[The effect of danshen on the angiogenesis of the frozen-thawed human fetal ovarian tissue after transplantation].

Aim: (1) To investigate the mRNA expression of the key angiogenic growth factors in the grafts after transplantation. (2) To investigate the potential impact of danshen (Chinese traditional medicine) administration on grafts angiogenesis.

Methods: The frozen-thawed ovarian tissue from aborted fetus were xenografted into the renal capsule of the nude mice, recovered 48 h, 7 d and 28 d after respectively.

[A study on bisulfite sequencing method for methylation status of imprinted genes in single human oocytes].

Objective: To establish and improve the method of bisulfite sequencing for methylation status of imprinted genes in single human oocytes.

Methods: Single superovulated immature human oocyte was embedded into low melting point agarose, followed by bisulfite treatment and polymerase chain reaction (PCR) amplification of the H19 and MEST genes. The PCR products were then subjected to TA cloning and sequencing to determine the methylation status.

[The correlation between ICAM-1 gene K469E polymorphism and coronary heart disease].

Objective: To investigate the correlation between intercellular adhesion molecule1 (ICAM1) gene K469E polymorphism and coronary heart disease(CHD) in Han Chinese population.

Methods: Using the methods of polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP), 173 CHD patients and 141 controls were analyzed for the polymorphism, genotype and allele distribution of ICAM1 gene K469E.

Results: The distribution of ICAM1 genotypes was in Hardy-Weinberg equilibrium.

[HLA-A site genotyping on single blastomeres is studied by nest-PCR-SSP method].

Objective: To assess the accuracy and reliability of the nest-PCR-sequence specific primer(SSP) method in HLA-A site genotyping of single blastomeres retrieved from human pre-implantation embryos.

Methods: By nest PCR on HLA-A exon 2, the success rate of first-round amplification was estimated for single blastomeres. Based on the first-round amplification, the HLA-A genotype of every single blastomeres was analyzed by commercially available PCR-SSP kits.