Variations in gut microbiota composition and reproductive hormone levels between laying and broody Muscovy ducks.

High broodiness in Muscovy ducks impedes animal husbandry growth. The interaction between endocrine hormones and gut microbiota has been proven to play a crucial role in reproductive performance, and whether it can regulate the broody behavior of Muscovy ducks requires further research. Nine laying ducks (Laying group) and nine broody ducks (Broodiness group) were selected.

A DNA machine-based magnetic resonance imaging nanoprobe for in vivo microRNA detection.

In situ monitoring microRNA (miRNA) expression in vivo holds immense potential for directly visualizing the occurrence and progression of tumors. However, the significant barrier to developing a probe that can overcome the low abundance of miRNAs while providing an output signal with unlimited tissue penetration depth remains formidable. In this study, we developed a DNA machine-based magnetic resonance imaging nanoprobe (MRINP) for amplified detection of miR-21 in vivo.

Advancements in the synthesis of carbon dots and their application in biomedicine.

As a sort of fluorescent carbon nanomaterial with a particle size of less than 10 nm, carbon dots (CDs) have their own merits of good dispersibility in water, stable optical properties, strong chemical inertness, stable optical properties, and good biosecurity. These excellent peculiarities facilitated them like sensing, imaging, medicine, catalysis, and optoelectronics, making them a new star in the field of nanotechnology. In particular, the development of CDs in the fields of chemical probes, imaging, cancer therapy, antibacterial and drug delivery has become a hot topic in current research.

Preparation, Structural Characterization, and Enzymatic Properties of Alginate Lyase Immobilized on Magnetic Chitosan Microspheres.

Alginate lyase is an enzyme that catalyses the hydrolysis of alginate into alginate oligoalginates. To enhance enzyme stability and recovery, a facile strategy for alginate lyase immobilization was developed. Novel magnetic chitosan microspheres were synthesized and used as carriers to immobilize alginate lyase.

Visible-light-catalyzed synthesis of 1,3-benzoxazines formal [4 + 2] cycloaddition of oximes with -hydroxybenzyl alcohols.

A formal [4 + 2] cycloaddition of oximes with -hydroxybenzyl alcohols was developed to easily synthesize diverse 1,3-benzoxazine derivatives. This synthesis was achieved under visible light-based organocatalytic and TsOH conditions. The reaction proceeds through the photoisomerization of oximes visible light-mediated energy transfer, followed by the nucleophilic attack of -QMs to oximes as a 1,2-dipole synthon, cyclization, and isomerization.

Evaluation of non-invasive gene detection in preimplantation embryos: a systematic review and meta-analysis.

Background: Genetic abnormalities in embryos are responsible for most miscarriages and repeated embryo implantation failures, so a reliable preimplantation genetic screening method is urgently needed. Non-invasive preimplantation genetic testing (niPGT) is a potential method for embryo genetic diagnosis. However, the value of its application is controversial.

Multidimensional Evaluation of the Quality of Rural Life Using Big Data from the Perspective of Common Prosperity.

Evaluating and revealing the spatial differentiations of quality of rural life (QRL) is the basis for formulating rural revitalization planning to promote rural transformation and achieve common prosperity. Taking the Lin'an District of Hangzhou city in China, an economically developed mountainous area, as an example, this study explored the connotation of QRL from the perspective of common prosperity and constructed a QRL evaluation framework involving living, employment, consumption, and leisure aspects. Then, based on multi-sourced data of 270 administrative villages as the assessment unit, we revealed the spatial patterns of QRL and proposed optimization paths to improving QRL.

Enantioselective Total Synthesis of (+)-Sieboldine A and Analogues Thereof.

An 11-step enantioselective total synthesis of (+)-sieboldine A () has been accomplished from (5)-methylcyclohex-2-en-1-one (), in which an intramolecular ketone/ester reductive coupling followed by one-pot acidic treatment to quickly construct the unique oxa-spiroacetal and a TsOH-catalyzed displacement to directly form the characteristic -hydroxyazacyclononane ring successfully served as the key methodologies. Moreover, several full-skeleton analogues of were also synthesized on the basis of the advanced intermediates, and their inhibitory effects on electric eel acetylcholinesterase were examined.

A Study on the Impact of Low-Carbon Technology Application in Agriculture on the Returns of Large-Scale Farmers.

The relationship and mechanism between agricultural low-carbon technology application and farm household returns are not yet clear, especially the lack of evidence from developing countries. This paper takes large-scale farming households in Jiangxi Province, China, from 2019 to 2020 as the research object, and obtains relevant data from field research to explore the intrinsic impact of agricultural low-carbon technology application on the returns of large-scale farming households. Based on the relevant theoretical analysis, the division dimensions of agricultural low-carbon technologies were proposed, and agricultural low-carbon technologies were subdivided into ten specific low-carbon technologies according to six dimensions: tillage system, breeding, fertilization, irrigation, medicine application, and waste treatment.

Unified Total Synthesis of Tetracyclic Diquinane Alkaloids (+)-Paniculatine, (-)-Magellanine, and (+)-Magellaninone.

A unified route for the total synthesis of three tetracyclic diquinane alkaloids (+)-paniculatine, (-)-magellanine, and (+)-magellaninone has been accomplished in 13-14 overall steps based on late-stage diverse transformations from an advanced tetracyclic common intermediate. In the established synthesis, quick formation of the two five-membered rings was efficiently achieved by an intramolecular reductive coupling of ketone-carbonyl and ester-carbonyl and an organocatalytic intramolecular Michael addition of aldehyde-derived enamine to an internal enone functionality with satisfactory redox and step economies and excellent stereoselectivities, providing the requisite tricyclic carbo-framework possessing multiple dense stereogenic centers, and an intramolecular reductive amination finally furnished the essential piperidine ring.

The Potential of Gut Microbiota Metabolic Capability to Detect Drug Response in Rheumatoid Arthritis Patients.

Gut microbiota plays an essential role in the development of rheumatoid arthritis (RA) and affects drug responses. However, the underlying mechanism remains elusive and urgent to elucidate to explore the pathology and clinical treatment of RA. Therefore, we selected methotrexate (MTX) as an example of RA drugs to explore the interactions between the gut microbiota and drug responses and obtain an in-depth understanding of their correlation from the perspective of the metabolic capability of gut microbiota on drug metabolism.

lncRNA DSCAM-AS1 facilitates the progression of endometrial cancer via miR-136-5p.

Previous studies have indicated that long non-coding RNA (lncRNA) down syndrome cell adhesion molecule antisense 1 (DSCAM-AS1) serves an oncogenic role in numerous cancer types. However, its role in endometrial cancer (EC) remains largely unknown. In the present study, DSCAM-AS1 expression levels in EC tissues and cells and their normal counterparts were analyzed using reverse transcription-quantitative.

Effects of Defatted Rice Bran Inclusion Level on Nutrient Digestibility and Growth Performance of Different Body Weight Pigs.

This study was conducted to determine the effects of low-protein diet prepared with different levels of defatted rice bran (DFRB) and weight stages on growth performance and nutrient digestibility of growing-finishing pigs. The animal experiment included three stages. A total of 240 growing pigs with an initial body weight of 28.

Evaluation on Net Energy of Defatted Rice Bran from Different Origins and Processing Technologies Fed to Growing Pigs.

The study was conducted to determine and compare the net energy (NE) of defatted rice bran (DFRB) from different sources and different processing technology fed to growing pigs using indirect calorimetry. Thirty-six growing barrows (30.7 ± 3.

Concise Unified Access to (-)-8-Deoxy-13-dehydroserratinine, (+)-Fawcettimine, (+)-Fawcettidine, and (-)-8-Deoxyserratinine Using a Direct Intramolecular Reductive Coupling.

A short, scalable, and collective total synthesis of four fawcettimine-type alkaloids in eight or nine steps is disclosed. A dense multi-small-ring -α-aminocyclopentanone successfully served as the key intermediate, which was directly accessed by a LiDBB-mediated intramolecular reductive coupling of the aliphatic imine and an ester-carbonyl. Compared to those that employ classical Heathcock intermediate(s) containing a nine-membered ring, the new strategy shows the significant improvement of the synthetic step and redox economies as well as excellent stereochemical control.

Effects of different defatted rice bran sources and processing technologies on nutrient digestibility in cannulated growing pigs.

The objective of this study was to evaluate the effects of different defatted rice bran (DFRB) sources and processing technologies on nutrient digestibility in different intestinal segments of pigs. Nine barrows with T-cannula in the distal ileum were randomly allotted to nine different sources in which oil was pressed extracted for seven sources and was solvent extracted for two sources. The experiment contained 6 periods of 12 d, including 8 d for diet adaptation, 2 d for fecal collection, and 2 d for digesta collection.

Comparison of regression and fat-free diet methods for estimating ileal and total tract endogenous losses and digestibility of fat and fatty acids in growing pigs.

An experiment was conducted to 1) compare the regression and fat-free diet methods for estimating total or basal endogenous losses of fat (ELF) and fatty acids (ELFA) and true digestibility (TD) or standardized digestibility (SD) of fat and fatty acids in growing pigs and 2) compare these estimated values at the end of the ileum and over the entire intestinal tract. Ten barrows (initial body weight: 45.1 ± 2.

Endogenous Losses of Fat and Fatty Acids in Growing Pigs Are Not Affected by Vegetable Oil Sources but by the Method of Estimation.

An experiment was conducted to determine the effect of oil sources with differing degrees of fatty acid saturation on endogenous losses of fat (ELF) and fatty acids (ELFA) in growing pigs, in which endogenous losses were estimated by two methods. Sixty-eight growing barrows (initial body weight 31.13 ± 4.

Net energy of oat bran, wheat bran, and palm kernel expellers fed to growing pigs using indirect calorimetry.

The objectives of this experiment were to (a) determine the effects of fiber increase in diets on heat production (HP), (b) determine the net energy (NE) of oat bran (OB), wheat bran (WB), and palm kernel expellers (PKE) fed to growing pigs using indirect calorimetry (IC). Twenty-four growing barrows (29.2 ± 2.

Available energy and amino acid digestibility of defatted rice bran fed to growing pigs.

This study was conducted to determine and compare available energy and apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of CP and AA in 9 samples of defatted rice bran (DFRB) fed to growing pigs and to generate prediction equations for DE and ME based on chemical analysis. In Exp. 1, 60 crossbred growing pigs (Duroc × Landrace × Yorkshire; 40.

Enantioselective Dearomative Difunctionalization of Indoles by Palladium-Catalyzed Heck/Sonogashira Sequence.

Palladium-catalyzed enantioselective dearomative arylalkynylation of N-substituted indoles, through a Heck/Sonogashira sequence, was established using a new BINOL-based phosphoramidite as the chiral ligand. A wide range of 2,3-disubstituted indolines, bearing vicinal quaternary and tertiary stereocenters, were efficiently constructed in one step with excellent enantioselectivities (up to 97 % ee) and diastereoselectivities (>20:1).

Comparison of enhancement of pentachlorophenol sonolysis at 20 kHz by dual-frequency sonication.

The comparison of enhancement effect of pentachlorophenol sonolysis at 20 kHz by different dual-frequency ultrasonic irradiations has been investigated. Dual-frequency (20 kHz/40 kHz, 20 kHz/530 kHz, 20 kHz/800 kHz and 20 kHz/1040 kHz) ultrasounds have been used. It has been found that the rate of pentachlorophenol degradation at dual-frequency ultrasonic irradiation is the highest compared to mono-frequency ultrasonic systems.