Publications by authors named "Binh Thanh Vo"
For over a decade, the animal field has led the way in using DNA methylation measurements to construct epigenetic clocks of aging. These clocks can predict organismal age with a level of accuracy that surpasses any other molecular proxy known to date. Evidence is finally emerging that epigenetic clocks also exist in plants.
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- Targeted therapy using tyrosine kinase inhibitors (TKI) can improve survival rates for many patients with non-small cell lung cancer (NSCLC), but resistance to these treatments often develops.
- A study analyzed the genetic and epigenetic changes in 122 Vietnamese NSCLC patients experiencing resistance to TKI therapy, finding that 41.8% had specific resistance mutations, particularly in the EGFR gene.
- The research highlighted that the level of genome-wide hypomethylation was linked to how long patients responded to TKI, suggesting that liquid biopsies can help understand TKI resistance mechanisms and inform future treatment strategies.
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- Population-specific profiling of cancer gene mutations is essential for better understanding cancer biology and improving diagnostics and treatment tailored to specific groups.
- The study used ultra-deep massive parallel sequencing of plasma cell-free DNA (cfDNA) to analyze mutations in 265 Vietnamese patients with advanced non-small cell lung cancer, offering a less invasive alternative to traditional tumor tissue analysis.
- Although cfDNA testing had lower mutation detection rates, it still identified major mutations in key driver genes that were consistent with findings from tissue sample analysis, highlighting its potential for large-scale genetic profiling in populations with limited access to tumor biopsies.
Article Synopsis
- - Comprehensive profiling of mutations in non-small cell lung cancer (NSCLC) is crucial for guiding targeted therapies and improving patient survival, especially in high-incidence regions like Vietnam.
- - A study involving 350 Vietnamese NSCLC patients identified that mutations in the EGFR gene (35.4%) and KRAS gene (22.6%) were the most common, with notable differences compared to other ethnic cohorts.
- - The research found that KRAS mutations were more prevalent in males, while EGFR mutations were more frequent in females, and younger patients (<61 years) showed higher rates of ALK and ROS1 rearrangements.
The identification and quantification of actionable mutations are critical for guiding targeted therapy and monitoring drug response in colorectal cancer. Liquid biopsy (LB) based on plasma cell-free DNA analysis has emerged as a noninvasive approach with many clinical advantages over conventional tissue sampling. Here, we developed a LB protocol using ultra-deep massive parallel sequencing and validated its clinical performance for detection and quantification of actionable mutations in three major driver genes ( and ).
View Article and Find Full Text PDFThe identification and quantification of actionable mutations are of critical importance for effective genotype-directed therapies, prognosis and drug response monitoring in patients with non-small-cell lung cancer (NSCLC). Although tumor tissue biopsy remains the gold standard for diagnosis of NSCLC, the analysis of circulating tumor DNA (ctDNA) in plasma, known as liquid biopsy, has recently emerged as an alternative and noninvasive approach for exploring tumor genetic constitution. In this study, we developed a protocol for liquid biopsy using ultra-deep massively parallel sequencing (MPS) with unique molecular identifier tagging and evaluated its performance for the identification and quantification of tumor-derived mutations from plasma of patients with advanced NSCLC.
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