Publications by authors named "Blanca Esparza-Gonsalez"
Selection and Validation of Reference Genes for Gene Expression Studies in an Equine Adipose-Derived Mesenchymal Stem Cell Differentiation Model by Proteome Analysis and Reverse-Transcriptase Quantitative Real-Time PCR.
Genes (Basel)
March 2023
Article Synopsis
- Adipose-derived stem cells (ADSCs) are investigated for their potential in tissue regeneration, specifically through a study focused on establishing stable reference genes for gene expression analysis in equine adipose-derived mesenchymal stem cells (EADMSCs).
- The researchers differentiated ADSCs into adipocytes and osteoblasts and utilized liquid chromatography tandem mass spectrometry to analyze their protein profiles, identifying proteins consistently expressed across all cell types.
- The study validated the mRNA expression stability of the identified genes through RT-qPCR, showing significant increases in specific gene expressions in adipocytes and osteoblasts compared to the original ADSCs, highlighting the importance of using stable reference genes for accurate gene expression normalization.
The aim of this research was to determine the impact of heat stress on cell differentiation in an equine mesenchymal stem cell model (EMSC) through the application of heat stress to primary EMSCs as they progressed through the cell specialization process. A proteomic analysis was performed using mass spectrometry to compare relative protein abundances among the proteomes of three cell types: progenitor EMSCs and differentiated osteoblasts and adipocytes, maintained at 37 °C and 42 °C during the process of cell differentiation. A cell-type and temperature-specific response to heat stress was observed, and many of the specific differentially expressed proteins were involved in cell-signaling pathways such as Notch and Wnt signaling, which are known to regulate cellular development.
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