Publications by authors named "Bing-Cun Ma"
Article Synopsis
- Researchers studied lactic acid bacteria (LAB) in Tibetan chickens to find safe strains that could deliver foreign proteins for vaccines.
- They identified a strain called Lactobacillus salivarius TCMM17, which strongly adheres to chicken gut cells and shows no harmful effects or resistance to antibiotics.
- By inserting a specific gene into TCMM17, they created a modified strain that can maintain this gene over generations and display the related protein on its surface, paving the way for new oral vaccines against bird diseases.
Objective: To assemble infectious bronchitis virus (IBV)-like particles bearing the recombinant spike protein and investigate the humoral immune responses in chickens.
Results: IBV virus-like particles (VLPs) were generated through the co-infection with three recombinant baculoviruses separately encoding M, E or the recombinant S genes. The recombinant S protein was sufficiently flexible to retain the ability to self-assemble into VLPs.
An indirect enzyme-linked immunosorbent assay (ELISA) method based on a novel multi-epitope antigen of S protein (SE) was developed for antibodies detection against infectious bronchitis virus (IBV). The multi-epitope antigen SE protein was designed by arranging three S gene fragments (166-247 aa, S1 gene; 501-515 aa, S1 gene; 8-30 aa, S2 gene) in tandem. It was identified to be approximately 32 kDa as a His-tagged fusion protein and can bind IBV positive serum by western blot analysis.
View Article and Find Full Text PDFInfectious bronchitis virus (IBV) causes tremendous economic losses to the poultry industry. Here, we report the complete genome analysis results for a new natural recombination nephropathogenic IBV strain named SAIBK, which was isolated in the Sichuan province of China in 2005.
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