Brujavanoids A-U, structurally diverse apotirucallane-type triterpenoids from Brucea javanica and their anti-inflammatory effects.

Extensive phytochemical investigation on the methanol extract of the inflorescences, twigs, and leaves of Brucea javanica led to the isolation and identification of 27 triterpenoids, including 21 previously undescribed ones, named brujavanoids A-U (1-21). Their structures were determined based on comprehensive spectroscopic analysis and single-crystal X-ray diffraction. Of these compounds, brujavanoid A (1) represents the first apotirucallane-type triterpenoid with a novel 19(10 → 9)abeo motif, and brujavanoids B and C (2-3) are the first apotirucallane-type triterpenoids with a rarely occurring 14-hydorxy-15,16-epoxy fragment.

Modeling and optimization of phenylalanine ammonia lyase stabilization in recombinant Escherichia coli for the continuous synthesis of l-phenylalanine on the statistical-based experimental designs.

Some approaches for improving recombinant phenylalanine ammonia lyase (PAL) stability in Escherichia coli during the enzymatic methods of l-phenylalanine (l-Phe) production were developed following preliminary studies by means of statistical-based experiment designs (response surface method). The traditional non-statistical technology was used to screen four critical factors for PAL stability during the bioconversion process, viz., glycerin, sucrose, 1,4-dithiothreitol (DTT), and MgSO(4).

(4RS)-3-Benzyl 5-methyl 2,6-dimethyl-4-(4-nitro-phen-yl)-1,4-dihydro-pyridine-3,5-dicarboxyl-ate.

In the title compound, C(23)H(22)N(2)O(6), the crystal packing is stabilized by inter-molecular N-H⋯O hydrogen bonds, which link the mol-ecules into chains running parallel to the c axis. Inter-molecular C-H⋯O hydrogen bonds are also present in the structure.

1-Phenyl-1-[(1-phenyl-ethyl)sulfonyl-methyl-sulfon-yl]ethane.

There are two mol-ecules in the asymmetric unit of the title compound, C(17)H(20)O(4)S(2). There are slight differences in the twist of the two rings relative to the S-C-S chain [dihedral angles of 48.41 (18) and 87.