Tumor promotion by γ and suppression by β non-muscle actin isoforms.

Here we have shown that β-cytoplasmic actin acts as a tumor suppressor, inhibiting cell growth and invasion in vitro and tumor growth in vivo. In contrast, γ-cytoplasmic actin increases the oncogenic potential via ERK1/2, p34-Arc, WAVE2, cofilin1, PP1 and other regulatory proteins. There is a positive feedback loop between γ-actin expression and ERK1/2 activation.

[Pleomorphic fibrosarcoma].

263 tumors diagnosed as malignant fibrous histiocytomas and undifferentiated pleomorphic sarcomas have been reassessed morphologically, immunohistochemically and ultrastructurally. The diagnosis of pleomorphic fibrosarcoma was made in 19 patients (5%). The tumors occur in the lower and upper extremities and trunk.

[Inflammatory fibrosarcoma: a clinical morphological study of six cases].

The report describes the clinical and morphological characteristics of inflammatory fibrosarcoma (6). Tumor occurred in the mediastinum, small pelvis and liver in patients aged 12-64 yrs. It featured infiltrative growth.

[Infantile myofibroma and myofibromatosis: clinical and morphologic assessment of eighteen cases].

Infantile myofibroma (myofibromatosis) (IM) is a mesenchymal tumor of soft tissues of the head, extremities and trunk. It is characterized by relapse-free infiltrative growth. It consists of neoplastic myofibroblasts at varying stage of cell differentiation which form multi-structured tumor zones.

[Sclerosing epithelial fibrosarcoma: a clinico-morphological evaluation of 10 cases].

Sclerosing epithelial fibrosarcoma is a malignant mesenchymal tumor (fibroblastic class). It involved tissues of the legs and trunk in patients aged 31-59 and grew slowly as an infiltrative neoplasm during 6-18 months. Relapse occurred in 20% although no metastases were reported.

[Histogenesis of infantile fibrosarcoma].

Eight cases of infantile deep soft tissue fibrosarcoma were examined. The tumor consists of neoplastic fibroblasts and myofibroblasts which differ from each other ultrastructurally. Well-differentiated collagen-forming neoplastic fibroblasts form one-half of infantile fibrosarcoma.

[Infantile hemangiopericytoma: a clinico-morphological study of 10 cases].

Infantile hemangiopericytoma (IH) is a rare mesenchymal tumor of the extremity and trunk soft tissues with medium potential grade. Its clinical course and prognosis on the basis of morphological evidence are unpredictable. IH consists of CD34-positive fibroblasts forming around fissural vessels.

[Thermodynamic and hydrodynamic study of Bence-Jones proteins].

Four Bence-Jones proteins were investigated by CD, fluorescence and analytical ultracentrifugation methods at physiological conditions (10 mM phosphate buffer, pH 7.0, 100 mM NaCl). A joint analysis of optical melting curves for proteins and their fragments were demonstrated that protein VAD has reduced stability of its constant half, which correlates with the ability of both intact protein and its constant, rather than variable part to form amyloid fibrils.

[Diagnosis of micrometastases of alveolar rhabdomyosarcoma].

Alveolar rhabgomyosarcoma is a highly malignant, small blue cell pediatric soft tissue tumor. Identification of micrometastases in alveolar rhabdomyosarcoma is important because the poor prognosis associated with this subgroup necessitates a modified therapeutic regimen. Since the obtained lymph node specimen can be very small; rhabdomyosarcoma cells are not easily detected using conventional histological methods.

[Low-grade fibromyxoid sarcoma: analysis of 8 cases].

The diagnosis of low-grade fibromyxoid sarcoma was made in 8 patients. The lesions were 2-17 cm in the lower extremities and trunk. One patient had a long history of painless subcutaneous and intramuscular mass (median 1 year).

[Effect of Ca2+ ions on hydrodynamic properties of pentamer and decamer of C-reactive protein in solution].

The molecular mass and sedimentation coefficient of native C-reactive protein in solution were determined by analytical ultracentrifugation in the presence and absence of calcium ions. Pentameric C-reactive protein was shown to be the major macroscopic form of this protein in solution. The removal of calcium ions from solution caused decompaction of the protein accompanied by changes in its hydrodynamic parameters.

[Aggregation of C-reactive protein in solutions at acid pH].

The hydrodynamic properties of the C-reactive protein (CRP) at different pH were studied using quasi-elastic light scattering, size-exclusion liquid chromatography, and nonreducing gel electrophoresis. It was shown that a CRP solution at pH 5.0-7.

[Treatment of burn surfaces by proteinases: mathematical description of an enzyme distribution].

The process of penetration of a proteolytic enzyme applied to the surface of burn wound into the depth of necrotic tissue was considered. The model approximation describes three factors by a series of mathematical equations: inward-directed enzyme diffusion, counter-flow filtration of interstitial fluid (exudates), and irreversible inactivation of the enzyme by specific inhibitors present in exudates. According to the model, a quasi-stationary distribution of enzymatic activity through the thickness of the necrotic layer is achieved within 3 h and persists as long as the enzyme concentration on the wound surface is constant.

[Hydrodynamic parameters of native C-reactive protein molecule in a solution].

The hydrodynamic properties of the C-reactive protein in solution (pH 6.8) were studied using quasi-elastic light scattering and size-exclusion liquid chromatography. It was shown that the solution containing the C-reactive protein represents a polydisperse system.

[Malignant central nervous system tumors are susceptible to specific antibodies].

The binding of circulating specific IgG to glioblastoma cells from brain tumor biopsies was shown using fluorescence conjugate Protein A-FITC and Western blotting. Blood-brain barrier permeability for antitumor antibodies in vivo in glioblastoma patients is suggested.

Combined surgical and immunotherapeutic treatment of patients with fourth stage colon cancer.

Sixty-five patients with the fourth stage colon cancer were subjected to the combined surgical and immunotherapy. The following conclusions are made: (1) surgical elimination of the bulk of tumor mass is a necessary prerequisite for effective immunotherapy; (2) vaccination with autological tumor cells accompanied with bacille bilié de Calmette-Guérin (BCG) as the adjuvant and with interleukin-2 as the immunostimulator effectively prevents metastasizing after successful surgery; (3) the vaccine must necessary contain living tumor cells adequately presenting tumor antigens; and (4) in some cases, immunotherapy causes undesirable autoimmune complications. They can be registered by corresponding inflammation control methods.