Publications by authors named "Binu K Mani"
Porcine reproductive and respiratory syndrome (PRRS) caused by an arterivirus is characterised by reproductive disorders in sows, and post-weaning pneumonia and growth reduction in piglets. Though the virus has been detected in Kerala, no systematic study has been carried out to ascertain its genotype and molecular epidemiology. In the present study, 7 PRRS virus (PRRSV) positive samples collected from incidences of PRRS in Kerala during 2017-2019 were subjected to ORF5, ORF7 and Nsp2 gene based reverse transcription polymerase chain reaction and the specific amplicons generated were sequenced.
View Article and Find Full Text PDFGroup A rotaviruses (GAR) are an important cause of diarrhoea in infants and newborn animals especially pigs. In this paper, we report the detection, G and P typing and phylogenetic analysis of GAR of pigs in Kerala. A total of 100 fecal samples from diarrhoeic piglets were collected from organized farms in Wayanad, Ernakulam, Thrissur, and Palakkad districts of Kerala.
View Article and Find Full Text PDFArticle Synopsis
- * Phylogenetic analysis showed that newly evolved isolates were closely related to South Indian strains, with specific mutations found in the isolates, including previously unreported mutations in one isolate.
- * The study also noted that some isolates had high similarity to vaccine strains but still caused outbreaks, marking the first comprehensive genetic characterization of IBDV in the region.
Aim: This study aims at cloning, sequencing, and phylogenetic analysis of a partial CDS of gene in pET-32a - DH5α system, with the objective of identifying the conserved nature of the gene in the genus .
Materials And Methods: A partial CDS (nucleotide 1873 to nucleotide 3363) of the gene was amplified from genomic DNA of serovar Canicola by polymerase chain reaction (PCR). The PCR-amplified DNA was cloned into pET-32a vector and transformed into competent DH5α bacterial cells.