Potential utility of callus proteases as a milk clotting alternative to naturally propagated proteases.

This study was intended to understand suitability of calli proteases (CCE) as vegetable coagulant through delineating their milk clotting potential and casein (whole and κ-casein) hydrolysis pattern in comparison to their plant stem crude enzyme (PSCE), rennin and Enzeco (positive controls). Stem calli was induced using two concentrations of naphthalene acetic acid (NAA) [0.5 mg/L (M1), 1 mg/L (M2)] with fixed concentration (1 mg/L) of 6-benzylaminopurine (BAP).

Purification, characterization and fibrino(geno)lytic activity of cysteine protease from latex.

Protease was isolated and purified from latex and its hemostatic potential was analyzed. Crude latex enzyme was purified through ion exchange and gel filtration chromatography. Purified protease was characterized and its thrombin-like (coagulant assay, fibrinogen polymerizing, and fibrinogenolytic activity) and plasmin-like (blood and plasma clot lysis) activities were assessed accordingly.

Three phase partitioning to concentrate milk clotting proteases from Wrightia tinctoria R. Br and its characterization.

Wrightia tinctoria stem proteases were partially purified for the first time through a non-chromatographic technique, three phase partitioning (TPP), to concentrate the milk clotting proteases. Various parameters like salt and solvent concentration that affect the partitioning of the protease were examined. Maximum recovery and purification fold of the protease activity were found in the interfacial phase (IP) with 60% ammonium sulphate and 1:1 crude enzyme to t-butanol.

Clinical and diagnostic utility of saliva as a non-invasive diagnostic fluid: a systematic review.

This systematic review presents the latest trends in salivary research and its applications in health and disease. Among the large number of analytes present in saliva, many are affected by diverse physiological and pathological conditions. Further, the non-invasive, easy and cost-effective collection methods prompt an interest in evaluating its diagnostic or prognostic utility.

Hemostatic, milk clotting and blood stain removal potential of cysteine proteases from Calotropis gigantea (L.) R. Br. Latex.

Introduction: Plant latex is a natural source of biologically active compounds and several hydrolytic enzymes responsible for their diverse health benefits. Recent past has witnessed substantial progress in understanding their supplementary industrial and pharmaceutical utility. Calotropis gigantea is one of the important latex producing plants belonging to asclepediaceae family with wide ethnopharmacological applications and is rich in proteolytic enzymes.