[The effect of Dermatophagoides pteronyssinus group 2 allergen T cell fusion epitope on STAT6 signaling in mice with asthma].

Objective: To investigate the alterations of signal transducer and activator of transcription factor 6(STAT6) signaling in a mouse model of asthma receiving treatment with Dermatophagoides pteronyssinus group 2 allergen(Der p 2) T cell fusion epitope and the mechanisms of the specific immunotherapy.

Methods: Thirty mice were randomly divided into three groups by the random number table method: the asthma group, the treatment group receiving immunotherapy with Der p 2 T cell fusion epitope, and the negative control group (PBS group)(n = 10 in each group). Mice in the asthma and the treatment groups received intraperitoneal (i.

[A specific immune therapeutic effect of Der p2 T cell epitope vaccine on asthma mice].

Objective: To investigate the specific immune therapeutic effect of the T cell fusion peptide vaccine from group II allergens from (Der p2).

Methods: Thirty mice were randomly divided into three groups, namely a negative control group (a PBS group), positive control group (an asthma group) and protein Der p2 T cell fusion epitope for specific immunotherapy (SIT) group (a Der p2 T group). The extract of house dust mites (HDM) was used to establish the asthmatic models in BALB/c mice, and the PBS group was always used with PBS buffer.

[ and its hypopus found in feed of ].

Objective: To observe the and its hypopus in feed of .

Methods: Feed samples were collected from farms, and and its hypopus were separated from the samples by the vibration sieve method and floating method. The glass specimens were prepared and and its hypopus were observed under an optical microscope.

[Investigation of Acaroid mites breeding in university canteen condiments].

Objective: To investigate the condition of Acaroid mite breeding in university canteen common condiments.

Methods: Thirteen kinds of condiments of the public cafeteria and 29 kinds of condiments of the canteen warehouse storage were collected. The mites were separated by the method of water film microscopy and an electric powder set, and then the film production; mite identification and counting were performed under a microscope.

[Investigation on species and community ecology of Acaroid mites breeding in stored traditional Chinese animal medicinal materials].

Objective: To investigate the species of Acaroid mites breeding in the stored traditional Chinese animal medicinal materials and the relationship between its community and habitats.

Methods: A total of 30 samples of traditional Chinese animal medicinal herbs were collected from Wuhu City, Anhui Province, China. The mites were isolated by the directly microscopic and floatation microscopic examinations, and then identified and counted under a light microscope.

[Investigation of natural foci of Metorchis taiwanensis in Wuhu area].

Objective: To explore the existence of natural foci of Metorchis taiwanensis in Wuhu area.

Methods: The ecological environment and daily living habits of residents were investigated; the eggs of M. taiwanensis in the dung of ducks in local region were collected by the sedimentation method; the Parafossarulus striatulus were disposed by the tabletting method and examined with a microscope to isolate the rediae and cercariae; the metacercariae in Pseudorasbora parve were isolated by using the homogenation.

[Immunotherapy effect of ploypeptide vaccine of allergen group 1 from Dermatophagoides farinae on asthma mice].

Objective: To investigate the enzymatic hydrolysates of ProDer f land their hydrolytic products for specific immunotherapy.

Methods: The asthma models of mice made by ProDer f 1 allergen were treated by using two kinds of hydrolysates as vaccine for analyzing their effects of immunotherapy. Fifty female BALB/c mice were randomly divided into 5 groups (n = 10 for each), i.

[Morphology observation of Histiostoma feroniarum hypopus].

Objective: To observe the external morphology of Histiostoma feroniarum hypopus under light microscope.

Methods: The samples were collected in a mushroom cultivation base, and the Histiostoma feroniarum hypopus was isolated and purified. The slide samples were prepared and observed under an optical microscope.

[Cloning and Prokaryotic Expression of Fusion Gene of Group II Allergen Der p2 T Cell Epitope from Dermatophagoides pteronyssinus].

Objective: To express and purify the T cell epitope fusion peptide of the major allergen Der p2 from Dermatophagoides pteronyssinus.

Methods: Nucleotide sequences reported to encode four T-cell epitopes (T1-T4) of Der p2 of D. pteronyssinus were linked in the rank of T1-T2-T3-T4.

Purification and antioxidant activities of baicalin isolated from the root of huangqin (Scutellaria baicalensis gcorsi).

Baicalin is a flavonoid from the root of huangqin (Scutellaria baicalensis gcorsi, a kind of Traditional Chinese Medicine and food condiment) with two pro phenolic hydroxyls. In this manuscript, high purity of baicalin (95.5 %) was isolated from the root of huangqin and its antioxidant activities were investigated.