Whole-Genome Resequencing Reveals Signatures of Adaptive Evolution in and .

and are economically important marine species along the coast of China, with similar external morphological characteristics and living habits, with wide distribution and strong adaptability. To investigate the molecular mechanisms underlying the adaptive evolution of these two species, we conducted whole-genome resequencing of 10 individuals of both species from the coastal waters of Wuyu Island, Fujian, China, using high-throughput sequencing technology. We obtained SNP, InDel, CNV, and SV variation information and annotated these variations, constructing a genomic variation database for both species.

Transcriptome Responses to Different Environments in Intertidal Zones in the Peanut Worm .

The peanut worm () is an important intertidal species worldwide. Species living in the same aquaculture area might suffer different environmental impacts. To increase knowledge of the molecular mechanisms underlying the response to environmental fluctuations, we performed a transcriptome analysis of from different intertidal zones using a combination of the SMRT platform and the Illumina sequencing platform.

Comparative transcriptome sequencing analysis of female and male .

Sexual growth dimorphism is a common phenomenon in teleost fish and has led to many reproductive strategies. Growth- and sex-related gene research in teleost fish would broaden our understanding of the process. In this study, transcriptome sequencing of shortfin scad was performed for the first time, and a high-quality reference transcriptome was constructed.

The complete mitochondrial genome and phylogenetic analysis of the ocellated angelshark: Chen, 1963.

The ocellated angelshark ( Chen, 1963) is a threatened shark within the family Squatinidae. In the present study, we reported the mitochondrial genome sequence of the ocellated angelshark. The complete mitochondrial genome is 16,683 bp in length and contains 37 mitochondrial genes and a control region as similar to most fishes.

Spatial Distribution, Potential Risks and Source Identification of Heavy Metals in the Coastal Sediments of the Northern Beibu Gulf, South China Sea.

Thirty samples of surface sediments (0-5 cm) from the northern Beibu Gulf were analyzed to determine the spatial distribution, potential risks and sources of six heavy metals (Cr, Cu, Zn, As, Cd and Pb). The concentrations (mg/kg, dw) of Cr, Cu, Zn, As, Cd and Pb were 15.38 ± 6.

Complete Mitochondrial Genome of Two Ectoparasitic Capsalids (Platyhelminthes: Monogenea: Monopisthocotylea): Gene Content, Composition, and Rearrangement.

The capsalid monogeneans are important pathogens that generally infect marine fishes and have a substantial impact on fish welfare in aquaculture systems worldwide. However, the current mitogenome information on capsalids has received little attention, limiting the understanding of their evolution and phylogenetic relationships with other monogeneans. This paper reports the complete mitochondrial genomes of and for the first time, which we obtained using a next-generation sequencing method.

Transcriptomic analysis of male three-spot swimming crab (Portunus sanguinolentus) infected with the parasitic barnacle Diplothylacus sinensis.

Diplothylacus sinensis is reported as an intriguing parasitic barnacle that can negatively affect the growth, molting, reproduction in several commercially important portunid crabs. To better understand the molecular mechanisms of host-parasite interactions, we characterized the gene expression profiles from the healthy and D. sinensis infected Portunus sanguinolentus by high-through sequence method.

Efficacy of EGFR-TKI sequential therapy in patients with exon 19 insertion-positive non-small-cell lung cancer: A case report.

Background: Insertions in exon 19 in the epidermal growth factor receptor gene (EGFR) is a rarely seen mutation in non-small cell lung cancer. These patients have been effectively treated with sequential EGFR tyrosine kinase inhibitors (TKIs).

Case Summary: Here, we presented a case of non-small cell lung cancer, stage IIIB, with exon 19 insertion mutation as detected in the right lower lobe by next-generation sequencing.

Next generation sequencing yields the complete mitochondrial genome of the darkfin hind (Serranidae) from the Mischief Reef, South China Sea.

The complete mitochondrial genome of the (Serranidae: Epinephelinae) was sequenced by next-generation sequencing method on the basis of one female specimen collected from coral reef areas at the Mischief Reef, South China Sea. The mitogenome is 16,593 bp in length, including 13 protein-coding genes, 22 tRNA genes, two rRNA genes and a D-loop region. The overall content of A + T is 55.

The complete mitochondrial genome and phylogenetic analysis of the reticulated swellshark: Chan, 1966.

In the present study, the complete mitogenome of has been sequenced and assembled. The complete mitochondrial genome is 16,509 bp in length and contains 37 mitochondrial genes and a control region as other fishes. The nucleotide composition of .

De novo assembly and annotation of the whole transcriptome of Muraenesox cinereus.

In this study, we generated the transcriptome of Muraenesox cinereus from four combined tissues (muscle, sexual, liver and heart) using high-seq sequencing technology. De novo assembly was performed using Trinity software and a total of 62,125,296 high-quality clean reads were assembled and clustered into 75,862 unigene with an N50 of 2034 nt. After annotation, 43,157 unigenes had significant hit in Nr database.

Identifying Genomic Alterations in Small Cell Lung Cancer Using the Liquid Biopsy of Bronchial Washing Fluid.

With the rapid development of cancer genomics and immunomics, some new treatments of small cell lung cancer (SCLC) are emerging. However, there are limitations to the clinical use of tumor tissue. Our study aimed to evaluate the potential use of bronchial washing fluid (BWF) in the liquid biopsy of SCLC.

The complete mitochondrial genome of the (Platyhelminthes, Monogenea), a pathogen of the sailfish ().

This study reports the complete mitochondrial genome of the (Monogenea: Capsalidae) collected from the gill lamella of . The total length of the mitogenome was 13,948 bp, containing 12 typical platyhelminthic protein-coding genes, 22 tRNA genes, 2 rRNA genes and a putative non-coding region, with the gene being absent. The total A + T content was 65.

Heavy metal pollution and stable isotope ratios (δC and δN) in marine organisms from the Northern Beibu Gulf, South China Sea.

Twenty biota species were collected from the Northern Beibu Gulf to understand the heavy metal pollution status and biomagnification characteristics. Mean concentrations (μg/g) of Mn, Zn, Pb, Cr, Ni, As, Cu, and Cd in the biota species were 0.99, 38.

Comparative transcriptomic analysis for identification of candidate sex-related genes and pathways in Crimson seabream (Parargyrops edita).

Teleost fishes display the largest array of sex-determining systems among animals, resulting in various reproductive strategies. Research on sex-related genes in teleosts will broaden our understanding of the process, and provide important insight into the plasticity of the sex determination process in vertebrates in general. Crimson seabream (Parargyrops edita Tanaka, 1916) is one of the most valuable and abundant fish resources throughout Asia.

Heavy metal concentrations and associated health risks in edible tissues of marine nekton from the outer Pearl River Estuary, South China Sea.

Seven heavy metals including Hg, Cu, Pb, Cd, Zn, Cr, and As were examined in seventeen marine nekton species from the outer Pearl River Estuary (PRE), South China Sea. On the wet weight basis, the metal concentration ranges were 0.016-0.

The complete mitochondrial genome of milk shark, (Ruppell 1837).

The complete mitogenome sequence of the milk shark, of which was determined using long PCR. The genome was 16,693 bp in length and it contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 D-loop region. The overall base composition of the heavy strand is A (31.

Genetic effects of released swimming crab (Portunus trituberculatus) on wild populations inferred from mitochondrial control region sequences.

Along the coast of Shandong Province in China, an extensive hatchery-release programme has been conducted for more than 10 years. However, no information has been reported concerning the long-term effects on the wild population resulting from the release of large numbers of juveniles in Portunus trituberculatus. In this study, sequence variation of swimming crab P.

The complete mitochondrial genome of Nibea coibor (Perciformes: Sciaenidae).

In this study, the complete mitochondrial genome (mitogenome) sequence of Nibea coibor has been determined by long polymerase chain reaction and primer walking methods. The complete mitochondrial genome is 16,509 bp in length and contains 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA (rRNA), 22 transfer RNA (tRNA)) and a control region as other bony fishes. Within the control region, we identified the termination-associated sequence domain (TAS), the central conserved sequence block domains (CSB-F, CSB-E, CSB-D and CSB-C), and the conserved sequence block domains (CSB-2, CSB-3).