Short-term efficacy of open surgery versus arthroscopic rotator cuff repair in moderate rotator cuff tears.

Objective: To evaluate the clinical efficacy of open versus arthroscopic rotator cuff repair in treating moderate rotator cuff tears.

Methods: A retrospective study was conducted on 68 patients diagnosed with moderate rotator cuff tears and who were treated surgically at Hangzhou Fuyang Hospital of Traditional Chinese Medicine Orthopedics and Traumatology from September 2021 to April 2023. Patients were categorized based on the surgical methods they received: the minimally invasive group (MIG, n=37, undergoing arthroscopic rotator cuff repair) and the open surgery group (OSG, n=31, undergoing open rotator cuff repair).

A closed-tube detection of loop-mediated isothermal amplification (LAMP) products using a wax-sealed fluorescent intercalator.

LAMP is an isothermal amplification method that can achieve ultra-high sensitivity and specificity. However, the conventional detection of LAMP amplicons can lead to cross-contamination due to the need to open the reaction tube which contains a large number of amplicons. To achieve closed-tube LAMP detection, we have developed a method that separates a solution of SYBR Green I (SGI) from the LAMP reagents using temperature-sensitive wax.

Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.

Somatic mutations in stool DNA are quite specific to colorectal cancer (CRC), but a method being able to detect the extraordinarily low amounts of mutants is challengeable in sensitivity. We proposed a hydrogel bead-array to digitally count CRC-specific mutants in stool at a low cost. At first, multiplex amplification of targets containing multiple mutation loci of interest is carried out by a target enriched multiplex PCR (Tem-PCR), yielding the templates qualified for emulsion PCR (emPCR).