Tyrosinase Oxidative Cross-Linking in the Cell-Like Crowded Microenvironment for Visible Inhibitor Screening.

Owing to many diseases and disorders being caused by dysfunctional or over/underexpressed enzymes, screening for inhibitors of pharmacologically relevant enzymes has emerged as a promising tool for drug discovery, clinical diagnostics, enzyme engineering, and other medical fields. However, despite the recent advances, most inhibitor screenings are still usually conducted in dilute media, at concentrations far from the media in which the enzymes are actually found, which may cause drugs to fail when translated to . Herein, we build a gel-like intracellular biological environment using a tyrosinase oxidative cross-linking hydrogel system that is closer to the real catalytic environment of enzymes.