Publications by authors named "Bindhu K Madhavan"
Article Synopsis
- The ARP2/3 complex plays a crucial role in the organization of the actin cytoskeleton and the formation of structures called lamellipodia, which are important in the progression of pancreatic ductal adenocarcinoma (PDAC).
- This study investigates how the ARP2/3 complex affects cancer-associated fibroblasts (CAFs) that contribute to PDAC advancement and patient survival, using mouse models and human cell studies.
- Results indicate that the ARP2/3 complex is vital for CAF migration and differentiation, suggesting that targeting it could be an effective new approach for treating PDAC.
An elevated frequency of DNA replication defects is associated with diabetes and cancer. However, data linking these nuclear perturbations to the onset or progression of organ complications remained unexplored. Here, we report that RAGE (Receptor for Advanced Glycated Endproducts), previously believed to be an extracellular receptor, upon metabolic stress localizes to the damaged forks.
View Article and Find Full Text PDFStable cell cloning is an essential aspect of biological research. All advanced genome editing tools rely heavily on stable, pure, single cell-derived clones of genetically engineered cells. For years, researchers have depended on single-cell dilutions seeded in 96- or 192-well plates, followed by microscopic exclusion of the wells seeded with more than or without a cell.
View Article and Find Full Text PDFWith the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for inducing, testing, and studying the repair mechanisms associated with DNA double-strand breaks, interstrand cross-link repair, and single-strand break repair.
View Article and Find Full Text PDFArticle Synopsis
- Small cell lung carcinoma (SCLC) is a very aggressive cancer associated with high mortality rates and poor response to traditional chemotherapies like etoposide and cisplatin.
- Recent findings indicate that an elevated expression of a specific variant in SCLC enhances the repair of DNA damage caused by anti-cancer drugs, helping tumor cells survive treatment.
- This variant operates in the nucleus and aids in recruiting DNA repair factors, suggesting it could be a promising target for new therapies aimed at improving treatment outcomes for advanced SCLC.