Publications by authors named "Birgit Stache-Crain"
Human papillomavirus (HPV) causes 5% of all cancers and frequently integrates into host chromosomes. The HPV oncoproteins E6 and E7 are necessary but insufficient for cancer formation, indicating that additional secondary genetic events are required. Here, we investigate potential oncogenic impacts of virus integration.
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- Human papillomavirus (HPV) is a key factor in some oral squamous cell carcinomas (OSCCs), with a notable rise in these cancers, but additional genetic changes are also involved.
- Comparative genomic analysis of HPV-positive and HPV-negative OSCCs revealed distinct mutational signatures reflecting different risk factors, with HPV-positive OSCCs showing a link to APOBEC editing and HPV-negative OSCCs linked to tobacco exposure.
- Specific genetic alterations and mutations were identified in HPV-positive OSCCs that affect crucial cellular pathways, suggesting that the interaction between the virus and the host's genetic makeup plays a significant role in the development of these cancers.
Copy number variation (CNV) is a common chromosomal alteration that can occur during in vitro cultivation of human cells and can be accompanied by the accumulation of mutations in coding region sequences. We describe here a systematic application of current molecular technologies to provide a detailed understanding of genomic and sequence profiles of human embryonic stem cell (hESC) lines that were derived under GMP-compliant conditions. We first examined the overall chromosomal integrity using cytogenetic techniques to determine chromosome count, and to detect the presence of cytogenetically aberrant cells in the culture (mosaicism).
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