Publications by authors named "Bloemendaal F"
Objective: Macrophage interleukin (IL)-10 signalling plays a critical role in the maintenance of a regulatory phenotype that prevents the development of IBD. We have previously found that anti-tumour necrosis factor (TNF) monoclonal antibodies act through Fcγ-receptor (FcγR) signalling to promote repolarisation of proinflammatory intestinal macrophages to a CD206+ regulatory phenotype. The role of IL-10 in anti-TNF-induced macrophage repolarisation has not been examined.
View Article and Find Full Text PDFBackground And Aims: We have recently shown that the mode of action of IgG1 anti-tumour necrosis factor [TNF] antibodies in inflammatory bowel disease [IBD] requires Fcγ-receptor [FcγR] engagement on macrophages. Here we examine the effect of Fcγ-receptor signalling by anti-TNF on macrophage IL-12/IL-23 secretion.
Methods: Cytokine production by human inflammatory macrophages was assessed at the level of RNA and protein.
Article Synopsis
- Regulatory macrophages are crucial for tissue repair, and combining anti-TNF antibodies with thiopurines has shown improved outcomes, but thiopurines have significant side effects.
- Researchers tested 1,600 drug compounds alongside anti-TNF to find safer alternatives for enhancing the induction of regulatory macrophages, using mixed lymphocyte reactions.
- The study identified albendazole as a promising compound that enhances regulatory macrophage induction and improves clinical symptoms in a colitis model, suggesting it could be a safe and effective alternative for combination therapy with anti-TNF in inflammatory bowel disease (IBD).
Background & Aims: Although tumor necrosis factor (TNF) antagonists reduce many clinical features of inflammatory bowel disease, complete mucosal healing occurs in fewer than 50% of patients. The Fc-region of monoclonal antibodies against TNF has immunosuppressive properties via effects on macrophage polarization. We examined the interaction between the anti-TNF Fc-region and Fcγ receptors (FcγR), and whether the absence of the Fc core fucose (which increases binding to FcγRIIIa) increases the efficacy of anti-TNF in mice with colitis.
View Article and Find Full Text PDFBackground And Aims: Anti-tumour necrosis factor [TNF] antibodies induce regulatory macrophages which display a phenotype resembling M2 type macrophages. Anti-TNF induced macrophages [Mϕind] have immunosuppressive and wound healing properties. The factors that contribute to the induction of Mϕind remain to be explored.
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