[Hereditary quality standards for laboratory fish].

Although laboratory fish are increasingly used in genetics and other life science research fields, standard quality control and supervision are needed. In China, laboratory animals are all put into a strict licensing and quality management system by the government. The standardization of genetic quality control is crucial to a laboratory fish quality control management system.

[Development and application of TaqMan MGB probe fluorescence quantitative PCR method for rapid detection of Clostridium piliforme].

Objective: To develop a TaqMan MGB probe-based, sensitive and specific fluorescence quantitative PCR assay method for rapid detection of Clostridium piliforme.

Methods: Primers and probes specific to 16S rRNA gene of Clostridium piliforme were designed. A TaqMan MGB probe-based, fluorescence quantitative PCR method was established.

[Microsatellite analysis in Wistar and Spague-Darley outbred rats].

Genetic quality of outbred stock has an important effect for the experimental results by using those animals, but methods and standardization of genetic detection for outbred stock are absent. In the present study, 6 microsatellite markers were screened for Wistar from Beijing and Spague-Darley (SD) from Shanghai outbred rats by fluorescence-based semi-automated genotyping method. Good polymorphisms were detected on all the 6 microsatellite loci, with 36 alleles found in the 2 stocks, 5-8 alleles each locus, and the polymorphic information content (PIC) ranged from 0.