Publications by authors named "Bing-shen Ke"
[Sequence analysis of a novel human leukocyte antigen allele B*5827].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
February 2011
Objective: To investigate the molecular basis for a novel human leukocyte antigen (HLA) allele B*5827.
Methods: DNA from the proband was analyzed by polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO) typing. The amplified product was sequenced bidirectionally.
The PCR technique has been set up for nearly twenty years and is becoming more and more ripe. But because of the multiple influencing factors and complicated reaction procedures,no mathematical method that can describe the PCR reaction has been given. On the basis of its elementary principle,we suggested a kinetic equation to describe the reaction procedure,Wamp=[Ntarg x (1+P)n1+0.
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- Current standard PCR methods are inadequate for biotech and clinical research, prompting the need for a better quantitative PCR technique.
- PE company discovered a linear relationship between initial template number and cycling time, leading to the development of a quantitative PCR technique, though it still suffers from high error rates.
- A new mathematical model integrates scientific principles and accurately describes the relationship between product quantity, fluorescence intensity, and initial template number, promising improved accuracy and significantly lower error rates in quantitative PCR analysis.
[Study on mitochondrial DNA gene tRNA(Leu(UUR)) A3243G mutation in type 2 diabetes mellitus].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
April 2004
Objective: To explore the prevalence and the clinical characteristics of mitochondrial gene mutation A3243G (mt tRNA(Leu(UUR)) 3243 A-->G) in patients with type 2 diabetes mellitus (DM2) in China.
Methods: Four hundred and twenty-eight cases of DM2 patients were selected randomly. One hundred and eighty-eight individuals were healthy controls.