Optimization of Triterpene Yield from Ganoderma atrum HBSD Z19 (Agaricomycetes) by Optimization of Medium Components and Evaluation of Bioactivity Under Simulated Gastrointestinal Digestion In Vitro.

The intracellular triterpene yield from Ganoderma atrum was enhanced by optimization based on single-factor experiments, Plackett-Burman experimental design (PBED) and response surface methodology (RSM) under liquid fermentation conditions. The optimal medium composition (g·L-1) was glucose (46.0), bean cake powder (30.

Transcriptome analysis revealing molecular mechanisms of enhanced pigment yield by succinic acid and fluconazole.

This study aimed to elucidate the molecular mechanisms through which succinic acid and fluconazole stimulate pigment biosynthesis under liquid fermentation conditions. The pigment yield was significantly improved by adding 0.35 g·L succinic acid or 1.