and Evaluation of the Cytotoxic Potential of Hinokitiol against Osteosarcoma by Targeting Glycogen Synthase Kinase 3β.

Objectives: The present study aimed to assess the antiproliferative and pro-apoptotic effects of hinokitiol in osteosarcoma cells and targeting of glycogen synthase kinase 3 (GSK3).

Materials And Methods: The (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to evaluate the cytotoxic potential of hinokitiol in osteosarcoma cells. Various concentrations of hinokitiol (5, 10, 20, 40, 60, and 80 μg/mL) were tested, and the half-maximal inhibitory concentration (IC) was calculated.

Effect of hinokitiol in ameliorating oral cancer: in vitro and in silico evidences.

The study aimed to evaluate the anticancer potential of hinokitiol in treating oral cancer by using in vitro models and examining its interaction with the Pim-1 protein through in silico methods. Hinokitiol was applied to KB-1 oral squamous carcinoma cells, where the half-maximal inhibitory concentrations (IC) was determined. Morphologic changes in treated cells were observed using phase contrast microscopy, while acridine orange/ethidium bromide (AO/EB) staining was used to assess nuclear changes and apoptosis.

Exploring the potential therapeutic benefits of 7-methoxy coumarin for neuropathy pain: an in vivo, in vitro, and in silico approach.

Back Ground: 7-Methoxycoumarin (7-MC) is well recognized for its anti-inflammatory and anti-nociceptive actions. Its capacity to lessen neuropathic pain hasn't been documented yet. Hence the impact of 7-MC on vincristine-induced peripheral neuropathic pain in rodents was investigated.

"Therapeutic advancements in nanomedicine: The multifaceted roles of silver nanoparticles".

Nanotechnology has the advantages of enhanced bioactivity, reduced toxicity, target specificity, and sustained release and NPs can penetrate cell membranes. The small size of silver nanoparticles, AgNPs, large surface area, and unique physicochemical properties contribute to cell lysis and increased permeability of cell membranes used in the field of biomedicine. Functional precursors integrate with phytochemicals to create distinctive therapeutic properties and the stability of the nanoparticles can be enhanced by Surface coatings and encapsulation methods, The current study explores the various synthesis methods and characterization techniques of silver nanoparticles (AgNPs) and highlights their intrinsic activity in therapeutic applications, Anti-cancer activity noted at a concentration range of 5-50 μg/ml and angiogenesis is mitigated at a dosage range of 10-50 μg/ml, Diabetes is controlled within the same concentration.

Advances in nephroprotection: the therapeutic role of selenium, silver, and gold nanoparticles in renal health.

Renal toxicity is a disorder that causes considerable issues in healthcare systems world, highlighting the critical importance of creating alternative treatments. Metallic nanoparticles have recently emerged as promising therapeutic agents for nephroprotection because of their remarkable properties. Numerous disciplines, including medicine, biotechnology, and the food industry, are currently investigating and exploring metallic nanoparticles, such as selenium, silver, and gold, with promising outcomes.

Quantitative Determination of Related Substances in Formoterol Fumarate and Tiotropium in Tiomate Transcaps Dry Powder Inhaler.

Objectives: Tiotropium (TIO) and formoterol fumarate (FF) combination in dry powder inhaler (DPI) dosage form used for treating asthma, bronchospasm, chronic bronchitis, emphysema and chronic obstructive pulmonary diseases. Aim to develop an analytical method for estimating emerging and advancing dry powder inhaler combination toward enhanced therapeutics for the estimation of related substances but for this it is foremost to have a sensitive, simple, robust and validated method therefore, a new reverse phase-high performance liquid chromatography (HPLC) method has been developed for the determination of related substances in FF and TIO DPI.

Materials And Methods: The analytical method was performed on schimadzu HPLC with a quaternary pump, the separation achieved using BDS Hypersil C18 (250×4.

Anxiolytic-like activity of 5-methoxyflavone in mice with involvement of GABAergic and serotonergic systems - in vivo and in silico evidences.

Anxiety disorders are common worldwide and novel compounds are investigated for anxiolytic effect. A few studies have demonstrated the anxiolytic-like activity of natural and synthetic flavonoids. 5-methoxyflavone, a synthetic flavone derivative, has been reported to exhibit central nervous system depressant (sedative-hypnotic) effect in an earlier study.

Antinociceptive effect of flavonol and a few structurally related dimethoxy flavonols in mice.

Previous reports suggest flavonoids as potent analgesic compounds. Based on these observations, the present study investigated the antinociceptive action of flavonol, 3', 4'-dimethoxy flavonol, 6, 3'-dimethoxy flavonol, 7, 2'-dimethoxy flavonol, and 7, 3'-dimethoxy flavonol and the possible mechanisms involved in these effects. The antinociceptive effect of the investigated compounds in doses of 25, 50, 100, and 200 mg/kg was evaluated in male Swiss albino mice using the acetic acid test, formalin-induced nociception, and hot water tail immersion test.

Sedative-hypnotic like effect of 5-methoxyflavone in mice and investigation on possible mechanisms by in vivo and in silico methods.

Flavonoids have been shown to possess central nervous system (CNS) depressant effect mediated through the ionotropic GABA receptors. In the present study, 5-methoxyflavone was evaluated for sedative-hypnotic like activity in mice and the mechanisms involved by employing a battery of tests including molecular docking studies. In the open field test, 5-methoxyflavone in various doses (50, 100 and 150 mg/kg, i.

Effect of certain trimethoxy flavones on paclitaxel - induced peripheral neuropathy in mice.

Background: The anti - nociceptive effect of 7, 2', 3' - trimethoxy flavone, 7, 2', 4' - trimethoxy flavone, 7, 3', 4' - trimethoxy flavone and 7, 5, 4' - trimethoxy flavone against inflammatory, neurogenic and thermal pain in mice was reported earlier. The present study was designed to investigate the effect of the above trimethoxy flavones in amelioration of peripheral neuropathy induced by paclitaxel.

Methods: Peripheral neuropathy was induced in mice by administration of a single i.

Anti-nociceptive Effect of 7-methoxy Coumarin from (Asteraceae).

Aim: To evaluate the anti-nociceptive activity of 7-methoxy coumarin isolated from ethyl acetate fraction of the alcoholic extract of .

Materials And Methods: The shade dried leaves of were extracted with ethyl alcohol and the extract was condensed. This extract was fractionated with n-hexane, ethyl acetate, and n-butanol.

Anti-nociceptive activity of a few structurally related trimethoxy flavones and possible mechanisms involved.

Background: The present study was designed to investigate the anti-nociceptive activity of a few structurally related trimethoxy flavones (7,2',3'-TMF, 7,2',4'-TMF, 7,3',4'-TMF and 7,5,4'-TMF) and the possible mechanisms involved.

Methods: Anti-nociceptive activity was evaluated in mice by employing acetic acid-induced writhing, formalin-induced nociception and hot water tail immersion methods. The involvement of opioid, GABAergic, tryptaminergic, adrenergic and dopaminergic mechanisms and K+ATP channels in the anti-nociceptive activity of trimethoxy flavones was investigated using suitable interacting chemicals.