Characterization of the sat4 gene encoding a streptothricin acetyltransferase in Campylobacter coli BE/G4.

The sat4 streptothricin resistance gene from Campylobacter coli BE/G4 was cloned into pUC18, and its nucleotide sequence was determined. Streptothricin acetyltransferase activity was detected in Escherichia coli cells containing recombinant plasmid pAT132 which carries the sat4 gene as an insert. The deduced amino acid sequence displayed 21-27% amino acid identity with streptothricin acetyltransferases from E.

Biosynthesis and characterization of (S)-and (R)-3-hydroxy-3-methylglutaryl coenzyme A.

(S)-3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA), the physiologic substrate of HMG-CoA reductase and of HMG-CoA lyase, is available commercially only as (R,S)-HMG-CoA, a mixture of diastereomers. To provide (S)-HMG-CoA for our continuing investigation of HMG-CoA reductase, we used homogeneous, overexpressed Pseudomonas mevalonii HMG-CoA reductase (EC 1.1.

[Deglutition-induced supraventricular tachycardias].

We describe the cases of two patients who exhibited a supraventricular tachycardia induced by swallowing. In both cases we found a systemic hypertension, other cardiac or gastroesophagic abnormalities were absent. The electrophysiologic studies demonstrated in the first case a supraventricular tachycardia with 1 : 1 AV-conduction and an origin in the right atrium, in the second case an atrial tachycardia with AV-block originating in the right atrium too.

[Survival time and causes of death in dialysis patients].

Renal dialysis was performed, between 1973 and 1983, in 431 patients in terminal renal failure. Of these, 223 (51.7%) are still in treatment, 21 (4.

Different determination of sinoatrial conduction time (SACT) in man.

Using intracardiac recordings of electrical activity and programmed electrical right atrial stimulation of the human heart, the sinoatrial conduction time (SACT) in the method of Strauss et al. 1973 (SACTc) was calculated in 80 patients with and without disturbances of rhythm and compared to the modified measurement of the SACT in the method reported by Narula et al. in 1978 (SACTN).

A physiological model for the pharmacokinetics of methylene chloride in B6C3F1 mice following i.v. administrations.

A physiologic mathematical model was developed to describe the time course of 14C-methylene chloride (14CH2Cl2) distribution and elimination in mice following single i.v. administrations of 10 and 50 mg/kg.

Is serial iodohippurate renography at the bedside reliable enough to monitor renal transplants?

Renograms were obtained immediately after transplantation and repeated every other day beginning 4 days after renal transplantation. In 31 of 32 biopsy-proved acute rejection crises a flattening of phase II (secretion phase) of the renogram occurred. Of the 32 renogram changes 7 were the only signs of rejection at the time of renography.

[Systolic time intervals in chronic calcifying pancreatitis caused by alcohol abuse].

Systolic time intervals corrected for heart rate were measured non invasively in 12 male patients (32 to 59 years) with chronic calcifying pancreatitis of alcoholic origin and compared with 24 normal subjects without evidence for chronic alcoholism or heart disease. Systolic time intervals (in detail: the time from the beginning of QRS to the first heart sound (QS1), the isovolumic contraction time (IVCT), the total electromechanical systolic interval (QS2c), the pre-ejection period (PEPc), the left ventricular ejection time (LVETc) and the ratio PEPc/LVETc) in patients with chronic calcifying pancreatitis were not different when compared with healthy man. Therefore we conclude, that the amount of alcohol that induced a chronic calcifying pancreatitis was not able to alter systolic time intervals as seen in an alcoholic cardiomyopathy.

[Interatrial communication through the coronary sinus].

An unusual case of an interatrial communication through a defect of the coronary sinus together with coarctation of the aorta is reported. In spite of two cardiac catheterization studies, this diagnosis was missed by two different cardiology departments, and anomalous pulmonary vein connection was suspected instead. This illustrates the preoperative diagnostic difficulties which continue during surgery, when exploration of the right atrium fails to detect the suspected atrial defect.

[Different determination of sinoatrial conduction time in patients with and without sinus node dysfunction].

Using intracardiac recordings of electrical activity and programmed electrical right atrial stimulation of the human heart, the sinoatrial conduction time (SACT) in the method of Strauss et al. 1973 (SACTc) was calculated in 80 patients with and without disturbances of rhythm and compared to the modified measurement of the SACT in the method reported by Narula et al. in 1978 (SACTN).

[Cardiac electrophysiologic effect of various catecholamines and parasympatholytic agents].

Using His-bundle-electrography and programmed atrial stimulation the effects of different catecholamines (dopamine, dobutamine, orciprenaline), parasympatholytics like atropine, ipratropiumbromide and the xanthine derivative aminophylline have been studied on the conduction system of the human heart in 119 patients with and without different disturbances of rhythm. In contrast to dopamine a moderate effect was found by dobutamine (3 or 6 micrograms/kg/min) in patients with sinus node dysfunction and delayed AV-conduction. In comparison it must by pointed out, that orciprenaline has the greatest positive dromotropic and chronotropic effect on sinus and AV-node.

[The effects of atenolol on the exercise symptoms of coronary patients. A double blind study (author's transl)].

In an acute trial on the bicycle ergometer, the beta-receptor blocker atenolol in single morning doses of 50 mg produced a significant reduction in blood pressure, heart rate and ischaemic changes in the ECG. Parallel to this there was an increase in the load resistance of the patients. The majority of the parameters show no further changes at double the dosage.

[Electrophysiologic effects of dopamine on the cardiac conduction system (author's transl)].

The electrophysiologic effects of dopamine (Dop.) (3 microgram; n = 11) 6 microgram/kg/min; n = 14) were studied in 20 patients (5 females, 15 males) with and without different disturbances of rhythm utilizing His-Bundle electrography and right atrial pacing. The blood pressure increased significantly (p less than 0.

[Investigations of the etiology of bradycardial rhythm disturbances (author's transl)].

As a possible etiology of cardiac conduction disturbances the sclerosis of coronary arteries and an idiopathic fibrosis are considered. In recent time also a diabetes-typical process in the coronary artery system has been discussed. Therefore we studied the distribution of atherosclerotic risk factors in 238 patients with severe cardiac conduction disturbances.

[Hemodynamic and electrophysiologic effects of the new calcium antagonist Ro 11-1781 (author's transl)].

In a 3-part study the new calcium-antagonist Ro 11-1781 was investigated in respect to hemodynamic, electrophysiologic and antiarrhythmic effects. In the first part of the investigation, systolic time intervals were determined as an indicator of left ventricular performance in 10 patients without cardiac disease before and 5 min after i.v.

Species similarities in pharmacokinetics.

There are many well documented similarities in the anatomy and physiology of mammalian species. There are also numerous examples in which the equilibrium distribution of foreign chemicals in the body appears to follow principles of thermodynamic partitioning with relatively minor interspecies variations to be expected. Information on metabolic pathways and their kinetic characteristics can be obtained from a variety of in vitro systems.