ALIBY: ALFA Nanobody-Based Toolkit for Imaging and Biochemistry in Yeast.

Specialized epitope tags continue to be integral components of various biochemical and cell biological applications such as fluorescence microscopy, immunoblotting, immunoprecipitation, and protein purification. However, until recently, no single tag could offer this complete set of functionalities on its own. Here, we present a plasmid-based toolkit named ALIBY (FA toolkit for maging and iochemistry in east) that provides a universal workflow to adopt the versatile ALFA tag/ALFA system within the well-established model organism Saccharomyces cerevisiae.