Preparation and evaluation of ultra-long open-tubular capillary columns modified with zeolitic imidazolate framework-8 incorporated polymeric porous layer for liquid chromatography.

An ultra-long (5 m) open tubular capillary liquid chromatographic column was prepared by incorporating Metal Organic Framework (MOF), zeolitic imidazolate framework-8 (ZIF-8), directly into polymer coating, which was synthesized by the copolymerization of 4-vinylbenzyl chloride and divinylbenzene, on the capillary inner surface. The prepared ZIF-8 incorporate polymeric open tubular capillary column (denoted as ZIF-8-p(VBC/DVB) OTCC) was evaluated with thiourea, alkylbenzenes and polycyclic aromatic hydrocarbons as probe molecules. The results showed that the ultra-long column achieved absolute column efficiency of 130,000 plates for thiourea, and the incorporation of ZIF-8 effectively improved the chromatography performance of the OTCC.

[Preparation and evaluation of a new type of amino-bonded silica chromatographic stationary phase for the determination of lactose].

A method for the separation and determination of lactose and sucrose using high performance liquid chromatography-refractive index (HPLC-RI) detection base on the prepared amino-bonded silica gel chromatographic stationary phase was established. The method was accorded to the content determination method in Pharmacopoeia of the People's Republic of China (2015). Then, a series of chromatographic behaviors of lactose and sucrose such as the retention times, resolutions and peak area stabilities were investigated on three different types of amino-bonded silica columns (300 mm×4.