[Clinical application of 320-row computed tomography 4D digital subtraction angiography in hepatocellular carcinoma].

Objective: To explore the application value of 320-row computed tomography (CT) 4D digital subtraction angiography (DSA) for hepatocellular carcinoma (HCC).

Methods: A total of 40 HCC patients received 320-row CT contrast scans. The 4D DSA images were obtained on the basis of baseline data.

[The mechanism of inhibition of the increase in intracellular calcium concentration by the islet amyloid polypeptide in high glucose-stimulated INS-1 cells].

Objective: To explore the potential mechanism of the inhibition of increased intracellular free calcium concentration ([Ca²⁺]i) by short-term exposure to the islet amyloid polypeptide (IAPP) in high glucose-stimulated pancreatic β cells.

Methods: The pancreatic β cells were loaded with calcium sensitive fluorescent indicator Fluo-4/AM. The fluorescence intensity, which represented [Ca²⁺]i, was measured in time by laser scanning confocal microscope before and after stimulated by glucose, KCl, caffeine and carbachol.

[To evaluate the role of OLT on splenomegaly of portal hypertension by the radiological changes of splenic morphology and collaterals].

Objective: To explore the effect of orthotopic liver transplantation (OLT) on portal hypertension by observing the radiological changes of splenic volume and collaterals before and after OLT.

Methods: In our hospital 56 patients performing OLT due to cirrhosis, portal hypertension and splenomegaly were classified into five groups according to their following-up time: A (≤3 months), B (>3-6 months), C (>6-12 months), D (>12-24 months), and E (>24 months). Twenty health people were chose as control group (F).

[Application value of diffusion-weighted imaging in ischemic-type biliary lesions after liver transplantation].

Objective: To discuss the application values of DWI (diffusion-weighted imaging) and ADC (apparent diffusion coefficient) on ischemic-type biliary lesions (ITBL) after orthotopic liver transplantation.

Methods: According to whether there was ITBL after liver transplantation or not, 46 cases of liver transplantation were selected and divided into 2 groups on the basis of PTC (percutaneous transhepatic cholangiography) or ERCP (endoscopic retrograde cholangiopancreatography) examination, pathology or clinical follow-up data: ITBL group (n = 29) and no ITBL group (C group, n = 17). The ADC value was measured for right lobe of graft liver parenchyma (b value = 600 s/mm(2)).

[Diagnostic value of multislice spiral CT and MRI in detection of tumor recurrence after liver transplantation for hepatocellular carcinoma].

Objective: To investigate the manifestation and diagnostic value of multislice spiral CT (MSCT) and MRI imaging in detection of tumor recurrence after liver transplantation for hepatocellular carcinoma (HCC).

Methods: The clinical data of 161 consecutive HCC patients who underwent orthotopic liver transplantation were retrospectively reviewed. Twenty-nine HCC patients were classified by pTNM according to the "Pittsburgh criteria".

[The effects of amylin on the islet beta-cells voltage-gated L-calcium channels in rats].

Objective: To observe the effect of amylin on the islet beta-cells voltage-gated L-calcium channels in rats.

Method: Patch clamp technique was employed in the observation of the features and changes of electric current of islet beta-cells voltage-gated L-calcium channels before and after using amylin.

Results: In the glucose environment of 5.

[Inhibitory effect of unaggregated amyloid beta protein (25-35) on delayed rectifier potassium current in rat hippocampal CA3 pyramidal neurons].

Objective: To investigate the effect of unaggregated Abeta(25.35) on delayed rectifier potassium current (I(K)) in neonatal rat hippocampal CA3 pyramidal neurons.

Methods: The rat hippocampal neurons were enzymatically isolated from 10-11-day-old Wistar rat.

Selection of the most powerful predictors for the evaluation of hepatic steatosis grade: an experimental study.

Purpose: To select the most powerful predictors for the evaluation of hepatic steatosis grade.

Methods And Materials: Forty-five healthy New Zealand rabbits were randomly divided into one normal control group and three experimental groups. Hepatic steatosis models were established by feeding a high-fat, high-sugar diet and drinking water containing 5% ethanol.

Portalsystemic hemodynamic changes in chronic severe hepatitis B: An ultrasonographic study.

Aim: To evaluate portalsystemic hemodynamic changes in chronic severe hepatitis B.

Methods: Hemodynamic parameters included portal vein diameter (PVD), portal vein peak velocity (PVPV), portal vein volume (PVV), spleen length (SPL), spleen vein diameter (SPVD), spleen vein volume (SPVV) and umbilical vein recanalization. They were measured by Color Doppler ultrasonography in 36 patients with chronic severe hepatitis B, compared with 51 normal controls, 61 patients with chronic hepatitis B, 46 patients with compensable cirrhosis, and 36 patients with decompensable cirrhosis.

[Effects of beta-cypermethrin on voltage-gated potassium channels in rat hippocampal CA3 neurons].

The effects of beta-cypermethrin (consisting of alpha-cypermethrin and theta-cypermethrin) on the transient outward potassium current (I(A)) and delayed rectifier potassium current (I(K)) in freshly dissociated hippocampal CA3 neurons of rats were studied using whole-cell patch-clamp technique. The results indicated that alpha-cypermethrin increased the value of I(A) and theta-cypermethrin decreased the value of I(A), though both of them shifted steady activation curve of I(A) towards negative potential. theta-cypermethrin contributed to the inactivation of I(A).