HEMATOPOIETIC PROGENITORS CELLS IN PERIPHERAL BLOOD OF BALB/C MICE UNDER IONIZING RADIATION ACTION IN SUBLETHAL DOSE.

Objective: determination of the content of hematopoietic progenitor cells circulating in peripheral blood of Balb/Cmice, under ionizing radiation action in sublethal dose, at different periods after the irradiation, using cell culturein diffusion chambers in vivo.

Methods: Peripheral blood smears of Balb/C mice were prepared and studied, its cellular composition was determined, as well as by cultivation of peripheral blood cells in diffusion chambers in vivo their colony-forming efficien-cy was determined on the 0th, 5th, and 30th day after external irradiation in sublethal dose 5.85 Gy.

NEW DATA ON HISTOGENESIS AND HISTOLOGICAL STRUCTURE OF LUNG CANCER.

Background: Lung cancer (LC) is one of the most common malignant neoplasms in men around the world, which poses a number of important challenges for scientists.

Aim: To analyze the histogenesis, features of the histological structure, and growth of LC.

Materials And Methods: The surgical material of 81 patients with LC was studied.

ANALYSIS OF THE INFLUENCE OF PROLONGED IRRADIATION ON HEMATOPOIETIC PROGENITOR CELLS IN GEL DIFFUSION CHAMBERS USING MATHEMATICAL MODELLING.

Objective: determining of the functional activity of mice bone marrow hematopoietic progenitor cells, cultivated in gel diffusion chambers, on the stages of hematopoiesis recovery after their prolonged irradiation in the lethal dose in a comparative aspect with the method of colony forming in spleen using mathematical model.

Materials And Methods: The method of cell cultivation in gel diffusion chambers, cytological methods, mathematical modeling, and statistical methods of research were used. Bone marrow samples extracted from the femur of mice irradiated with a total dose of 8 Gy with a power 0.

HUMANIZED MODEL OF ISOLATED SUSPENSION CULTIVATION OF HEMATOPOIETIC PROGENITOR CELLS FOR THE INVESTIGATION OF IONIZING RADIATION INFLUENCE IN VIVO.

Objective: development of the humanized system for cells cultivation outside the human organism (human-mouse)and investigation of the influence of ionizing radiation in increasing doses on the colony-forming ability ofhematopoietic progenitor cells.

Materials And Methods: Bone marrow samples of individuals without blood system diseases were cultivated in geldiffusion chambers with semi-solid agar in the abdominal cavity of CBA mice exposed to ionizing radiation action.Cell aggregates, which were obtained in the culture of diffusion chambers in vivo, were counted and colony-formingefficiency of bone marrow cells was determined.

COMPARATIVE MATHEMATICAL ANALYSIS OF COLONY-FORMING ABILITYOF THE BONE MARROW OF MICE LETHALLY AND NON-LETHALLY IRRADIATED WITH EQUAL DOSE RATE INTENSITY.

Objective: To perform comparative analysis of the characteristics of population functioning process of mice bonemarrow colony-forming units after their prolonged irradiation in lethal and non-lethal doses with equal dose rateintensity with the aid of mathematical model.

Materials And Methods: Assigned task is solved by means of mathematical model of alterations in the number ofbone marrow colony-forming units after continuous irradiation, described in previous works, with the use of experimental results of K. S.

ASSESSMENT OF RADIOPROTECTIVE ACTION OF BASIDIOMYCOTIC MELANIN PIGMENTS ON THE HEMATOPOIETIC SYSTEM OF Balb/C MICE UNDER EXPOSURE TO IONIZING RADIATION IN SUBLETHAL DOSE.

Objective: Assessment of radioprotective action of basidiomycotic melanin pigments on hematopoietic stem and progenitor bone marrow cells of Balb/C mice in case of exposure to ionizing radiation in sublethal dose.

Materials And Methods: Using original method of cultivation in gel diffusion chambers in vivo of bone marrow cells of Balb/C mice we investigated the colony-forming efficiency of hematopoietic progenitor cells of the ani- mals, which were exposed to ionizing radiation action in sublethal dose, in case of treatment with melanin pig- ments solution of basidiomycotic fungi as radioprotector.

Results And Conclusions: Investigation of functional activity of bone marrow progenitor cells of Balb/C mice allowed assessing their hematopoiesis state in case of ionizing radiation action, as well as in case of previous treat- ment of the animals with the solution of melanin pigments as radioprotector.

MATHEMATICAL ANALYSIS OF FUNCTIONAL PROPERTIES OF MICE BONE MARROW IN THE INITIAL PHASE OF ACUTE FRACTIONATED IRRADIATION.

Objective: to determine the quantitative characteristics of population functioning of mice bone marrow colony-forming units during seven days of acute fractionated irradiation.

Materials And Methods: Assigned task is solved by means of described in works R. V.

Mesenchymal stem and progenitor cells of rats' bone marrow under chronic action of ionizing radiation.

Unlabelled: Under the influence of ionizing radiation on hematopoietic system, the level of its injury is determined not only by the radiosensitivity of hematopoietic stem cells, but also by radiation induced changes in microenvironment func tioning, in particular, mesenchymal stem cells as its components.

Objective: to define functioning characteristics of mesenchymal stem and progenitor cells of rats' bone marrow under prolonged action of ionizing radiation as a result of 90Sr incorporation.

Materials And Methods: We applied the model of Wistar rats' internal irradiation with 90Sr radionuclide and per formed the in vitro cultivation of their bone marrow mesenchymal cells.

Circulating hematopoietic progenitor cells in patients affected by Chornobyl accident.

Unlabelled: High radiation sensitivity of stem cells and their ability to accumulate sublethal radiation damage provides the basis for investigation of hematopoietic progenitors using in vivo culture methodology. Unique samples of peripheral blood and bone marrow were derived from the patients affected by Chornobyl accident during liquidation campaign.

Aim: To investigate functional activity of circulating hematopoietic progenitor cells from peripheral blood and bone marrow of cleanup workers in early and remote periods after the accident at Chornobyl nuclear power plant (CNPP).

Pattern changes in quantitative and qualitative markers of hematopoietic stem cells during acute and chronic exposure to 90Sr isotope in cell culture.

Objective: The objective of our research was investigation of the hematopoietic system of laboratory rats under the influence of acute and chronic internal exposure to 90Sr isotope.

Materials And Methods: To study the condition of stem cells and their immediate progenitors we implemented cell culture methodology in vivo in gel diffusion capsules with subsequent analysis of the colonies and clusters.

Results: On the basis of experiments it was established that long-term effects of incorporated 90Sr isotope leads to significant disturbances in the hematopoietic system and in particular, revealing changes in hematological parameters of irradiated animals such as the appearance of circulating progenitor cells in peripheral blood, reducing the colony-forming efficiency of the bone marrow derived progenitor cells, as well as quantitative and qualitative changes in the clones.

[PREIMPLANTATION DEVELOPMENT OF HUMAN EMBRYOS WITH NUMERICAL CHROMOSOME ABNORMALITIES IN VITRO].

The study was focused on morphokynetic characteristics of in vitro cultured human embryos that were considered to be aneuploid or euploid according to the preimplantation genetic screening results. Among all the embryos examined only 34.2% were chromosomally balanced, while others possessed isolated or combined chromosome abnormalities.

Character of interaction between irradiated and non-irradiated cells in culture in diffusion chambers in vivo.

Objective: Current investigation is devoted to determination of interaction type between the non-irradiated murine bone-marrow cells and irradiated microenvironment in cell culture in diffusion chambers in vivo.

Materials And Methods: To study the characteristics of interaction between the cells we implemented an original cultivation model with bone-marrow cells of intact Balb/c mice inserted in the diffusion chambers in peritoneum of irradiated animals (it provided the access of nutrients and signaling molecules from outside).

Results: As a result of investigations performed, we have determined the direct influence of microenvironment factors of irradiated mice organism on the non-irradiated bone-marrow cells.

[Meiotic segregation of chromosomes 13 and 14 of heterozygous Robertsonian translocation der(13;14) (q10; q10) carriers].

Meiotic segregation of chromosomes 13 and 14 was assessed by fluorescence in situ hybridization on sperm of five heterozygous carriers of the most frequent Robertsonian translocation der(13;14). Alternate segregation mode was predominant (mean 78.2 +/- 5.

Determination of the optimal chemotherapy drugs pretreatment time through cultivation of hemopoietic cells in CML-patients treated with tyrosine kinase inhibitors.

Background: Targeted therapy drugs, including imatinib, are used for inhibiting the marker oncoprotein of chronic myeloid leukemia - BCR-ABL tyrosine kinase. However, in some patients the drug resistance can emerge too rapidly and a previous treatment with chemotherapy drugs can lead to formation of resistance.

Aim: To evaluate the influence of drugs that were used prior to the imatinib on the performance of the functional activity of bone marrow cells from chronic myeloid leukemia patients and their individual responses to therapy.

[Cardiomyocyte differentiation of individual clones murine induced pluripotent stem cells].

Cardiomyocyte differentiation of certain clones of murine induced pluripotent stem cells (iPS) was estimated. iPS were obtained due to reprogramming of murine embryonic fibroblasts with transposon-based Sleeping beauty plasmids as sene delivery systems. Differentiation was performed in suspension culture and in attached to tissue-culture plates embryoid bodies (EBs).

Chromosomal segregation in sperm of Robertsonian translocation carriers.

Purpose: To study meiotic segregation patterns of Robertsonian translocations in sperm of male carriers and to assess the frequencies of unbalanced sperm formation.

Methods: FISH with combination of probes to detect all the variants of meiotic segregation was performed on decondensed sperm nuclei of 5 carriers of der(13;14), 3 carriers of der(14;21) and one carrier of a rare der(13;21) translocation.

Results: The frequency of sperm with alternate segregation and normal/balanced chromosomal complement ranged from 68 % to 94.

Characterization of the interactions between stromal and haematopoietic progenitor cells in expansion cell culture models.

Development of the long-term culture models of haematopoietic stem cells (HSCs) is one of the important tasks in modern biotechnology. It has been suggested that stromal presence is important for haematopoiesis in vitro and in vivo, but the question remains: whether diffusible factors produced by stromal cells are sufficient for the regeneration of primitive and definitive haematopoietic cells, or direct cell-to-cell contacts of the cultured material with underlying stromal base would be required. During present studies, influence of various feeder layers and feeder layer conditioned media on proliferative, differentiative and clonogenic activity of human AC133+ derived from human umbilical cord blood was investigated.

[Erythroid precursor cells in a tissue culture from subjects exposed to ionizing radiation].

Cloning efficiency of erythroid progenitor cells in gel diffusion chambers from patients irradiated as a result of the Chernobyl accident was studied. Stimulation of erythroid progenitor cells, activation of enzymatic activity in peripheral blood cells and an increase of 2,3-D Ph G were detected. These data supplement the notion about the development of adaptative processes in the erythron system as a response to effects of radiation.

[Erythron system function in adults subjected to radiation exposure as a result of the Chernobyl accident].

The morphofunctional state of hemopoiesis in patients who suffered from the Chernobyl accident was investigated. The activation of proliferative activity of erythroid progenitor cells simultaneously with a decrease in the resistance of erythrocytes and an increase in the levels of methemoglobin, HbF, and an increase in the number of HbF-containing cells in peripheral blood were detected. Main trends in the development of adaptative processes in the erythron system as a response to affect of low doses of radiation were determined on cellular and molecular levels.

[Erythroid differentiation and specific protein synthesis by the leukemic cell line K-562 (under in-vivo cultivation conditions)].

Prolonged cultivation of K-562 human leukemic cells in plastagar diffusion chambers inserted into the peritoneal cavities of CBA mice indicated erythroid commitment and differentiation of cells. It was shown by the method of dot-hybridization with the use of oligonucleotide probe the presence of mRNA of globin in these cells.

[Cryopreservation of a suspension of fetal liver cells for clinical use].

The authors evaluate a method of procuring and preserving fetal liver cells in a medium containing low-molecular polyvynilpyrrolidon, glucose, lactose, sodium phosphate and sodiumhydrocitrate not requiring washing off. The biomaterial is stored at a temperature of -196 degrees C. The efficiency of this method of cryopreservation of fetal liver cell suspensions was confirmed by high morphological intactness, functional activity and proliferative capacity.