[Antioxidants inhibit the oxidative modification of high density lipoproteins].

Objective: To study the role and mechanism of antioxidants on inhibiting oxidative modification of high density lipoproteins (HDL).

Methods: Freshly prepared human plasma HDL was treated by incubation with copper ion, hyperchlorite or arterial wall cells. Compared to control, the test groups were treated with addition of different concentration of butylhydroxytoluene (BHT), vitamin C and vitamin E.

[Polymorphism in beta2 adrenergic receptor gene in Chinese population with obesity].

Objective: To investigate the variation of beta2 adrenergic receptor (beta2 AR) gene and its association with obesity in Chinese population.

Methods: The allele of beta2 AR gene at Arg16Gly and Gln27Glu sites were analysed with polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) in 396 people with Han nationality in Chengdu, among whom 126 had obesity.

Results: The allele frequencies of Arg and Gly at Arg16Gly site were 0.

[Analysis of -3826A/G polymorphism in the promoter of the uncoupling protein-1 gene in Chinese non-obese and obese populations].

Objective: To investigate the -3826A/G polymorphism in the promoter of the uncoupling protein-1 (UCP1) gene and its relations to obesity in Chinese population.

Methods: Three hundred and eighty-four subjects (257 non-obese and 127 obese individuals) from a population of Chinese Han nationality in Chengdu area were studied using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs). Serum lipids were measured by enzymatic kits and apolipoproteins A I, A II, B100, C II, C III and E were measured by the RID kits.

[Association of apolipoprotein A5 gene -1131T/C polymorphism with serum lipids and carotid intima-media thickness in patients with type 2 diabetes mellitus].

Objective: To investigate the relationship between apolipoprotein A5 gene -1131T/C polymorphism and serum lipids levels and carotid intima-media thickness in patients with type 2 diabetes mellitus in a Chinese population in Chengdu.

Methods: The genotype and allele frequencies of apolipoprotein A5-1131T/C polymorphism were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polyacrylamide electrophoresis (PAGE) methods. The serum lipids were measured with enzymatic kits in 154 type 2 diabetic patients and 206 normal people (control).

Identification of a novel gene, MSAG, regulated by high levels of glucose and insulin.

Identification and characterization of novel genes involved in derangement of metabolisms of glucose and triglycerides are important in understanding the development of metabolic syndrome (MS) and atherosclerosis. Model rats with certain phenotypes of MS were fed a high-carbohydrate diet. The rat hepatic subtracted cDNA libraries were constructed and screened.

[The -384A >C polymorphism of endothelial lipase gene promoter region in Chinese healthy normolipidemic and endogenous hypertriglyceridemic subjects].

Objective: To investigate the effects of the -384A>C polymorphism in the promoter region of endothelial lipase (EL) gene on serum lipid and apolipoprotein levels in healthy normolipidemic (HTG) and endogenous hypertriglyceridemic (HTG) subjects.

Methods: Two hundred and fourteen healthy normolipidemic and 103 endogenous hypertriglyceridemic subjects from a population of Chinese Han nationality in Chengdu area were studied using restriction fragment length polymorphism (RFLPs). Serum lipids were measured by enzymatic kits and apolipoproteins AI, AII, B100, CII, CIII and E were measured by the radial immunadiffussion kits.

Distribution and effect of apo A-IV genotype on plasma lipid and apolipoprotein levels in a Chinese population.

Objectives: Hypertriglyceridaemia has been recognized as an independent risk factor for the development of coronary heart disease. Apolipoprotein A-IV (apo A-IV) plays an important role in the metabolism of TG-rich lipoproteins and HDL. However, the role of the polymorphism of the apo A-IV gene in hyperlipidaemia remains to be fully determined.

[Analysis of beta2-adrenergic receptor gene (beta2AR) Arg16Gly polymorphism in patients with endogenous hypertriglyceridemia in Chinese population].

Objective: To investigate the Arg16Gly polymorphism of beta2-adrenergic receptor (beta2AR) gene and its association with endogenous hypertriglyceridemia (HTG) in Chinese population.

Methods: Three hundred and forty one subjects including 100 HTG patients and 241 healthy controls from a population of Chinese Han nationality in Chengdu area were studied using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs).

Results: The frequencies of Gly allele at the Arg16Gly locus in combined group was 0.

[Inhibitory effect of quercetin, rutin and puerarin on LDL oxidation induced by Cu2+].

Objective: To evaluate the inhibitory effect of quercetin, rutin and puerarin on the LDL oxidation induced by Cu2+ and to investigate their action on the prevention and treatment of atherosclerosis.

Method: The serum LDL was isolated by the one step density gradient ultracentrifugation. The LDL oxidation was induced by Cu2+ in vitro for different time periods.

[Inhibitory effect of isorhamnetin and hesperidin on the oxidation of high-density lipoproteins (HDL) induced by Cu2+].

Objective: To test the inhibitory effect of isorhamnetin and hesperidin on the HDL oxidation induced by Cu2+.

Methods: The serum HDL was isolated by the one step density gradient ultracentrifugation. The HDL oxidation was induced by Cu2+ in vitro for different length of periods.

[Inhibitory effect of Isorhamnetin and Hesperidin on LDL oxidation induced by Cu2+].

Objective: To evaluate the inhibitory effect of Isorhamnetin and Hesperidin on the LDL oxidation induced by Cu2+.

Methods: The serum LDL was isolated by the one step density gradient ultracentrifugation. The LDL oxidation was induced by Cu2+ in vitro for different time periods.

[Association between cholesterol 7alpha-hydroxylase -204A/C gene polymorphism and endogenous hypertriglyceridemia in Chinese].

Objective: To investigate the cholesterol 7alpha-hydroxylase gene -204A/C polymorphism and its relationship with serum lipids and apolipoproteins (apo) levels in patients with endogenous hypertriglyceridemia (HTG) in Chinese population in Chengdu area.

Methods: The genotype and allele frequencies of cholesterol 7alpha-hydroxylase gene -204A/C polymorphism were analyzed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Serum lipids were measured by enzymatic kits and apolipoproteins AI, AII, B100, CII, CIII and E were measured by the RID kits in 132 HTG patients and 212 control subjects.

[Purification of human VLDL apolipoproteins by middle pressure liquid chromatography].

Objective: To purify human VLDL apolipoproteins by middle-pressure liquid chromatography.

Methods: Human VLDLs were isolated by one step density ultracentrifugation. Delipided human VLDL was separated by Sephacryl S-200 molecular sieve chromatography.

[Analysis of ATP binding cassette A1 gene R219K polymorphism in patients with endogenous hypertriglyceridemia in Chinese population].

Objective: To investigate the variations of ATP binding cassette A1 (ABCA1) gene and its relation to endogenous hypertriglyceridemia (HTG) in Chinese population.

Methods: A total of three hundred and nine subjects (one hundred and nine endogenous hypertriglyceridemics and 200 healthy controls) from a population of Chinese Han nationality in Chengdu area were studied using restriction fragment length polymorphism (RFLP) amplified by polymerase chain reaction (PCR).

Results: The frequency of K allele at R219K site 0.

[Analysis of cholesterol ester transfer protein gene Taq IB and -629 C/A polymorphisms in patients with endogenous hypertriglyceridemia in Chinese population].

Objective: To investigate the variations of cholesterol ester transfer protein (CETP) gene and its relation to endogenous hypertriglyceridemia (HTG) in Chinese population.

Methods: One hundred and thirty-five endogenous hypertriglyceridemics and 214 healthy subjects from a population of Chinese Han nationality in Chengdu area were studied using restriction fragment length polymorphism (RFLPs) amplified by polymerase chain reaction (PCR). The polymorphic sites studied included Taq IB and -629 C/A polymorphism in CETP gene.

Plasma very-low-density lipoprotein, low-density lipoprotein, and high-density lipoprotein oxidative modification induces procoagulant profiles in endogenous hypertriglyceridemia.

This study was to investigate whether oxidatively modified lipoproteins were associated with changes of pro- and anticoagulant profiles in hypertriglyceridemic subjects. Plasma VLDL, LDL, and HDL were isolated with the one-step density gradient ultracentrifugation method. The oxidation of the lipoproteins was identified.

[Construction and preliminary screening of a forward-subtracted cDNA library for differentially expressed genes in rat liver of prothrombotic state].

Objective: To construct and preliminarily screen the forward-subtracted cDNA library of differentially expressed genes in rat liver of prothrombotic state (PTS).

Methods: The forward-subtracted cDNA library for differentially expressed genes in rat liver of PTS was constructed by suppression subtractive hybridization using cDNAs synthesized from mRNA of PTS rat as Tester and cDNAs from mRNA of control rat as Driver. The products from the last PCR amplification of suppression subtractive hybridization were inserted into a T/A plasmid vectors to transform the Escherichia coli JM109 cells.

[The effect of high density lipoprotein and oxidized high density lipoprotein on some secretive functions of endothelial cells].

Objective: To investigate the effect of high density lipoprotein and oxidized high density lipoprotein on the synthesis of NO, the activity of NOS, and the secretion of tPA and PAI-1 by cultured human umbilical vein endothelial cells (HUVEC).

Methods: With the 0. 01 mol/L PBS and N-HDL of 50 mg/L as control, OX-HDL of 5 mg/L,10 mg/L, 50 mg/L were co-incubated with HUVEC for 24 h in vitro.

[Screening of prothrombotic state-related genes from human hepatic cDNA library].

Objective: To screen prothrombotic state-related cDNA sequences from human hepatic cDNA library.

Methods: The subtracted cDNA library for differentially expressed genes in rat liver of prothrombotic state (PTS) was constructed by suppression subtractive hybridization. Positive clones were identified by differential screening.

[Cloning and bioinformatic analysis of a novel gene HCR2 up-regulated in aorta of hypercoagulable rat].

Objective: The aim of this study was to clone the full-length cDNA of HCR2 up-regulated in aorta of hypercoagulable rat and make the relevant bioinformatic analysis.

Methods: Rapid amplification of cDNA end (RACE) and nested PCR technique was used to amplify the full-length cDNA of HCR2 from an EST (GenBank accession number BQ901227) which was significantly up-regulated in hypercoagulable rat.

Results: The full-length cDNA of HCR2 has been obtained (GenBank accession number AY234417).

Identification of a novel gene, HCR2, in aorta of hypercoagulable rats using suppression subtractive hybridization.

Screening and identification of novel genes involved in hypercoagulable state (HCS) is important in understanding the underlying mechanisms of development of atheroslerosis, which implicated in critical vascular diseases such as coronary heart disease and stroke. HCS was induced in rats with high carbohydrate diet. Subtractive hybridization experiments between aorta tissues from hypercoagulable rats and controls showed that a novel cDNA (GenBank Accession No.

[Purification of human serum apolipoprotein A I and A II by middle-pressure liquid chromatography].

Objective: This study is made for the purification of human HDL apolipoproteins by middle-pressure liquid chromatography (MPLC).

Methods: Human HDL was isolated by one step density ultracentrifugation. Delipided human HDL was separated by Sephacryl S-200 molecular sieve chromatography.

[Study on apoE gene polymorphism and subclasses of serum high density lipoprotein in type IV hyperlipidemia].

Objective: The aim of the study was to investigate apolipoprotein(apo) E polymorphism and its relationship with serum lipids and apolipoprotein, serum high density lipoprotein(HDL) subclasses in patients with type IV hyperlipidemia.

Methods: apoE genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The subclasses of serum HDL in 103 patients with type IV hyperlipidemia and 146 normolipidemic subjects were determined by two-dimensional gel electrophoresis in conjunction with immunodetection method.