Neonatal hypothyroidism alters the kinetic properties of Na+, K+-ATPase in synaptic plasma membranes from rat brain.

Neonatal hypothyroidism was induced in rat pups by injecting 131I within two days of birth and the effects on kinetic properties of Na+, K+-ATPase from synaptic plasma membranes were examined. Neonatal hypothyroidism resulted in a generalized decrease in V(max) with ATP, Na+, K+ and Mg2+ together with an increase in the K(m) for ATP, appearance of a low affinity component for Na+ and allosteric characteristic for the Mg2+-dependent activity at high Mg2+ concentrations. Binding pattern for Na+ and Mg2+ changed.

Effect of neonatal hypothyroidism on the kinetic properties of Na+, K+ -ATPase from rat brain microsomes.

The effects of neonatal hypothyroidism on the kinetic properties of Na+, K+ -ATPase from rat brain microsomes were examined. Neonatal hypothyroidism resulted in decreased Na+, K+ -ATPase activity compared to control samples (7.4 +/- 1.

Insulin status differentially affects energy transduction in cardiac mitochondria from male and female rats.

Aim: The incidence of coronary heart diseases (CHD), congestive heart failure (CHF) and myocardial infarction is higher in diabetic patients than in non-diabetic groups, with these incidences being more in women than in the men. Hence, we examined involvement of mitochondrial energy transduction functions.

Methods: Mitochondrial energy metabolism in cardiomyopathy was studied using streptozotocin (STZ)-diabetic male and female rats as the model system.

Effect of neonatal hypothyroidism on Ca(2+)-ATPase activity in the rat brain.

Effect of neonatal hypothyroidism on kinetic properties of Ca(2+)-ATPase from rat brain synaptic plasma membranes and microsomes were examined. Neonatal hypothyroidism resulted in significant decrease in the enzyme activity in both the membrane systems. The synaptic membranes in control group displayed presence of one kinetic component whereas a low affinity component became evident in the hypothyroid group.

Non enzymatic glycosylation of alpha-1-proteinase inhibitor of human plasma.

Human plasma contains inhibitors, which control the activity of proteolytic enzymes. Alpha-1-proteinase inhibitor and alpha-2-macroglobulin are two of them present in high concentration in human plasma, which inhibit action of trypsin among other proteinases. The trypsin inhibitory capacity (TIC) of human plasma is observed to be decreased in pathological conditions like diabetes mellitus.

Hypolipidemic effects of synthetic gugulsterones in normal rats and assessment of its long-term toxicity at cellular levels in various organs.

Synthetic gugulsterones when administered to rats for a period of 3 weeks in dose of 5.0 mg/kg body weight/day caused a reduction in levels of total cholesterol by 30%, LDL-chol. by 40%, Tg by 40%.

Contraceptive potential of an antiprogestin ZK 98.734: reversal of ZK 98.734--induced blockade of folliculogenesis with FSH and LH and their differential effects in bonnet monkeys.

Studies were undertaken in adult bonnet monkeys to investigate whether treatment with an antiprogestin ZK 98.734 at weekly intervals, starting from day one of menstrual cycle, could arrest ovulation and also to determine if ZK 98.734 induced blockade of ovulation could be reversed with gonadotropins.

Possible involvement of the accessory sex glands in the regulation of pituitary hormones.

A time-course alteration in the blood levels of gonadotrophin was evaluated in male rats aged 49 days after: (a) removal of the seminal vesicles, (b) bilateral orchidectomy, or (c) bilateral orchidectomy together with removal of the seminal vesicles. Age-matched sham-operated animals served as controls. Removal of the seminal vesicles resulted in elevation in the blood levels of LH, FSH and prolactin compared to controls.

Spectrum of pediatric urolithiasis in western India.

Of 1,211 patients with urolithiasis treated at this institution over a nine years period, there were 77 (6.4%) pediatric cases. The commonest age group was 6-10 years (55.

Predictive value of some biochemical indices in stone formers.

Search is under way to develop reliable tests for the prediction of stone risk. Several indices and ratios on the basis of urinary excretions have been suggested. In the present study the applicability of some risk indices and ratios in slum dwellers of Dharavi area of Bombay was examined.

Urolithiasis in northeast Bombay: seasonal prevalence and chemical composition of stones.

The seasonal prevalence of urinary calculus disease from the records of L.T. Municipal General Hospital, Sion, Bombay, which primarily caters services to poor people of Northeast Bombay, especially Dharavi slum area, is reported.

Acute pancreatitis and reduction of H+ ion concentration in gastric secretions in experimental acute myocarditis produced by Indian red scorpion, Buthus tamulus, venom.

Crude venom (4 mg/kg) of scorpion (B. tamulus) was given in saline to anaesthetized dogs and rabbits. It produced a reduction in gastric H+ ion concentration in dogs with acute myocarditis.

Hepatic drug metabolizing capacity of patients of cirrhosis before and after clinico-biochemical recovery.

The present study was undertaken to determine whether, along with clinico-biochemical recovery there was associated restoration of hepatic drug metabolizing capacity in patients of cirrhosis after treatment of their cirrhosis, using serum antipyrine half life, the ideal index. Estimation of serum antipyrine half life before (26.34 +/- 2.

Serum-alpha-hydroxybutyrate dehydrogenase levels in sickle-cell disease and sickle-cell crisis.

In patients with sickle-cell disease (Hb S/S disease) who are not in crisis, the serum-level of alpha-hydroxybutyrate dehydrogenase (alpha-H.B.D.

Effects of a human plasma fraction on the release of chemotactic factors and anaphylatoxin from complement.

A fraction prepared from normal human plasma inhibits the release of chemotactic factors and anaphylatoxin when complement is activated by the alternate but not by the classical pathway. The fraction gave no interference with the actions of the released substances on either leucocytes or guinea-pig isolated ileum. The possible relation of this effect to the mechanism of the anti-inflammatory actions of the plasma fraction is discussed.