New approach to control ischemic severity ex vivo.

Background: It is advantageous to be able to both control and define a metric for ischemia severity in ex vivo models to enable more precise comparisons to in vivo models and to facilitate more sophisticated mechanistic studies. Currently, the primary method to induce and study ischemia ex vivo is to completely deplete oxygen and glucose in the culture media; however, in vivo ischemia often involves varying degrees of severities.

New Method: In this work, we have successfully developed an approach to both control and characterize three different ischemic severities ex vivo and we define these standard condition metrics via an oxygen sensor: normoxia (control), mild ischemia (partial oxygen-glucose deprivation), and severe ischemia (complete oxygen-glucose deprivation).

Dynamic and Rapid Detection of Guanosine during Ischemia.

Guanosine acts in both neuroprotective and neurosignaling pathways in the central nervous system; in this paper, we present the first fast voltammetric measurements of endogenous guanosine release during pre- and post-ischemic conditions. We discuss the metric of our measurements via analysis of event concentration, duration, and interevent time of rapid guanosine release. We observe changes across all three metrics from our normoxic to ischemic conditions.

Tinospora cordifolia (Willd.) Miers: phytochemical composition, cytotoxicity, proximate analysis and their biological activities.

The present research work has been performed to evaluate the phenolic content, flavonoids content, and cytotoxicity of a multidimensional medicinal plant; Tinospora cordifolia and as well as to determine nutritive value by proximate analysis. The total phenolic and flavonoids contents of Tinospora cordifolia were found to be significantly greater in methanol extract as compared to corresponding hexane extract. Brine shrimp bioassay indicated Tinospora cordifolia is pharmacologically active.

Current Trends of Food Analysis, Safety, and Packaging.

Food is a basic necessity for life, growth, survival, and maintaining a proper body function. Rising food demand leads both producers and consumers to search for alternative food sources with high nutritional value. However, food products may never be completely safe.

The impact of COVID-19 outbreak and perceptions of people towards household waste management chain in Nepal.

The spread of COVID-19 is posing significant challenges to the household (HH) waste management sectors putting waste personnel and concerned bodies under massive pressure. The chain of collection, segregation, recycling, and disposal of household generated wastes is interrupted. This study aimed to assess how the household waste management chain was disrupted by novel coronavirus in Nepal and find the perception of the people towards the existing household waste management system (HHWMS).