Recombinant Klotho administration after myocardial infarction reduces ischaemic injury and arrhythmias by blocking intracellular calcium mishandling and CaMKII activation.

Ischaemic heart disease (IHD) remains a major cause of death and morbidity. Klotho is a well-known anti-ageing factor with relevant cardioprotective actions, at least when renal dysfunction is present, but its actions are much less known when renal function is preserved. This study investigated Klotho as a biomarker and potential novel treatment of IHD-associated complications after myocardial infarction (MI) under preserved renal function.

Automated Computerized-Based Intervention to Identify Hypomagnesemia in Primary Care Patients With Arrhythmia.

Objectives: Hypomagnesemia early diagnosis and consequently early, timely magnesium supplementation is of utmost benefit, but it often goes underdiagnosed. The objective was to show and monitor an intervention to identify hypomagnesemia in patients with arrhythmia.

Methods: A cross-sectional study was designed in the laboratory.

Support for temporary protection of displaced populations in the EU: A conjoint experiment.

Millions of people were forced to flee Ukraine after Russia's invasion on February 24, 2022, one of the fastest displacements in decades. Citizens' response in EU countries (where most displaced Ukrainians arrived) has been considerably more positive than in past refugee crises. This study investigates several possible drivers of this difference.

Arm elevation involves changes in the whole spine: an exploratory study using EOS imaging.

Background: Few studies have assessed the participation of the spine in arm elevation. The primary aim of this exploratory study was to specify spinal movements during unilateral arm elevation.

Methods: We used an EOS imaging system to assess 2D global posture (Sagittal Vertical Axis [SVA], T1 and T9 tilt and Central Sacral Line [CSL]) and segmental spine curves (C3-C7 in the sagittal plane only, and T1-T6, T7-T12 and L1-L5 in the sagittal and frontal planes) for four different left arm elevation levels: in the sagittal (Sa) plane (30°Sa: reference position, 140°Sa and 180°Sa), and in the scapular (Sc) plane (180°Sc), in ten right-handed asymptomatic participants (5 women; mean age 24.

Evaluation of electrolyzed water to control fungal trunk pathogens in grapevine nurseries.

Background: Grapevine producers demand solutions to control fungal trunk pathogens (FTPs) in nurseries. Adopting integrated strategies combining several control methods has been indicated as the best approach to prevent or reduce infections on grapevine propagation material. In recent years, electrolyzed water (EW) has emerged as a sustainable alternative for disinfection.

Cecal metabolomics of 2 divergently selected rabbit lines revealed microbial mechanisms correlated to intramuscular fat deposition.

The gastrointestinal microbiota plays a key role in the host physiology and health through a complex host-microbiota co-metabolism. Metabolites produced by microbial metabolism can travel through the bloodstream to reach distal organs and affect their function, ultimately influencing the development of relevant production traits such as meat quality. Meat quality is a complex trait made up of a number of characteristics and intramuscular fat content (IMF) is considered to be one of the most important parameters.

Electrolyzed Water Treatment for the Control of the Zoonotic Pathogen in Aquaculture: A One Health Perspective.

(Vv) is a bacterial pathogen native to warm and brackish water ecosystems that can cause fatal septicemia (Vv-vibriosis) in humans and various farmed fish species. From a One Health perspective, controlling Vv-vibriosis outbreaks on farms is essential not only for animal but also for human health, as it reduces the risk of Vv transmission to humans. Electrolyzed water (EW) is a sustainable control method, exhibiting transient disinfectant properties due to the formation of hypochlorous acid (HOCl).

Managing laboratory test ordering: a challenge in the new laboratory medicine model.

Introduction: The role of Laboratory Medicine in patient care has evolved in the last decades. The same has occurred to the laboratory model, which has evolved from a traditional model where the laboratory is merely involved in clinical decision-making to a leading model where the laboratory is not only involved but also determines decision-making. The advent of new technologies and automation of processes have enabled laboratory professionals to focus on the first and last phase of the analytical process namely, test ordering and decision-making based on laboratory results.

Association of SARS-CoV-2 immunoserology and vaccination status with myocardial infarction severity and outcome.

Background: The COVID-19 pandemic adversely affected the severity and prognosis of patients with acute myocardial infarction (MI) caused by atherothrombosis (type 1 MI). The effect, if any, of COVID-19 vaccination and natural SARS-CoV2 serologic immunity in these patients is unclear. Our aim was to analyze the association between the severity and outcome of patients with type 1 MI and their previous SARS-CoV2 vaccination and serostatus.

A step forward in the diagnosis of urinary tract infections: from machine learning to clinical practice.

Objectives: Urinary tract infections (UTIs) are common infections within the Emergency Department (ED), causing increased laboratory workloads and unnecessary antibiotics prescriptions. The aim of this study was to improve UTI diagnostics in clinical practice by application of machine learning (ML) models for real-time UTI prediction.

Methods: In a retrospective study, patient information and outcomes from Emergency Department patients, with positive and negative culture results, were used to design models - 'Random Forest' and 'Neural Network' - for the prediction of UTIs.

Correlated Responses to Selection for Intramuscular Fat on the Gut Microbiome in Rabbits.

Intramuscular fat (IMF) content is important for meat production and human health, where the host genetics and its microbiome greatly contribute to its variation. The aim of this study is to describe the consequences of the genetic modification of IMF by selecting the taxonomic composition of the microbiome, using rabbits from the 10th generation of a divergent selection experiment for IMF (high (H) and low (L) lines differ by 3.8 standard deviations).

Perception of patients with retinal pathology on aspects of visual function and their management.

Background And Objective: Age-related macular degeneration (AMD) and diabetic retinopathy (DR) are highly prevalent.

Objective: To explore perceptions of patients with AMD or DR about the impact of the disease and treatment on their daily living activities.

Materials And Methods: Semi-structured interviews with a questionnaire developed from validated patient reported outcomes questionnaires.

Advanced non-squamous NSCLC with no actionable oncogenic driver in Spain: a cross-sectional descriptive analysis of data from the Thoracic Tumor Registry.

Background: Non-small cell lung cancer (NSCLC) accounts for the vast majority of all diagnosed lung cancers. According to their histology, most NSCLCs are considered non-squamous cell carcinoma (NSCC), and up to 85% of the latter may lack either one of the two main actionable oncogenic drivers (i.e.

Clinical trial on nurse training through virtual reality simulation of an operating room: assessing satisfaction and outcomes.

Introduction: Virtual reality (VR) provides a firsthand active learning experience through varying degrees of immersion. The aim of this study is to evaluate the use of VR as a potential tool for training operating room nurses to perform thoracic surgery procedures.

Methods: This is an open parallel-group randomized clinical trial.

Last-Century Forest Dynamics in a Highland Pyrenean National Park and Implications for Conservation.

Ecological records from before and after the creation of natural parks are valuable for informing conservation and management but are often unavailable. High-resolution paleoecological studies may bridge the gap and provide the required information. This paper presents a 20th-century subdecadal reconstruction of vegetation and landscape dynamics in a national park of the Pyrenean highlands.

Microbiome composition as a potential predictor of longevity in rabbits.

Background: Longevity and resilience are two fundamental traits for more sustainable livestock production. These traits are closely related, as resilient animals tend to have longer lifespans. An interesting criterion for increasing longevity in rabbit could be based on the information provided by its gut microbiome.

Laboratory reflex testing strategy for the early identification of primary care patients with multiple myeloma.

Objectives: Our objective was to shorten the screen for multiple myeloma (MM), through reflex testing.

Design And Methods: The clinical laboratory in the public University Hospital of San Juan (Alicante, Spain), serves 234,551 inhabitants. Through an intervention agreed with general practitioners, the Laboratory Information System (LIS) automatically registered serum immunoglobulins (Ig) when serum total proteins (STP) > 80 g/L for the first time in primary care patients.

Double positivity for rheumatoid factor and anti-CCP autoantibodies: improving referral from primary care of patients suspected of having rheumatoid arthritis.

Background: Rheumatoid arthritis (RA) is a chronic progressive autoimmune inflammatory disease with significant morbidity and mortality. The course of the disease can be modified if diagnosis is early and treatment appropriate.

Aim: In this study, we aimed to evaluate a new strategy for early identification of RA patients in primary care settings (the 'diagnostic bottleneck') based on serological biomarkers and to manage inappropriate rheumatoid factor (RF) laboratory test requests.

Circulating Immune Proteins: Improving the Diagnosis and Clinical Outcome in Advanced Non-Small Cell Lung Cancer.

Immunotherapy has been proven a viable treatment option for non-small cell lung cancer (NSCLC) treatment in patients. However, some patients still do not benefit. Finding new predictive biomarkers for immunocheckpoint inhibitor (ICI) response will improve treatment management in the clinical routine.

Clinical Decision Support System in laboratory medicine.

Clinical Decision Support Systems (CDSS) have been implemented in almost all healthcare settings. Laboratory medicine (LM), is one of the most important structured health data stores, but efforts are still needed to clarify the use and scope of these tools, especially in the laboratory setting. The aim is to clarify CDSS concept in LM, in the last decade.

A Continuous Centennial Late Glacial-Early Holocene (15-10 cal kyr BP) Palynological Record from the Iberian Pyrenees and Regional Comparisons.

This paper presents the first continuous (gap-free) Late Glacial-Early Holocene (LGEH) pollen record for the Iberian Pyrenees resolved at centennial resolution. The main aims are (i) to provide a standard chronostratigraphic correlation framework, (ii) to unravel the relationships between vegetation shifts, climatic changes and fire, and (iii) to obtain a regional picture of LGEH vegetation for the Pyrenees and the surrounding lowlands. Seven pollen assemblage zones were obtained and correlated with the stadial/interstadial phases of the Greenland ice cores that serve as a global reference.

Genome-Wide Association Study of Maternal Genetic Effects on Intramuscular Fat and Fatty Acid Composition in Rabbits.

Maternal genetic effects (MGE) could affect meat quality traits such as intramuscular fat (IMF) and its fatty acid composition. However, it has been scarcely studied, especially in rabbits. The objectives of the present study were, first, to assess the importance of MGE on intramuscular fat and fatty acid composition by applying a Bayesian maternal animal model in two rabbit lines divergently selected for IMF.

Reporting age-adjusted D-dimer cut-off values reduces radiology overuse in emergency department patients with suspected deep venous thrombosis.

Introduction: Venous thromboembolism (VTE) is a leading cause of death, associated with substantial morbidity in the absence of treatment. Our aim was, first, to compare the diagnostic performance of D-dimer for the diagnosis of VTE in the emergency department (ED), when reporting conventional cut-off point versus when additionally reporting age-adjusted values. Second, we explored the ordering pattern of Doppler ultrasound (US) and computerized tomographic pulmonary angiogram (CTPA), before and after reporting of the aforementioned age-adjusted cut-off value.