Publications by authors named "Binglin Tan"
Article Synopsis
- Researchers identified a pathogenicity island (PAI) of about 32-kb in Yersinia entomophaga MH96, which contains key genes responsible for insecticidal properties, including toxin complex components and chitinases.
- Deleting the central region of the PAI (which includes the toxin genes) rendered the bacteria harmless to Costelytra zealandica larvae, demonstrating the importance of these genes for virulence.
- When the researchers reintroduced the complete TC gene region into a mutant strain, it restored its ability to kill insects, highlighting the significant role of the toxin complex in the bacterium's pathogenicity.
Article Synopsis
- - Serratia entomophila and Serratia proteamaculans cause a disease called amber disease in grass grub larvae (Costelytra zealandica) due to three virulence genes, sepABC, found on a large plasmid (pADAP).
- - The sep genes encode proteins that are part of a toxin complex that targets insects, and researchers used an inducible promoter to control the expression of these genes for experiments.
- - Bioassays revealed that symptoms of amber disease in larvae only occurred when all three sep genes were expressed together, and after two weeks, about 64% of infected larvae showed recovery, although re-exposure to the Sep protein caused the disease to return.
Strains of Serratia spp. showed a high level of virulence when injected into the hemocoel of larvae Costelytra zealandica, with Serratia entomophila, S. plymuthica, and S.
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