Challenges and Opportunities of Uranium Extraction From Seawater: a Systematic Roadmap From Laboratory to Industry.

Uranium extraction from seawater (UES) is crucial for ensuring the sustainable development of nuclear power and has seen significant advancements in recent years. However, natural seawater is a highly complex biogeochemical system, characterized by an extremely low uranium (U) concentration (≈3.3 µg L), abundant competitive ions, and significant marine biological pollution, making UES a formidable challenge.

Loss of gdnfa disrupts spermiogenesis and male courtship behavior in zebrafish.

Spermatogenesis is essential for establishment and maintenance of reproduction in male vertebrates. Spermatogenesis, which is mainly regulated by the combined action of hormones, growth factors, and epigenetic factors, is highly conserved. Glial cell line-derived neurotrophic factor (GDNF) is a member of the transforming growth factor-β superfamily.

Overexpression of improved hepatic glucose catabolism and relieved liver lipid deposition in common carp ( L.) fed a high-starch diet.

Growth hormone (GH) is important for regulating insulin secretion and carbohydrate metabolism, and its role in mammalian models of diabetes is relatively worked out. Although some fish species were used as models for diabetes research, the effects of GH on insulin and glucose catabolism and anabolism in these models remain to be clarified. In this study, we investigated the effect of GH on insulin and glucose catabolism and anabolism in an omnivorous fish using transgenic (T) common carp that consistently overexpressed and wild-type (WT) common carp.

Improved liver lipid catabolism and utilization in transgenic common carp ( L.) through enhanced lipolytic and fatty acid β-oxidation pathways.

() transgenic common carp ( L.) show desirable aquaculture traits. Their specific growth rate (SGR) and feed efficiency (FE) are approximately 12% and 17% higher than the wild-type (WT) common carp, respectively.

Effects of Secretoneurin and Gonadotropin-Releasing Hormone Agonist on the Spawning of Captive Greater Amberjack ().

The greater amberjack (), a pelagic marine species with a global distribution, has considerable worldwide potential as an aquaculture species. However, difficulties have been encountered in inducing spontaneous spawning in cultured fish stocks. In this study, we analysed the key regulatory factors, secretoneurin (SN) and gonadotropin-releasing hormone (GnRH), in greater amberjack.

A novel electrochemical sensor based on N, S co-doped liquorice carbon/functionalized MWCNTs nanocomposites for simultaneous detection of licochalcone A and liquiritin.

In this study, nanoporous C was prepared from the roots, stems and leaves of liquorice and modified via element doping. Then, the nitrogen and sulfur co-doped liquorice carbon (N, S-LC) and functional multiwall carbon nanotubes (f-MWCNTs) were dispersed using an ultrasonic dispersion technology, and a binary nanocomposite was prepared. N, S-LC plays a key role in the formation and electrochemical efficiency of binary nanocomposites, and electrochemical impedance spectroscopy indicates that the binary complexes formed by the hybridisation of N, S-LC and f-MWCNTs can improve the electron transfer ability of an electrode.

Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic .

Germline stem cells (GSCs) are a group of unique adult stem cells in gonads that act as important transmitters for genetic information. Donor GSCs have been used to produce offspring by transplantation in fisheries. In this study, we successfully isolated and enriched GSCs from the ovary, ovotestis, and testis of , one of the most important breeding freshwater fishes in China.

Sinhcaf-dependent histone deacetylation is essential for primordial germ cell specification.

Primordial germ cells (PGCs) are the progenitor cells that give rise to sperm and eggs. Sinhcaf is a recently identified subunit of the Sin3 histone deacetylase complex (SIN3A-HDAC). Here, we provide evidence that Sinhcaf-dependent histone deacetylation is essential for germ plasm aggregation and primordial germ cell specification.

Dynamic Gene Expression and Alternative Splicing Events Demonstrate Co-Regulation of Testicular Differentiation and Maturation by the Brain and Gonad in Common Carp.

The common carp () accounts for approximately 10% of the annual freshwater aquaculture production and is an ideal model to study cyprinidae reproduction. Female common carp grow faster than the males; therefore, related research presents an opportunity with high application value. Although we have a detailed understanding of common carp's early gonadal differentiation process, information about genome-wide gene expression, regulation, and underlying molecular mechanisms during this process remain limited.

Associations Between Oral Health Status, Perceived Stress, and Neuropsychiatric Symptoms Among Community Individuals With Alzheimer's Disease: A Mediation Analysis.

Community individuals with Alzheimer's disease (AD) experience oral disease alongside neuropsychiatric symptoms (NPS) with disease progression. Despite growing evidence for the link between oral health and cognitive status, few studies have investigated the associations between oral health and NPS, especially based on individuals' experience of AD. The primary aim of this study was to examine (a) the difference in oral health-related stressors among individuals with AD, mild cognitive impairment (MCI), and subjective cognitive decline (SCD); and (b) the associations of these stressors with NPS under the framework of the stress process model (SPM).

Multiple Developmental Defects in Mutant Zebrafish with Features of Coffin-Siris Syndrome.

A previous study suggested that human Coffin-Siris syndrome is related to the mutation of . Since the homozygous mutant mice died soon after birth, no suitable model was available for the study of the pathogenic mechanism of Coffin-Siris syndrome. To solve this problem, we generated two viable homozygous zebrafish mutants, and .

Secretoneurin is a secretogranin-2 derived hormonal peptide in vertebrate neuroendocrine systems.

Secretogranin-2 (SCG2) is a large precursor protein that is processed into several potentially bioactive peptides, with the 30-43 amino acid central domain called secretoneurin (SN) being clearly evolutionary conserved in vertebrates. Secretoneurin exerts a diverse array of biological functions including regulating nervous, endocrine, and immune systems in part due to its wide tissue distribution. Expressed in some neuroendocrine neurons and pituitary cells, SN is a stimulator of the synthesis and release of luteinizing hormone from both goldfish pituitary cells and the mouse LβT2 cell line.

Dynamics Analysis and Design for a Bidirectional Super-Ring-Shaped Neural Network With n Neurons and Multiple Delays.

Recently, the dynamics of delayed neural networks has always incurred the widespread concern of scholars. However, they are mostly confined to some simplified neural networks, which are only made up of a small amount of neurons. The main cause is that it is difficult to decompose and analyze generally high-dimensional characteristic matrices.

Targeted mutation of secretogranin-2 disrupts sexual behavior and reproduction in zebrafish.

The luteinizing hormone surge is essential for fertility as it triggers ovulation in females and sperm release in males. We previously reported that secretoneurin-a, a neuropeptide derived from the processing of secretogranin-2a (Scg2a), stimulates luteinizing hormone release, suggesting a role in reproduction. Here we provide evidence that mutation of the and genes using TALENs in zebrafish reduces sexual behavior, ovulation, oviposition, and fertility.

A novel fluorescent probe based on naphthalimide for imaging nitroreductase (NTR) in bacteria and cells.

A nitroreductase (NTR) responsive fluorescent probe, Na-NO, comprising p-nitrobenzyl as the unique recognition group and 1,8-naphthalimide as fluorophore, was synthesized. Na-NO showed remarkable fluorescence "turn-on" signal in the presence of NTR under DMSO/HO (1:19, v/v) buffered with PBS (pH = 7) solution in the presence of NADH (300 µM). Furthermore, the probe has a low detection limit down to 3.

GSH-responsive anti-mitotic cell penetrating peptide-linked podophyllotoxin conjugate for improving water solubility and targeted synergistic drug delivery.

Podophyllotoxin (PPT) is a chemotherapeutic agent which has shown significant anti-cancer effects through inhibiting microtubule assembly. However, because of the poor water solubility and obvious side effects, PPT cannot be used in clinical cancer therapy. In order to solve these problems, a novel glutathione-responsive PPT conjugate has been synthesized in which PPT was linked to an anti-mitotic cell penetrating peptide (PRA) via a disulfide linkage.

Growth Hormone Overexpression Disrupts Reproductive Status Through Actions on Leptin.

Growth and reproduction are closely related. Growth hormone (GH)-transgenic common carp exhibit accelerated growth and delayed reproductive development, which provides an amenable model to study hormone cross talk between the growth and reproductive axes. We analyzed the energy status and reproductive development in GH-transgenic common carp by using multi-tissue RNA sequencing, real-time-PCR, Western blotting, ELISA, immunofluorescence, and incubation.

Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish ().

The main reason for abnormal development of cloned animals or embryos, and inefficient animal cloning, is a poor understanding of the reprogramming mechanism. To better comprehend reprogramming and subsequent generation of pluripotent stem cells, we must investigate factors related to reprogramming of somatic cells as nuclear donors. As we know, (family with sequence similarity 60, member A, like) is a coding gene only found in zebrafish and frog () among vertebrates.

Mutation Disrupts Gamete Maturation and Reduces Fertility in Zebrafish.

N-methyladenosine (mA), catalyzed by Mettl3 methyltransferase, is a highly conserved epigenetic modification in eukaryotic messenger RNA (mRNA). Previous studies have implicated mA modification in multiple biological processes, but the function of mA has been difficult to study, because mutants are embryonic lethal in both mammals and plants. In this study, we have used transcription activator-like effector nucleases and generated viable zygotic mutant, Z , in zebrafish.

GABAergic Neurons and Their Modulatory Effects on GnRH3 in Zebrafish.

γ-Aminobutyric acid (GABA) is a major amino acid neurotransmitter in the vertebrate brain. To provide detailed information on the distribution of the GABA in zebrafish (Danio rerio), neurons were labeled with mCherry driven by the glutamic acid decarboxylase 67 (gad67) promoter. In the transgenic line Tg(gad67:mCherry), mCherry-positive gad67 cell bodies were predominantly localized to the olfactory bulb, pallial zones, subpallium zones, parvocellular preoptic nucleus, periventricular gray zone of optic tectum, torus semicircularis, posterior tuberculum, medial longitudinal fascicle, caudal zone of periventricular hypothalamus, and oculomotor nucleus.