Comprehensive lipidomics and flavoromics reveals the accelerated oxidation mechanism of fish oil from silver carp (Hypophthalmichthys molitrix) viscera during heating.

This study employed gas chromatography-mass spectrometry (GC-MS) and lipidomic approaches to analyze volatile organic compounds (VOCs) and lipid dynamics in silver carp visceral fish oil during accelerated oxidation at 60 °C. The lipidomic profiling revealed 1362 distinct lipid molecules, encompassing 92 fatty acids. Triglycerides (TGs) underwent degradation in the early oxidation phase (0-6 days), whereas glycerophospholipid breakdown dominated the later stages (9-20 days).

Clinical and electrophysiological features of pure sensory Guillain-Barré syndrome: retrospective analysis of 22 patients across 14 provinces in Southern China.

Objective: Currently, there are limited reports, both nationally and internationally, regarding Guillain-Barré Syndrome (GBS) that manifests solely with isolated sensory impairment. This study aims to explore the epidemiological and clinical features of GBS patients experiencing only paresthesia in southern China.

Methods: We conducted a retrospective analysis of the medical records of GBS patients admitted to 31 hospitals across 14 provinces in southern China from January 1, 2013, to September 30, 2016.

Achieving efficient co-expression of endo- β-1,4-xylanase and α-arabinofuranosidase in Trichoderma reesei and application in the production of arabino-xylo-oligosaccharides.

A feasible process for the degradation of psyllium arabinoxylan (AX) to arabino-xylo-oligosaccharides (AXOS) is proposed. A suspension of Trichoderma reesei mycelium suspension, acting on seed husks of psyllium as a carbon source, produced endo-β-1,4-xylanase (xynTR) and α-arabinofuranosidase (abfTR), with enzymatic activity levels of 11.09 ± 0.

Integration of lipidomics and flavoromics reveals the lipid-flavor transformation mechanism of fish oil from silver carp visceral with different enzymatic hydrolysis.

Lipidomics and volatile organic compounds (VOCs) of fish oil from silver carp visceral hydrolyzed by papain, alkaline protease, pepsin, neutral protease, flavor protease, trypsin, and complex protease were investigated to explore the lipid-flavor transformation mechanism. Our results showed that diglycerol (DG, 18:4/16:1, 18:3/18:3, 16:0/18:3)/fatty acids (20:4) in glycerides and phosphatidylethanolamine (PE, 4:0/18:2)/phosphatidylglycerol (8:0e/10:3) in phospholipids could be used as markers to distinguish fish oil by different enzymolysis. A total of 130 and 36 VOCs were detected in fish oil using GC-MS and GC-IMS, respectively, including 11 key VOCs, namely 1-octen-3-ol, 1-heptanol, eugenol, (E)-2-decenal, (E, E)-2,4-heptadienal, (E, E)-2,4-decadienal, (E)-2-nonenal, benzene acetaldehyde, and 2,3-pentanedione.

Metabolic reprogramming shapes post-translational modification in macrophages.

Polarized macrophages undergo metabolic reprogramming, as well as extensive epigenetic and post-translational modifications (PTMs) switch. Metabolic remodeling and dynamic changes of PTMs lead to timely macrophage response to infection or antigenic stimulation, as well as its transition from a pro-inflammatory to a reparative phenotype. The transformation of metabolites in the microenvironment also determines the PTMs of macrophages.

Identification and complete genome sequence of honeysuckle-associated cytorhabdovirus in China.

A putatively novel cytorhabdovirus, tentatively named "honeysuckle-associated cytorhabdovirus" (HaCV), was identified by high-throughput sequencing in honeysuckle plants exhibiting chlorosis and mottling symptoms. Five isolates were obtained in this study, all of which were found to have a similar genome structure, ranging in length from 13,891 to 13,930 nucleotides and containing eight open reading frames (ORFs). In addition to ORFs encoding the five canonical proteins N, P, M, G, and L, these isolates had an ORF within the P gene, one between the P and M genes, and one between the G and L genes, encoding the putative proteins P', P3, and P6, respectively.

Impact of online health education on attention-deficit/hyperactivity disorder screening results and parenting stress among school-aged children.

Aims: To investigate the effects of an online health education lecture on the positive screening rate of attention-deficit/hyperactivity disorder (ADHD) and parenting stress among parents of children diagnosed with or screened positive for ADHD.

Methods: Using stratified proportional random cluster sampling, 14 primary schools in Chongqing were selected to conduct an online lecture about ADHD for parents and teachers. A total of 2,611 questionnaires were collected (1,508 intervention group, 1,103 control group).

Unraveling the trisubstrate-triproduct reaction mechanisms of wine grape VvCYP76F14 to improve wine bouquet.

Bouquet is a captivating wine characteristic that affects consumer consumption. Wine lactone correlates with both precursor ((E)-8-carboxylinalool) concentration and wine age that has the greatest contribution to bouquet. In wine grape, cytochrome P450 VvCYP76F14 can catalyze trisubstrate-triproduct reaction processes (hydroxylation, dehydrogenation and carboxylation) to produce (E)-8-carboxylinalool.

Epidermal Mechanical Scratching-Induced ROS Exacerbates the Itch-Scratch Cycle via TRPA1 Activation on Mast Cells in Atopic Dermatitis.

Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by the itch-scratch cycle. Itching, induced by irritants or allergens that stimulate pruriceptive neurons, triggers uncontrollable mechanical scratching, leading to epidermal barrier disruption, immune response activation, inflammatory mediator release, and further stimulation of pruritus conduction. Although oxidative stress and immune cells can exacerbate this cycle, the correlation between mechanical scratching, epidermal oxidative stress, and dermal mast cell activation in AD remains unclear.

Autotransplantation of a Third Molar to Replace Compromised Molar With the Individual Three-Dimensional Printed Ultrasonic Osteotome: A Case Report.

Tooth autotransplantation is widely used to replace congenitally missing teeth or teeth with irreversible damage. This case report presents a personalized ultrasonic osteotome that enables precise preparation, minimizes bone trauma, enhances the initial stability of the transplanted tooth, and contributes to a favorable prognosis. The procedure is as follows: a 25-year-old female patient presented with a porcelain-fused-to-metal crown on Tooth #19, which had detached due to severe decay, rendering the tooth unsalvageable.

Identification of SETD4 as an Onco-Immunological Biomarker Encompassing the Tumor Microenvironment, Prognoses, and Therapeutic Responses in Various Human Cancers.

Background: SET domain-containing protein 4 (SETD4) is a histone methyltransferase that has been shown to modulate cell proliferation, differentiation, and inflammatory responses by regulating histone H4 trimethylation (H4K20me3). Previous reports have demonstrated its function in the quiescence of cancer stem cells as well as drug resistance in several cancers. A limited number of systematic studies have examined SETD4's role in the tumor microenvironment, pathogenesis, prognosis, and therapeutic response.

Observation of Large Low-Field Magnetoresistance in Layered (NdNiO):NdO Films at High Temperatures.

Large low-field magnetoresistance (LFMR, < 1 T), related to the spin-disorder scattering or spin-polarized tunneling at boundaries of polycrystalline manganates, holds considerable promise for the development of low-power and ultrafast magnetic devices. However, achieving significant LFMR typically necessitates extremely low temperatures due to diminishing spin polarization as temperature rises. To address this challenge, one strategy involves incorporating Ruddlesden-Popper structures (ABO):AO, which are layered derivatives of perovskite structure capable of potentially inducing heightened magnetic fluctuations at higher temperatures.

Glyphosate-Based Herbicide Stress During Pregnancy Impairs Intestinal Development in Newborn Piglets by Modifying DNA Methylation.

Glyphosate-based herbicide (GBH), a feed contaminant, has been proven to impair the growth and development of humans and animals. Previous research has revealed that maternal toxin exposure during pregnancy could cause permanent fetal changes by epigenetic modulation. However, there was insufficient evidence of the involvement of DNA methylation in maternal GBH exposure-induced intestinal health of offspring.

Simple and sensitive monitoring of polycyclic aromatic hydrocarbons in edible oils by polydimethylsiloxane/pyrazine-based hyper-crosslinked polymer coated stir bar sorptive extraction followed by gas chromatography-mass spectrometry detection.

Background: Edible oils are susceptible to contamination with polycyclic aromatic hydrocarbons (PAHs) throughout production, storage, and transportation processes due to their lipophilic nature. The necessity of quantifying PAHs present in complex oil matrices at trace levels, which bind strongly to impurities in oil matrices, poses a major challenge to the accurate quantification of these contaminants. Therefore, the development of straightforward and effective methods for the separation and enrichment of PAHs in oil samples prior to instrumental analysis is paramount to guaranteeing food safety.

Meta-Analysis of the Clinical Efficacy and Safety of Unilateral Biportal Endoscopic Lumbar Interbody Fusion versus Endoscopic Lumbar Interbody Fusion for the Treatment of Lumbar Degenerative Diseases.

Objective: To compare the efficacy and safety of unilateral biportal endoscopic lumbar interbody fusion (UBE-LIF) and endoscopic lumbar interbody fusion (Endo-LIF) in the treatment of lumbar degenerative diseases.

Methods: A thorough literature search was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines and the PICO framework (PROSPERO 2024CRD42024592073). The databases searched included PubMed, Embase, Web of Science, Cochrane Library, China National Knowledge Infrastructure, and Wanfang Database, with a time frame of January 2020 to June 2024.

Experimental investigation into the Impact of Cyclic High Low Pressure Water Immersion on the Pore structure and Mechanical properties of coal.

The extraction of coal seams with high gas content and low permeability presents significant challenges, particularly due to the extended period required for gas extraction to meet safety standards and the inherently low extraction efficiency. Hydraulic fracturing technology, widely employed in the permeability enhancement of soft and low-permeability coal seams, serves as a key intervention. This study focuses on the high-rank raw coal from the No.

Enhanced Gaussian Process Model for Predicting Compressive Strength of Ultra-High-Performance Concrete (UHPC).

Ultra-high-performance concrete (UHPC) is widely used in engineering due to its exceptional mechanical properties, particularly compressive strength. Accurate prediction of the compressive strength is critical for optimizing mix proportions but remains challenging due to data dispersion, limited data availability, and complex material interactions. This study enhances the Gaussian Process (GP) model to address these challenges by incorporating Singular Value Decomposition (SVD) and Kalman Filtering and Smoothing (KF/KS).

The Intelligence Structures of School-Age Children with Attention Deficit Hyperactivity Disorder: A Multicenter Cross-Sectional Study in China.

Purpose: There is no consensus on whether cognitive measures among attention deficit hyperactivity disorder (ADHD) subtypes exhibit more similarities or differences, and most of them have been conducted on English-speaking subjects, lacking cross-cultural perspectives. The present study was aimed at investigating the intelligence structures of school-age children with ADHD who speak Chinese, using a multicenter and large sample size approach, offering some references for clinicians.

Patients And Methods: 772 children aged 6 to 12 years with ADHD took part in the cross-sectional study.

Characterization of global research trends and prospects on celastrol, a principal bioactive ingredient of Hook F: bibliometric analysis.

Context: Celastrol, acknowledged as a prominent exemplar of the potential for transforming traditional medicinal compounds into contemporary pharmaceuticals, has garnered considerable attention owing to its extensive pharmacological activities. The increasing volume of publications concerning celastrol highlights its importance in current scientific inquiry. Despite the growing interest in this compound, a bibliometric analysis focused on this subject remains to be undertaken.

Predicting the toxic side effects of drug interactions using chemical structures and protein sequences.

The study aims to address the critical issue of toxic side effects resulting from drug combinations, which can significantly increase health risks, clinical complications, and lead to drug being withdrawn from the market. A model named TSEDDI (toxic side effects of drug-drug interaction) has been developed to improve the identification of drug pairs that may induce toxicity or adverse reactions. By utilizing drug chemical structures and diverse proteins, we employ a convolutional neural network (CNN) to extract features from molecular images, enzyme proteins, transporter proteins, and target proteins.

Characterization of key off-odor compounds in grass carp cube formed during room temperature storage by molecular sensory science approach.

Flavor is a significant factor in determining the popularity of freshwater fish. However, freshwater fish can easily spoil during storage, producing an unpleasant odor. Little research has determined the changes in key off-odor compounds (OOCs) in freshwater fish during storage.

Arthroscopic V-Shaped Double-Pulley Suture-Bridge Repair of Massive Rotator Cuff Tear.

At present, suture bridge is a feasible choice in the treatment of massive rotator cuff tears (MRCTs). However, high tension on the repair site and medial tension during suture tightening and after medial knotting are unavoidable problems in MRCT repair with a suture bridge. Arthroscopic V-shaped double-pulley suture-bridge repair is a pragmatic surgical technique for the repair of MRCTs.

Gatifloxacin hydrochloride confers broad-spectrum antibacterial activity against phytopathogenic bacteria.

Bacterial diseases pose significant threats to agriculture and natural ecosystems, causing substantial crop losses and impacting food security. Until now, there has been a less efficient control strategy against some bacterial diseases such as bacterial wilt, caused by . In this study, we screened a library of 58 microorganism-derived natural products for their antibacterial activity against .