High throughput pH bioassay demonstrates pH adaptation of Rhizobium strains isolated from the nodules of Trifolium subterraneum and T. repens.

The purpose of developing this high throughput assay was to determine whether there was evidence of pH adaptation in strains of rhizobia which nodulate subterranean clover (SC) and white clover (WC), and whether this was related to the pH of the soil of origin. pH is a first-order factor influencing the niche preferences of soil microorganisms and has been convincingly shown to be a key driver of soil bacterial communities. Naturalised strains of Rhizobium spp.

Trifolium repens and T. subterraneum modify their nodule microbiome in response to soil pH.

Aims: The influence of soil edaphic factors on recruitment and composition of bacteria in the legume nodule is unknown. Typically, low (acidic) pH soils have a negative effect on the plant-rhizobia symbiosis and thereby reduce clover growth. However, the specific relationship between soil pH and the ecology of rhizobia is unknown, in either their free-living or nodule-inhabiting states.

Non-host larvae negatively impact persistence of the entomopathogen Beauveria bassiana in soil.

A better understanding of the ecology of the insect pathogenic fungus, Beauveria bassiana, in soil is needed to identify reasons behind the variable efficacy often seen after field application. A transformed strain of a candidate commercial strain of B. bassiana (F418 gfp tr3), expressing the green fluorescent protein and the hygromycin B resistance gene, was used to assess the effects of the larvae of a host insect, Tenebrio molitor L.

[Towards a partnership-based care approach to adolescence].

The transition from child psychiatry to adult psychiatry is difficult. The need to reinforce this 'bridge' between the services is clear. A system focused on the assessment and orientation of adolescents, set up a year ago by Lille-Métropole public mental health service, aims to favour collaboration on a local level between the different players from the social, medical and educational sector working with young people aged between 12 and 21 in order to facilitate this transition.

Genome sequence of the clover symbiont Rhizobium leguminosarum bv. trifolii strain CC275e.

Rhizobium leguminosarum bv. trifolii strain CC275e is a highly effective, N2-fixing microsymbiont of white clover (Trifolium repens L.).

[Precariousness, DRG's and health planning: pilot study at the Lariboisière hospital in Paris].

Objectives: This paper considers the specific administrative procedures set up by managers of public healthcare establishments and those responsible for health and welfare policies to care for low-income pregnant women for whom 100% of the "price per act" (T2A) is refunded. What are the limitations and what improvements can be suggested?

Patients And Method: The results are based on an analysis of data from semi-structured interviews, legislation and documents.

Results: The State, health insurance systems, public health establishments, local authorities, charities and outpatient services are involved in handling low-income parturients in different services and different establishments, both locally and regionally.

Mapping a candidate gene (MdMYB10) for red flesh and foliage colour in apple.

Background: Integrating plant genomics and classical breeding is a challenge for both plant breeders and molecular biologists. Marker-assisted selection (MAS) is a tool that can be used to accelerate the development of novel apple varieties such as cultivars that have fruit with anthocyanin through to the core. In addition, determining the inheritance of novel alleles, such as the one responsible for red flesh, adds to our understanding of allelic variation.

Development of sexual dimorphism in the suprachiasmatic nucleus of the rat.

Synapses develop at similar rates in the suprachiasmatic nucleus of rats of both sexes, but values are higher in male than in female animals from birth to maturity. Male-type development cannot be mimicked by neonatal androgenization but results suggest that female-type development can be induced by neonatal castration of males. The results suggested that both prenatal and postnatal androgens are essential to normal male development.

Androgen-induced sexual dimorphism in high affinity dopamine binding in the brain transcends the hypothalamic-limbic region.

1 High affinity binding of [3H]-dopamine and [3H]-5-hydroxytryptamine ([3H]-5-HT) was measured in membrane fractions prepared from cerebral cortex, amygdala, hypothalamus, thalamus and brain stem of rats of either sex and of rats which had been either neonatally castrated or androgenized. 2 Binding was measured in rats of 8, 20 and 30 days old as well as in adults. 3 [3H]-dopamine bound with approximately 30 nM affinity ahd [3H]-5-HT with approximately 10 nM affinity to all areas of the brain tested.