A tumor promoter stimulates phosphorylation on tyrosine.

The tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate is mitogenic for normal chicken embryo fibroblasts and also causes these cells to express transiently many properties of cells transformed by Rous sarcoma virus. Since some mitogenic hormones stimulate a tyrosine-specific protein kinase activity, and since the transforming protein of RSV is a tyrosine-specific protein kinase, we have examined whether TPA also stimulates protein phosphorylation on tyrosine. We report here that TPA treatment of normal cells resulted in a very rapid phosphorylation on tyrosine of a protein peak of Mr 40 to 43 kilodaltons.

Cryptosporidiosis in hospital patients with gastroenteritis.

Among 884 hospital patients with gastroenteritis, 36 (4.1%) were excreting Cryptosporidium oocysts in their stools; only 5 of the 36 patients were also excreting other enteropathogens, while none of 320 hospital patients without gastroenteritis were excreting Cryptosporidium oocysts. Children were more commonly infected with Cryptosporidium (4.

Clinical immunity after neonatal rotavirus infection. A prospective longitudinal study in young children.

To determine whether rotavirus infection in newborn babies conferred immunity to postneonatal rotavirus infection, we studied 81 babies at birth and kept them under clinical and serologic study for three years. During the first 14 days of life, 44 of the infants excreted rotavirus, and 37 did not. Fifty-five per cent of those with neonatal infection and 54 per cent of those without it had rotavirus infection during the next three years.

The BPRS in assessing symptom correlates of cerebral ventricular enlargement in acute and chronic schizophrenia.

The present study was an attempt to investigate hypotheses about the interrelationship of brain dysfunction and symptoms of schizophrenia using the Brief Psychiatric Rating Scale (BPRS) and a measure of cerebral ventricular size. The ventricular brain ratio (VBR) was correlated with admission and discharge scores on the BPRS in 46 schizophrenic patients. A significant relationship was found between VBR and discharge BPRS scores.

Exercise-induced ST segment alternans.

We report a rare electrocardiographic finding occurring in previously undescribed circumstances in which a 61-year-old man undergoing exercise testing developed striking ST segment elevation on the ECG characterized by electrical alternans of the ST segments. The significance of electrical alternans is briefly discussed in the light of this event.

A gas chromatographic procedure for the determination of airborne MDI and TDI.

A method is described for the analysis of airborne diphenylmethane diisocyanate (MDI) and toluene diisocyanate (TDI) using gas-liquid chromatography. The diisocyanates are collected in acidic absorbing solution where they undergo hydrolysis, converted to the free diamines with caustic, and extracted into toluene. The diamines are derivatized in the toluene with heptafluorobutyric anhydride (HFBA).

Ventricular enlargement in major depression.

Major depression accompanied by psychosis may be a separate nosological entity from nonpsychotic depression. Investigators have noted behavioral and biochemical differences in psychotic and nonpsychotic patients, as well as differences in response to treatment. A previous study using computed tomography (CT) found enlargement of the lateral cerebral ventricles in patients with manic-depressive illness with psychotic symptoms.

Epidemiology of rotavirus diarrhea in the Highlands of Papua, New Guinea, in 1979, as revealed by electrophoresis of genome RNA.

Results of gel electrophoresis of rotavirus genome RNA from feces of children in two provinces in Papua, New Guinea, suggest that the epidemiology of rotavirus infection in small communities with a total population of 3,000 may differ from that in urban or closely settled rural areas.

Effect of oral propranolol on left ventricular performance in patients with ischemic heart disease. Ear densitographic study during uninterrupted treadmill exercise.

The effect of oral propranolol on left ventricular performance during early upright exercise was evaluated by ear densitography in patients with arteriographic coronary artery disease (CAD). Measurements of systolic time intervals differentiated 10 unmedicated patients with CAD (group 1) and 15 patients with CAD taking propranolol (group 3). The patients in group 3 had less shortening of preejection period at 1 minute and 4 minutes of exercise than group 1 patients (p less than 0.

Development of gut colonisation in pre-term neonates.

Twenty-eight pre-term babies of low birth weight were monitored for developing microflora in throat, stomach and faeces during the first 3 weeks of life. The flora at all levels of the gastrointestinal tract differed from that of healthy breast-fed and artificially fed full-term babies. Colonisation of throat and stomach was delayed beyond 4 days of life in 87% and 60% of babies respectively.

Epidemiology of rotavirus diarrhea in Yogyakarta, Indonesia, as revealed by electrophoresis of genome RNA.

Nine different electropherotypes of rotaviruses occurred among 85 children with rotavirus diarrhea in Yogyakarta, Indonesia. Eighty percent of the electropherotypes had a "long" RNA pattern characteristic of human subgroup 2.

Detection of a rotavirus-like agent associated with diarrhea in an infant.

A rotavirus-like agent was detected in the feces of a child with diarrhea. Although morphologically indistinguishable from rotavirus, the agent was serologically distinct, and electrophoresis of its nucleic acid also showed that it was dissimilar.

Sudden death recorded during Holter monitoring.

Six instances of sudden death were recorded by Holter monitoring, which showed ventricular fibrillation in five and a bradyarrhythmia in one. Complex ventricular ectopic activity preceded cardiac arrest in five patients, including the one with the bradyarrhythmic arrest. Two patients with chronic bifascicular block arrested as a result of ventricular fibrillation.

A randomised trial of oral gammaglobulin in low-birth-weight infants infected with rotavirus.

Oral human gammaglobulin or placebo was given with each feed during the first week of life to 75 low-birth-weight babies. All were in a nursery where rotavirus was known to be endemic, 25 of the babies excreted rotavirus during the first 2 weeks of life. This group was regarded as the "challenge" group.

Acute diarrhea and rotavirus infection in newborn babies and children in Yogyakarta, Indonesia, from June 1978 to June 1979.

A longitudinal study of acute diarrhea in children in Yogyakarta, Indonesia (June 1978 to June 1979), showed little variation throughout most months of the year in numbers of children admitted to hospital and in numbers infected with rotaviruses. Both decreased during November and December coincidentally with seasonal change from dry to wet conditions. Rotavirus particles were identified by electron microscopy in fecal specimens from 126 of 334 (38%) infants and children with acute diarrhea.

Noninvasive assessment of left ventricular performance in patients with ischemic heart disease: ear densitographic study during uninterrupted treadmill exercise.

Among 738 consecutive patients with chest pain referred for exercise testing, only 17 men fulfilled strict criteria for this pilot study: (1) achievement of at least 90 percent of age-predicted maximal heart rate or electrocardiographic criteria for a positive test; (2) subsequent coronary angiography; (3) exclusion of prior heart disease; and (4) absence of medication. Measurements of systolic time intervals obtained with ear densitography during early exercise differentiated 10 patients with coronary artery disease (Group 1) from 17 men (7 patients plus 10 normal volunteers) with normal coronary arteries (Group 2). Despite nearly identical heart rate and blood pressure responses in both groups, men in Group 1 had a significantly greater reduction of preejection period (PEP) at 1 minute and 4 minutes as well as a greater decrease in PEP/LVET (left ventricular ejection time) ratio.

Midsystolic closure of the aortic valve in primary pulmonary hypertension.

Echocardiographic examination in a patient with primary pulmonary hypertension demonstrated midsystolic closure of the aortic valve, which has often been described in hypertrophic cardiomyopathy with or without obstruction, in discrete subaortic stenosis, and in ruptured aneurysm of the right coronary sinus of Valsalva. A possible mechanism for this finding in primary pulmonary hypertension and differential diagnosis from other diseases are discussed.

Elucidation of physiologic lengthening of left ventricular ejection time during early upright exercise.

To elucidate the relationship between heart rate (HR) and left ventricular ejection time (LVET) during early exercise, 30 patients with chest pain were studied at 1 (1') and 4 minutes (4'). Mean results for control leads to 1' exercise: HR 79 to 105 beats per minute, LVET 247 to 260 msec. Thus instead of shortening as predicted by the HR change at 1' of exercise, LVET rose significantly (p less than 0.