ISG15 is associated with cervical cancer development.

Cervical cancer (CC) is a complex disease. Numerous factors contribute to the tumourigenesis and progression of CC neoplasms. The present study analysed transcriptomic differences and simulated tumour progression to explore the pathogenesis of CC.

The overexpression of lncRNA MEG3 inhibits cell viability and invasion and promotes apoptosis in ovarian cancer by sponging miR-205-5p.

Purpose: Ovarian cancer is a common and fatal cancer in women. The long non-coding RNA (lncRNA) MEG3 was reported to affect the cellular processes of ovarian cancer, but the mechanisms remain unclear. Here, we aimed to explore the potential regulatory mechanism of MEG3 in ovarian cancer.

Evaluation of p16/Ki-67 Dual-Stained Cytology in Triaging HPV-Positive Women during Cervical Cancer Screening.

Background: We aimed to evaluate the utility of p16/Ki-67 dual-stained cytology for triaging human papillomavirus (HPV)-positive women.

Methods: HPV-positive women ages ≥ 21 years were recruited in a multicenter prospective observational study between May 2016 and May 2017. The clinical performance of dual-stained cytology, with or without HPV16/18 genotyping, was evaluated for all HPV-positive women to detect cervical intraepithelial neoplasia grade 2 or worse (CIN2+).

Variant sublineages of human papillomavirus type 16 predispose women to persistent infection characterized by a sequence analysis of the E6, L1, and LCR regions.

Background: One of the precursors of cervical cancer is persistent infection with human papillomavirus (HPV), especially high-risk HPV. The aim of this study was to verify the relationship between HPV16 variants and persistent viral infection.

Methods: Three-hundred and eighty-six Chinese women who had a low-grade squamous intraepithelial lesion (LSIL) or a lesion below LSIL with normal cellular morphology were selected and enrolled in this study.

miR-144 inhibits growth and metastasis of cervical cancer cells by targeting VEGFA and VEGFC.

MicroRNAs (miRs) are aberrantly expressed in various cancer types and have critical roles in their genesis and progression. miR-144 has been identified to be involved in the development of hepatocellular carcinoma and rectal cancer. However, the roles of miR-144 in cervical cancer and the underlying molecular mechanisms have remained elusive.

Effect of paiteling on human papillomavirus infection of the cervix.

The aim of the present study was to investigate the effect of the traditional Chinese medicine paiteling on the outcome of high-risk human papillomavirus (HR-HPV) infection of the cervix. A total of 321 patients were enrolled in this study and HPV subtypes were determined by the Hybribio HPV genotyping system. The patients were divided into the loop electrosurgical excision procedure (LEEP; n=82) and non-LEEP (n=239) groups, according to the cervical intraepithelial neoplasia classification.

Aryl hydrocarbon receptor and unexplained miscarriage.

Aim: The aim of this study was to carry out a preliminary investigation of the expression of aryl hydrocarbon receptor (AhR) in decidua and villus and the relationship between AhR and unexplained miscarriage.

Methods: The expression of AhR mRNA and protein from decidua and villus were measured using real-time reverse transcription-polymerase chain reaction, western blot and immunohistochemistry in 34 patients with unexplained miscarriage (miscarriage group) and 38 women with normal early pregnancy (control group).

Results: The AhR mRNA and protein expression was increased significantly in the villus in both groups compared with decidua (P < 0.

Association between human papillomavirus type 16 E6 and E7 variants with subsequent persistent infection and recurrence of cervical high-grade squamous intraepithelial lesion after conization.

The study aimed to detect the variants of human papillomavirus (HPV) type 16 E6 and E7 in patients with cervical high-grade squamous intraepithelial lesion (HSIL), and to determine the existence and recurrence of persistent infection after treatment with loop electrosurgical excision procedure (LEEP). Preoperatively collected cervical exfoliated cells from 100 HPV 16 positive HSIL patients enrolled in the study were used to test for E6 and E7 variants. Follow-ups which included TCT, HPV test, and colposcopy were performed every 3 months after the operation, and colposcopic biopsy and endocervical curettage were performed for patients with abnormalities.

Variants of human papillomavirus type 16 predispose toward persistent infection.

A cohort study of 292 Chinese women was conducted to determine the relationship between human papillomavirus (HPV) type 16 variants and persistent viral infection. Enrolled patients were HPV16 positive and had both normal cytology and histology. Flow-through hybridization and gene chip technology was used to identify the HPV type.

Ovarian serous carcinogenesis from tubal secretory cells.

Due to a poor understanding of tumorigenesis, ovarian cancers remain the most lethal gynecologic malignancy and cause horrific deaths. In the last decade, a new dualistic model for ovarian cancer was proposed, wherein ovarian serous cancers are classified as either high-grade or low-grade, with each having different tumorigenic processes, and pathologic and clinical features. Surprisingly, both high- and low-grade ovarian serous cancers were recently found to originate not in the ovaries, but rather from the secretory cells of the fallopian tube, mostly from the tubal fimbriated ends.

Doxycycline Induces Apoptosis and Inhibits Proliferation and Invasion of Human Cervical Carcinoma Stem Cells.

Background: Cancer stem cells (CSCs) are proposed to be responsible for high recurrence rate in cervical carcinoma. Reagents that can suppress the proliferation and differentiation of CSCs would provide new opportunities to fight against tumor recurrence. Doxycycline has been reported as a potential anti-cancer compound.

Aberrant expression of E-cadherin and integrin β-1 in trophoblasts is associated with malignant gestational trophoblastic diseases.

Objective: To investigate the association between malignant gestational trophoblastic diseases and aberrant local expression of E-cadherin and integrin β-1 in the context of identifying a method to predict and diagnose malignant gestational trophoblastic diseases at an early stage.

Methods: We used immunohistochemistry to assess E-cadherin and integrin β-1 expression profiles in normal chorionic villus, hydatidiform mole, and invasive mole.

Results: E-cadherin expression gradually decreased from normal tissue to malignant tissue.

Efficacy of Pap test in combination with ThinPrep cytological test in screening for cervical cancer.

Background: Our aim was to investigate the efficacy of the Pap test in combination with the ThinPrep cytological test (TCT) in screening for cervical cancer in China.

Design: From March 2006 to October 2008, 988 women with the mean age 46.4 ± 10.

[Survey on menopausal age and menstruation span in women in Pudong district of Shanghai].

Objective: To investigate natural spontaneous menopausal age, menstruation span and their relationship with menarche age and parity in Pudong district of Shanghai.

Methods: From Jan 2007 to Jul 2008, 15 083 spontaneous menopause women undergoing cervical cancer screening were enrolled in this study. The questionnaire included menarche age, parity, spontaneous menopausal age and menstruation span.

[Diagnosis of stress urinary incontinence using poster urethrovesical angel measured by ultrasonography].

Objective: To evaluate the clinical value of ultrasonography detecting the poster urethrovesical angle (PUVA) in diagnosis of stress urinary incontinence (SUI).

Methods: From Jan. 2006 to Dec.

Personal history of breast cancer as a significant risk factor for endometrial serous carcinoma in women aged 55 years old or younger.

A comparative study between endometrial serous carcinoma (ESC) and endometrial endometrioid carcinoma (EEC) was performed to determine whether a personal history of breast cancer is a risk factor for ESC in women aged ≤ 55 yr. Study subjects consisted of 348 women who were diagnosed with ESC and 830 comparison subjects who had EEC. Variables studied included age at diagnosis, a history of breast cancer, tamoxifen therapy, hormonal replacement therapy and smoking history.

Therapy with FasL-gene-modified dendritic cells confers a protective microenvironment in murine pregnancy.

Analysis of the expression of FasL in the local decidua of pregnant mice and examination of the apoptosis of T cells in peripheral blood and local decidua indicated that adoptive transference of FasL-gene-modified dendritic cells may induce pregnancy immune tolerance by increasing FasL expression in the maternal-fetal interface and inducing the apoptosis of T cells in local decidua but not the peripheral blood.

[Survey on age of menarche in 56,924 women recruited from Pudong district of Shanghai].

Objective: To survey age of menarche in Pudong district in Shanghai.

Methods: Data in this study were derived from 56,924 women at age of 20-81 years in screening for cervical cancer between January 2007 and July 2008 in Pudong district. The age of menarche were recorded in a questionnaire.

[Involvement of matrix metalloproteinase-2, -9, and tissue inhibitors of metalloproteinase-1, 2 in occurrence of the accrete placenta].

Objective: To investigate the roles of matrix metalloproteinase-9, -2 (MMP-9, 2), and tissue inhibitors of metalloproteinase-1, 2 (TIMP-1, 2) in pathogenesis of the accretio placenta.

Methods: The women with the placenta accrete were recruited and the placenta (23) and deciduas tissues (9) after labor were obtained, and the placenta (28) and deciduas (11) from women without the placenta accreta were obtained as control to get, too. The expressions of MMP-9, -2, TIMP-1, 2 in the placental and decidual tissues were analyzed by real-time PCR.