CDK11, a splicing-associated kinase regulating gene expression.

The ability of a cell to properly express its genes depends on optimal transcription and splicing. RNA polymerase II (RNAPII) transcribes protein-coding genes and produces pre-mRNAs, which undergo, largely co-transcriptionally, intron excision by the spliceosome complex. Spliceosome activation is a major control step, leading to a catalytically active complex.

Let's come to order: The influence of question order on willingness to register as an organ donor.

Rationale: As over 90% of people who register to be organ donors do so at the Department of Motor Vehicles (DMV), DMVs are perceived as a key context for increasing donor registration rates. Scholars have recently noted that the driver's license application itself, including the placement of the donor registration item relative to other questions, can possibly influence donor registration behavior. The goal of the current study was to experimentally investigate this possibility.

Antagonist activation exercises elicit similar post-activation performance enhancement as agonist activities on throwing performance.

Background: This study aimed to determine the acute effect of agonist and antagonist conditioning activities (CA) on medicine ball throw performance among female softball players.

Methods: Thirteen national-level female softball players (age 22.2 ± 3.

Therapeutic potential of CDK11 in cancer.

Human cyclin-dependent kinases (CDKs) direct the progression of the cell cycle and transcription. They are deregulated in tumours, and despite their involvement in the regulation of basic cellular processes, many CDKs are promising targets for cancer therapy. CDK11 is an essential gene for the growth of many malignancies; however, its primary cellular function has been obscure, and the mode-of-action of OTS964, the first CDK11 inhibitor and antiproliferative compound, has been unknown.

Three-in-1,000 and dynamic norms: A mixed-method investigation of novel appeals for influencing organ donor registration.

The current research investigated two rarely used appeals for increasing organ donor registration-both with the potential to backfire. The three-in-1000 appeal explains that less than one percent of people will die in such a way that their organs can be donated. This appeal could heighten awareness that donor registration is needed, but it can also convey that registering is futile.

CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1.

RNA splicing, the process of intron removal from pre-mRNA, is essential for the regulation of gene expression. It is controlled by the spliceosome, a megadalton RNA-protein complex that assembles de novo on each pre-mRNA intron through an ordered assembly of intermediate complexes. Spliceosome activation is a major control step that requires substantial protein and RNA rearrangements leading to a catalytically active complex.

The effect of breathing technique on sticking region during maximal bench press.

The intrathoracic pressure and breathing strategy on bench press (BP) performance is highly discussed in strength competition practice. Therefore, the purpose of this study was to analyze whether different breathing techniques can influence the time and track characteristics of the sticking region (SR) during the 1RM BP exercise. 24 healthy, male adults (age 23 ± 2.

Organ donation and departments of motor vehicles: Multiple messages, implementations, and replications.

Objective: To reduce uncertainty about the effectiveness of Departments of Motor Vehicles (DMV) interventions, we assessed the influence of several DMV-based media campaigns on organ donor registration.

Method: Across 4 studies, campaigns were implemented in 8 (Studies 1-3) or 9 (Study 4) New Mexico DMVs. Three theories guided message development (i.

CDK11 is required for transcription of replication-dependent histone genes.

Replication-dependent histones (RDH) are required for packaging of newly synthetized DNA into nucleosomes during the S phase when their expression is highly upregulated. However, the mechanisms of this upregulation in metazoan cells remain poorly understood. Using iCLIP and ChIP-seq, we found that human cyclin-dependent kinase 11 (CDK11) associates with RNA and chromatin of RDH genes primarily in the S phase.

CDK12: cellular functions and therapeutic potential of versatile player in cancer.

Cyclin-dependent kinase 12 (CDK12) phosphorylates the C-terminal domain of RNA polymerase II and is needed for the optimal transcription elongation and translation of a subset of human protein-coding genes. The kinase has a pleiotropic effect on the maintenance of genome stability, and its inactivation in prostate and ovarian tumours results in focal tandem duplications, a CDK12-unique genome instability phenotype. aberrations were found in many other malignancies and have the potential to be used as biomarkers for therapeutic intervention.

Systematic review of intra-abdominal and intrathoracic pressures initiated by the Valsalva manoeuvre during high-intensity resistance exercises.

The Valsalva manoeuvre, intra-abdominal pressure (IAP) and intrathoracic pressure (ITP) play important roles in resistance training and common daily activities. The purpose of this review is to summarize the ITP and IAP responses to resistance exercises and to determine which exercises elicit the highest or lowest body pressure values under high-intensity resistance exercise. The PubMed, Scopus and Web of Science databases were searched until November 1, 2018.

CDK12 controls G1/S progression by regulating RNAPII processivity at core DNA replication genes.

CDK12 is a kinase associated with elongating RNA polymerase II (RNAPII) and is frequently mutated in cancer. CDK12 depletion reduces the expression of homologous recombination (HR) DNA repair genes, but comprehensive insight into its target genes and cellular processes is lacking. We use a chemical genetic approach to inhibit analog-sensitive CDK12, and find that CDK12 kinase activity is required for transcription of core DNA replication genes and thus for G1/S progression.

It's all relative: Increasing organ donor registration intentions by maximizing family-relevant vested interest.

Guided by vested interest theory, the impact of educating potential organ donors about the beneficial experiences afforded to families of deceased donors was assessed. Participants were informed about these benefits by taking a survey that asked them to indicate both their awareness, and the appeal, of numerous existing benefits (e.g.

The Value of Being Discrete: The Differential and Causal Effects of Positive Affect and Discrete Positive Emotions on Organ Donor Registration Outcomes.

Background: The current studies examined how positive affect (i.e. the blend of several positive feelings over time) and discrete positive emotions (i.

The Role of Visual Feedback on Power Output During Intermittent Wingate Testing in Ice Hockey Players.

Visual feedback may help elicit peak performance during different types of strength and power testing, but its effect during the anaerobic Wingate test is unexplored. Therefore, the purpose of this study was to determine the effect of visual feedback on power output during a hockey-specific intermittent Wingate test (AnWT6x6) consisting of 6 stages of 6 s intervals with a 1:1 work-to-rest ratio. Thirty elite college-aged hockey players performed the AnWT6x6 with either constant ( = 15) visual feedback during all 6 stages (CVF) or restricted ( = 15) visual feedback (RVF) where feedback was shown only during the 2nd through 5th stages.

A systematic review of surface electromyography analyses of the bench press movement task.

Background: The bench press exercise (BP) plays an important role in recreational and professional training, in which muscle activity is an important multifactorial phenomenon. The objective of this paper is to systematically review electromyography (EMG) studies performed on the barbell BP exercise to answer the following research questions: Which muscles show the greatest activity during the flat BP? Which changes in muscle activity are related to specific conditions under which the BP movement is performed?

Strategy: PubMed, Scopus, Web of Science and Cochrane Central Register of Controlled Trials (CENTRAL) in the Cochrane Library were searched through June 10, 2016. A combination of the following search terms was used: bench press, chest press, board press, test, measure, assessment, dynamometer, kinematics and biomechanics.

Instillation Therapy for the Treatment of Necrotizing Fasciitis: A Case Study.

A 57-year-old obese female with uncontrolled diabetes mellitus type I presented to the emergency department (ED) with the main complaint of worsening pain and redness in her right groin and inguinal regions. In the ED, a CT scan confirmed the likely presence of necrotizing fasciitis in the right groin and thigh. She was also found to be febrile and septic on admission.

Structural and Functional Analysis of the Cdk13/Cyclin K Complex.

Cyclin-dependent kinases regulate the cell cycle and transcription in higher eukaryotes. We have determined the crystal structure of the transcription kinase Cdk13 and its Cyclin K subunit at 2.0 Å resolution.

The CD8+ cell non-cytotoxic antiviral response affects RNA polymerase II-mediated human immunodeficiency virus transcription in infected CD4+ cells.

A CD8+ cell non-cytotoxic antiviral response (CNAR), mediated by a CD8+ cell antiviral factor (CAF), is associated with a long-term healthy state in human immunodeficiency virus (HIV) infection. CNAR/CAF reduces viral transcription without a known effect on specific viral sequences in the HIV genome. In studies to define the mechanism involved in the block in viral transcription, we now report that transcription from the HIV-LTR reporter is reduced in infected CD4+ cells upon treatment with CAF.

Surface plasmon polaritons at linearly graded semiconductor interfaces.

New results are reported on investigation of dispersion curves for surface plasmon polaritons (SPPs) at an inhomogenously doped semiconductor/dielectric interface whereby the dielectric is represented by the same undoped semiconductor. The doped semiconductor is described by its frequency-dependent permittivity that varies with the depth. It is shown that a transition layer (TL) with a linear change in carrier concentration supports one branch dispersion curve regardless of the TL thickness.

Ovarian carcinoma CDK12 mutations misregulate expression of DNA repair genes via deficient formation and function of the Cdk12/CycK complex.

The Cdk12/CycK complex promotes expression of a subset of RNA polymerase II genes, including those of the DNA damage response. CDK12 is among only nine genes with recurrent somatic mutations in high-grade serous ovarian carcinoma. However, the influence of these mutations on the Cdk12/CycK complex and their link to cancerogenesis remain ill-defined.

Upregulation of CYP1B1 expression by inflammatory cytokines is mediated by the p38 MAP kinase signal transduction pathway.

Cytochrome P450 1B1 (CYP1B1) is an enzyme that has a unique tumor-specific pattern of expression and is capable of bioactivating a wide range of carcinogenic compounds. We have reported previously that coordinated upregulation of CYP1B1 by inflammatory cytokines, such as tumor necrosis factor-α (TNF-α) and the aryl hydrocarbon receptor ligands, may increase bioactivation of promutagens, such as benzo[a]pyrene (BaP) in epithelial cells. Here, we extend those studies by describing a novel mechanism participating in the regulation of CYP1B1 expression, which involves activation of the p38 mitogen-activated protein kinase (p38) and mitogen- and stress-activated protein kinase 1 (MSK1).

The sorption of sulfamethazine on soil samples: isotherms and error analysis.

In this paper, batch sorption of sulfamethazine on eight soil samples (six from Croatia and two from Bosnia and Hercegovina) with different organic matter contents ranging from 1.52 to 12.8% was investigated.

The structure and substrate specificity of human Cdk12/Cyclin K.

Phosphorylation of the RNA polymerase II C-terminal domain (CTD) by cyclin-dependent kinases is important for productive transcription. Here we determine the crystal structure of Cdk12/CycK and analyse its requirements for substrate recognition. Active Cdk12/CycK is arranged in an open conformation similar to that of Cdk9/CycT but different from those of cell cycle kinases.