Ectopic expression of testis-specific transcription elongation factor in driving cancer.

The testis-specific BET protein BRDT structurally resembles the ubiquitous BRD4 and is misexpressed in cancer, and we show that BRDT misexpression may affect lung cancer progression. BRDT knockdown in lung cancer cells slowed tumor growth and prolonged survival in a xenograft model. Comparative characterization of PTEFb complex participation and chromatin binding indicates BRD4-redundant and BRD4-distinct BRDT functions.

Ultrasound-triggered lysosomal alkalinization to block autophagy in tumor therapy.

Lysosomes play a crucial role in regulating cancer progression and drug resistance. However, there is a pressing need for the development of drugs that can safely and effectively modulate the pH of cancerous lysosomes in a controlled manner. In this study, we propose a novel strategy for lysosomal alkalinization triggered by piezoelectricity.

Dissecting Causal Relationship Among Immune Cells, Plasma Metabolites and Coronary Atherosclerosis: A Mendelian Randomization Study.

Background: Circulating immune cells and metabolites are linked to coronary atherosclerosis, but the specific causal relationships and the role of metabolites as mediators remain unclear.

Methods: Summary statistics from GWAS datasets on immune cells (n=3,757), circulating metabolites (n=8,299), and coronary atherosclerosis (cases n=51,589; controls n=343,079) were analyzed using bidirectional Mendelian randomization. Two-step and multivariate Mendelian randomization were employed to identify mediating metabolites, with inverse variance weighting (IVW) as the primary method.

Design, Synthesis and Bioactive Evaluation of Topo I/ Dual Inhibitors to Inhibit Oral Cancer via Regulating the PI3K/AKT/NF-κB Signaling Pathway.

The significantly rising incidence of oral cancer worldwide urgently requires the identification of novel, effective molecular targets to inhibit the progression of malignancy. DNA topoisomerase I (Topo I) is a well-established target for cancer treatment, and many studies have shown that different cancer cell genes could be targeted more selectively with one type of Topo I inhibitor. In this report, a new scaffold pyridothieno[3,2-]isoquinoline 11,11-dioxide was designed via the combination of the key fragment or bioisoster of Topo I inhibitor azaindenoisoquinolines and G-quadruplex binder quindoline.

Mucoadhesive-to-Mucopenetrating Nanoparticles for Mucosal Drug Delivery: A Mini Review.

Mucosal tissue acts as a barrier between the human body's internal environment and the external world. The mucosal tissue is shielded from injurious environmental chemicals, toxins, and pathogens by a mucus layer lining above the mucosal tissue, and meanwhile the periodic mucosal clearance accelerates the removal of mucoadhesive components. And therefore, transmucosal drug delivery is limited.

Optimal extent of left recurrent laryngeal nerve para-lymph node dissection in esophageal squamous cell carcinoma: a retrospective analysis based on cytokeratin 19 immunopositivity for micrometastasis detection.

No consensus has been achieved for the optimal extent of left recurrent laryngeal nerve para-lymph node dissection (LRLN-LND) for esophageal squamous cell carcinoma (ESCC). Surgeons follow different extents according to their experience, technical proficiency, and habits. Immunohistochemical (IHC) analysis is a feasible method to detect micrometastasis (MM) of lymph nodes in ESCC.

Antibiotics in the treatment of scrub typhus: A network meta-analysis and cost-effectiveness analysis.

Introduction: A pharmacoeconomic analysis model was developed to evaluate the cost-effectiveness of antibiotics from a societal perspective in Korea and China. A network meta-analysis was conducted to evaluate the efficacy of antibiotics.

Methodology: We conducted a systematic search for randomized controlled trials or quasi-randomized controlled trials on antibiotics employed as therapy in scrub typhus management.

Correlations of Gelling Properties and 3D Printability to the Chemical Composition and Rheological Properties of Surimi from Different Marine Fish Species.

Samples of marine surimi made from six different fish types have been comprehensively investigated and compared in terms of chemical composition, protease activity, gelling chemistry, gel properties and 3D printability. It has been found that surimi with high protein content, low fat concentration, low activity of endogenous protease (cathepsin B, cathepsin L, cathepsin D, calpain and serine protease) and low TVB-N showed better gelling capacity and gel properties. However, the 3D printability of surimi was more relevant to apparent viscosity.

Phosphorylation of POU3F3 Mediated Nuclear Translocation Promotes Proliferation in Non-Small Cell Lung Cancer through Accelerating ATP5PF Transcription and ATP Production.

Targeting oxidative phosphorylation (OXPHOS) through inhibiting the electron transport chain (ETC) has shown promising pre-clinical efficacy in cancer therapy. Although aerobic glycolysis is a hallmark of cancer, emerging evidence suggest OXPHOS is frequently enhanced, providing metabolic advantages for cell proliferation, metastasis, and drug resistance in a variety of aggressive cancer types including non-small cell lung cancer (NSCLC), yet the underlying molecular mechanisms remain elusive. Here it is reported that POU-domain containing family protein POU3F3 is translocated into the nuclei of NSCLC cell lines harboring mutant RAS, where it activates transcription of ATP5PF, an essential component of mitochondrial ATP synthase and consequent ATP production, leading to enhanced NSCLC proliferation and migration.

A risk factor prediction model for moderate-to-severe postoperative pain in patients undergoing laparoscopic sleeve gastrectomy.

The primary goal of this study was to identify the risk factors contributing to moderate-to-severe postoperative pain in patients undergoing laparoscopic sleeve gastrectomy (LSG) and to create a predictive model for these risk factors. A retrospective analysis was performed on a cohort of 375 patients who underwent LSG at Jinan Central Hospital from January 2017 to June 2023. Data for this study was extracted using medical databases.

Response of intercropped maize "source-sink" characteristics to elevated CO concentration and phospho-rus application in maize intercropping with peanut system.

This study aimed to clarify the impacts of elevating CO concentration (e[CO]) on maize "source-sink" characteristics in maize intercropping with peanut system. We investigated the impacts of e[CO] on leaf "source" quantity, gas exchange parameters, population "source" productivity, "sink" capacity, grain to leaf ratio, yield and intercropping advantage of maize under 0 (P) and 180 kg PO·hm(P). The results showed that compared with ambient CO concentration (a[CO]), e[CO] augmented leaf "source" quantity and "source" activity of intercropping maize, and significantly increased productivity of population "source".

Discovery of natural products as influenza neuraminidase inhibitors: in silico screening, in vitro validation, and molecular dynamic simulation studies.

Influenza is a highly contagious respiratory illness that imposes a significant global burden. Antiviral neuraminidase inhibitors (NAIs) such as oseltamivir (OC) have been proven essential, but the emergence of resistant viral strains necessitates the development of novel therapies. This study explored the potential of natural products as alternative NAIs.

The Characteristics and Functions of Orally Absorbed Herbal Decoction-Borne Plant MicroRNAs.

Herbal decoctions always contain numerous plant microRNAs, and some of these can be absorbed orally to exert cross-kingdom gene regulation. However, little is known about which specific types of herbal decoction-borne plant microRNAs are more likely to be absorbed. Thus, two antiviral herbal decoctions, Qingfei Paidu and Qingre Huashi Kangdu, were administered to human volunteers and rats, respectively, to investigate the characteristics of orally absorbed decoction-borne plant microRNAs.

Anti-Osteoporosis Is Imperative in Prevention of Progress of Ankylosing Spondylitis.

Background: Ankylosing spondylitis (AS) is a chronic autoimmune disease that affects the spine and peripheral joints, often leading to kyphosis, joint stiffness, and even ankylosis. Sagittal parameters, such as total thoracic kyphosis (TTK), thoracic kyphosis (TK), major thoracic kyphosis (MTK), and thoracolumbar kyphosis (TLK), are crucial indices for evaluating spinal alignment in AS patients and can reflect disease progression. This study aims to explore the relationship between bone mineral density (BMD), sagittal parameters, and joint ankylosis in AS patients.

Efficacy and safety of long fusion versus short fusion in degenerative scoliosis: a systematic review and meta-analysis.

Background: This study aimed to evaluate the efficacy and safety of long fusion versus short fusion in patients with degenerative scoliosis.

Methods: Databases were systematically searched up to June 2024. The authors applied Review Manager 5.

Comparison of the efficacy of LTCBDE and LCBDE for common bile duct stones: a systematic review and meta-analysis.

Background: The choice of surgical methods for common bile duct stones (CBDS) is controversial. The aim of this study was to compare the safety and efficacy of laparoscopic transcystic common bile duct exploration (LTCBDE) and laparoscopic common bile duct exploration (LCBDE).

Methods: Relevant literature published before March 30, 2023 in PubMed, Web of Science, Embase, and Cochrane was searched to screen studies comparing LTCBDE and LCBDE.

Cost-effectiveness analysis of durvalumab with chemotherapy and maintenance durvalumab with or without olaparib for advanced endometrial cancer.

This study evaluates the cost-effectiveness of adding durvalumab to chemotherapy, with subsequent maintenance either with olaparib (DOCT) or without olaparib (DCT), versus chemotherapy alone (CT) as a first-line treatment for advanced endometrial cancer (EC) in the United States, stratified by mismatch repair deficiency (dMMR) and proficiency (pMMR). A Markov model based on DUO-E Phase III trial data simulated disease progression and outcomes. Total costs, quality-adjusted life years (QALYs), and incremental cost-effectiveness ratios (ICER) were evaluated.

Discovery of a heparan sulfate binding domain in monkeypox virus H3 as an anti-poxviral drug target combining AI and MD simulations.

Viral adhesion to host cells is a critical step in infection for many viruses, including monkeypox virus (MPXV). In MPXV, the H3 protein mediates viral adhesion through its interaction with heparan sulfate (HS), yet the structural details of this interaction have remained elusive. Using AI-based structural prediction tools and molecular dynamics (MD) simulations, we identified a novel, positively charged α-helical domain in H3 that is essential for HS binding.

Enhancing the psychological well-being and sleep quality of healthcare providers with a multimodal psychological support program: a randomized controlled trial.

Background: The COVID-19 pandemic significantly challenged the global healthcare system, especially frontline healthcare professionals, such as those working in intensive care units (ICUs). In late 2022, a sudden increase in COVID-19 cases in China led to a large number of ICU admissions, requiring new ICU staff (non-ICU professionals to work in ICUs), exacerbating their stress. This study aimed to develop an effective stress management strategy for new ICU professionals, focusing on reducing the detrimental effects of stress on their psychological state.

Factors influencing surimi gelling properties and natural additive-based gel fortification strategies: A review.

Gelation and gel properties are crucial to surimi-based seafood products, and many factors significantly influence surimi gel quality. Although physical and chemical modifications can improve surimi gel performance, challenges such as high cost, difficulties in industrialization and environmental pollution pose significant barriers to their practicality. Natural additives offer a promising alternative by reinforcing and improving the characteristics of surimi gel through mechanisms such as protein conformational transformation, protein denaturation, and altered chemical forces.

Genetic Diversity and Fingerprinting of 231 Mango Germplasm Using Genome SSR Markers.

Mango ( L.) (2n = 40) is an important perennial fruit tree in tropical and subtropical regions. The lack of information on genetic diversity at the molecular level hinders efforts in mango genetic improvement and molecular marker-assisted breeding.

Do Larger Pollinators Have Higher Pollination Efficiency for the Generalized Pollination Plant ?

To understand the reproductive strategies of the typically introduced plant and to compare the pollination efficiency of its different pollinators, we observed, measured, and recorded the flowering dynamics, floral traits, and visiting insects of . Furthermore, we compared the body size, visitation rate, and pollination efficiency of the pollination insects of . The results indicated that, despite exhibiting specialized moth pollination characteristics based on similarities in flower features to other moth-pollinated species, actually presented a generalized pollination system.

Cost-effectiveness analysis of anlotinib plus chemotherapy with or without benmelstobart versus chemotherapy alone for extensive-stage small-cell lung cancer in China.

Objectives: The ETER701 trial demonstrated that benmelstobart combined with anlotinib and etoposide-carboplatin (EC) significantly extends survival in patients with extensive-stage small cell lung cancer (ES-SCLC), setting a new record for median overall survival. In contrast, anlotinib plus EC only significantly prolongs progression-free survival. However, there is currently no evidence evaluating the cost-effectiveness of these regimens as first-line treatments.

Emerging Trends on the Growing Rods in Scoliosis in the Last 20 Years: A Bibliometric Analysis from 2003 to 2024.

Background: Growing rods is a distraction-based, growth-friendly method that is commonly used in the treatment of scoliosis.

Methods: Studies on growing rods in scoliosis published from 2003 to 2024 were retrieved from the Web of Science Core Collection. VOSviewer, Microsoft Excel, and CiteSpace were applied for data analysis.