium and its regeneration under normal conditions were studied using biopsy specimens of the trachea and extrapulmonary bronchi from healthy dogs. The specimens were processed with the routine methods for electron microscopy. Along with the typical structure of the ciliated cells there were observed various deviations considered earlier as consequences of bronchopulmonary pathology: changed cytoplasm of the ciliated cells in the form of cytoplasmic processes or follicles.

[Treatment of nonspecific endobronchitis with low-intensity laser irradiation (experimental study)].

Results of endobronchial treatment of experimental purulent endobronchitis (PE) in dogs with low intensity He-Ne laser are presented. The endobronchial use of the laser energy in treatment of PE was shown expedient and valid. Comparison of the visual endoscopic picture with the findings of the histochemical and electron microscopic examination of the bronchial mucosa bioptates in the time course of the treatment provided establishment of the optimal regimen for the treatment of nonspecific PE with He-Ne laser.

[The effect of ultraviolet irradiation of patients' blood on the morphofunctional status of erythrocytes].

The morphofunctional status of red blood cells was studied in patients with terminal renal failure associated with pyo-inflammatory complications treated by means of ultraviolet radiation of autologous blood. The authors have established that ultraviolet radiation of autologous blood results in the increase of the discocyte count and red blood cell activation, evidenced by the rise of the young red blood cell count, of the red blood cell population with marked activity of glucose 6-phosphate dehydrogenase, of the content of sulfhydryl groups, and by the increase of erythrocytic adhesion.

[Morphofunctional changes in blood cells irradiated with ultraviolet light].

Red cell and leukocyte morphology and function have been examined in donor blood UV irradiated in a flow closed circulation. The studies have revealed that a single UV irradiation enhances red cell resistance to chemical hemolysis, helps them acquire normal shape; besides, it improves leukocytic functional activity, that manifests by enhanced phosphatase activity and augmentation of the granulocyte phagocytic activity.

[Regeneration of the alveolar tree after suppurative-destructive diseases of the lungs].

Electron microscopic examinations of the lungs of animals with the model of experimental staphylococcal destruction of the lungs have revealed the regeneration of lung tissues which took place by the 6th month from the onset of the disease. The stimulating effect of laser radiation on the cell growth at the expense of mitosis was observed in the culture of epithelioid cells of the lung tissue. Findings of bronchography, angiopulmonography and an analysis of the function of external respiration in 10 patients have shown a complete anatomical and functional recovery of the alveolar tissue in sites of the former localization of pulmonary abscesses.

[Characteristics of development of the rat embryonal lung after resection of the maternal lung].

Histological and electron microscopic studies of embryos from pulmonectomized female rats have revealed retarded differentiation of respiratory rudiment epithelium manifested in delayed appearance of osmiophylic laminar corpuscles and decreased number of type II pneumocytes in respiratory rudiment lining. Osmiophylic laminar corpuscles in them were less differentiated, as compared to the control. In addition, embryonic lungs in pulmonectomized rats were characterized by excessive mesenchyma development and respective decrease in respiratory rudiment area.